LABEL-FREE BINDING ANALYSIS MICROCALORIMETRY LABEL-FREE BINDING ANALYSIS MICROCALORIMETRY MICROCAL ITC SYSTEMS UNDERSTANDING BIOMOLECULAR INTERACTIONS

Understanding biomolecular interactions MEASURE MULTIPLE BINDING PARAMETERS IN A SINGLE EXPERIMENT Isothermal titration microcalorimetry (ITC) is an essential tool in drug discovery and the study and regulation of protein interactions. Having been developed specifically to meet the needs of life scientists working in these fields, Malvern MicroCal ITC calorimeters deliver the exceptional performance and outstanding quality data needed in these application areas. MicroCal ITC systems directly measure the heat released or absorbed during a biomolecular binding event. The result is a direct, label-free...

Understanding biomolecular interactions Applications Used widely in the life sciences and drug discovery with key applications in: Characterizing biomolecular interactions, to: • Confirm binding and activity • Determine stoichiometry and thermodynamic parameters • Study structure activity relationships Studying the interaction of any two biomolecules including: • Proteins, nucleic acids, lipids, drugs and inhibitors Drug discovery for: • Hit validation and characterization • Lead optimization • Mechanism of action Sample volume Sample cell size MicroCal PEAQ-ITC Automated Fully automated MicroCal...

Understanding biomolecular interactions INTRODUCTION TO ISOTHERMAL TITRATION MICROCALORIMETRY Isothermal titration microcalorimetry (ITC) measures the binding affinity and thermodynamics of biomolecular interactions, helping to understand why interactions occur. The technique is based on the measurement of heat evolved or absorbed when complexes are formed between molecules. It has the advantage of measuring all binding parameters in a single label-free, in-solution experiment, including binding affinity (K D), reaction stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). This reveals thermodynamic...

Understanding biomolecular interactions THE POWER OF ITC Isothermal titration calorimetry determines thermodynamic properties that tell you why interactions occur. Thermodynamic data reveal the forces that drive complex formation to describe function and mechanism at a molecular level. A. ITC determines thermodynamic properties including: the stoichiometry of Unfavorable the interaction (n), the affinity constant (K D), change in enthalpy (ΔH), and change in entropy (ΔS). G B. Shown are thermodynamic signatures of three interactions that have the same binding energy (ΔG). The binding energy is...

Understanding biomolecular interactions ITC IN ACTION – PROVEN VERSATILITY Theory into practice Thousands of citations in reference databases illustrate the diverse applications of MicroCal ITC systems. They are used to measure the binding affinity and thermodynamic properties of any biomolecular change that can influence recognition between binding partners. When combined with structural information, ITC data provide deeper insights into structure-function relationships and the mechanisms of binding. While the following examples provide a snapshot, you can find a wealth of detailed applications...

Understanding biomolecular interactions Optimizing enzyme kinetics with ITC The kinetics of xylanase is important in bio-bleaching wood pulp and biofuel production. Baumann and coworkers developed a system to measure xylanase kinetics with ITC since the traditional method was complicated and prone to systematic errors. The ITC method was also found to offer greater sensitivity and used less material. Typically, heat changes associated with xylan hydrolysis are too small to measure directly with ITC. To boost heat flow, an enthalpy amplification system that involved carbohydrate oxidase and catalase...

Understanding biomolecular interactions MICROCAL ITC RANGE AT A GLANCE MicroCal PEAQ-ITC Automated Combining exceptional performance of the MicroCal PEAQ-ITC with full automation and unattended operation, the MicroCal PEAQ-ITC Automated is a valuable asset for any busy research laboratory. User-friendly software ensures efficient experimental design while automated data analysis delivers fast, reliable results. The automation and throughput it offers make it a particularly good choice for drug discovery applications such as hit validation where productivity is crucial. FEATURES: • Fully automated...

Understanding biomolecular interactions MICROCAL ITC RANGE AT A GLANCE MicroCal PEAQ-ITC MicroCal PEAQ-ITC is designed for ease-ofuse and exceptional sensitivity. The wide affinity range enables analysis of weak to high affinity binders, with excellent reproducibility. MicroCal PEAQ-ITC analysis software offers experiment design simulation, batch evaluation of large data sets, automated assessment of data quality and a streamlined user interface that guides the user to final figures and presentation quality graphs quickly and easily. MicroCal ITC is an essential tool for any research laboratory...

Understanding biomolecular interactions MICROCAL ITC RANGE AT A GLANCE MicroCal iTC200 The MicroCal iTC200 is designed for ease of use and exceptional sensitivity. Its wide affinity range enables analysis of weak to high affinity binders with excellent reproducibility. Syringe and cell cleaning functions are semi-automated and require minimal operator involvement. User-friendly software guides all operations for fast accurate analysis. MicroCal iTC200 is an essential tool for any research laboratory studying biomolecular interactions where high sensitivity and rapid results are paramount. FEATURES:...

Understanding biomolecular interactions FAST AND ACCURATE ANALYSIS INSTRUMENT CONTROL SOFTWARE THAT TAKES YOU FROM EXPERIMENT TO FINAL RESULTS: MicroCal PEAQ-ITC instrument control software incorporates all the tools you need to go from experimental design to final results quickly and easily. ©wwiew AdjuM SKCI'HC Assign Carom It Adjust fit H nnuhfdblr ^^^^^fl bcMfcrWcfl Injrtlkjo taWe HjCntm v*s\ Siqiwlurr Plc( Haw ffcrt Intoned Ms* Pkfl Mddr Ratio Molar Ratio Molar Ratio GUIDED WORK-FLOWS User-friendly guided workflows with embedded help videos give any level of user the ability to generate...