iSWAB-Blood
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Blood Drop Collection Simplified Patented sample collection device iSWAB-Blood is an efficient system for the concentration and long term room temperature stabilization of gDNA and analytes from blood drops. Investigation of metabolic genetic disorders has traditionally been performed by collecting several blood drops through a minimally invasive procedure called a heel prick. This technique involves spotting 5-6 blood drops on filter paper. Despite its widespread use, there are several drawbacks to these sample collection methods: Long drying time after collection (2-3 hrs) Proper collection requires the blood drop to go through both sides of the paper within a designated circle (30-40% resampling rate) Low yields and highly fragmented DNA (< 5 kb) unsuitable for NGS, epigenetics, or microarray analysis Laborious and time consuming sample processing including card punching and placement into a tube or 96-well plate iSWAB-Blood overcomes the limitations of filter-paper based blood drop collection. The device is equipped with a squeezing insert to enable the efficient removal of blood from the swab, and into a proprietary DNA, RNA, and metabolite stabilizing solution for long term room temperature storage and transport. Real Time Stability Testing at Room Temperature Features & Benefits Collect - Concentrate - Transport - Extract - Analyze - Store: All within a single tube: Reduce processing time, storage real estate Liquid blood collection: No drying time required, <5 min collection process Collected sample is ready for room temperature shipment and long term storage with no special conditions (i.e. humidity control or air tight envelopes) Unlike filter cards, extracting DNA or measuring blood analytes does not require any front end prep and/or card punching DNA extraction can be performed with a standard whole blood purification protocol, yielding 2- 8 µg of high MW gDNA) Easy access to the sample for screening and/or in depth analysis purposes (genomics & metabolomics) – reduces resampling and assay failures Manual and automation compatible with no pre-prep process LIMS-compatible unique barcodes included on each iSWAB device for efficient traceability, chain of custody, and storage purposes DNA extraction was performed with QiaAMP Blood Extraction Kit Pooled ISWAB-Blood samples (n=24) was subjected to actual non-refrigerated airfreight sample shipping conditions, then stored at room temperature. An aliquate of 200 µL from the pooled sample was processed at each time point with QiaAMP Blood mini (whole blood protocol), then 2 µL of 100 µL elute was used for electrophoresis. High Molecular Weight gDNA from iSWAB-Blood collected samples Agarose gel (0.8%) electrophoresis of gDNA samples isolated from 100 µL of Human blood collected with ISWAB-Blood Spot using QiaAMP Blood kit. 4 µL of 100 µL elute was used for electrophoresis. M: λDNA/Hind III+EcoR I iSWAB-Blood collected samples provide long fragment genomic double stranded DNA mawi Biosampling Reinvented™ For research use only. Not for use in diagnostic procedures. iSWAB is a trademark of Mawi DNA Technologies LLC. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. © 2015 Mawi DNA Technologies LLC. All rights

1 Yields determined by Qubit: Average yield of volume equivalent of 250 pL volume of blood or dried blood spots were processed with a QIAamp Blood Mini Kit. 2 Measured by Nanodrop 3 Sample processing includes dry time and prepping before extraction / 96 samples iSWAB-Blood outperforms filter paper-based blood spot collection in all aspects References: Stangegaard, M., B0rsting,C., Ferrero-Miliani, L., Frank-Hansen, R., Poulsen, L., Anders J. Hansen, A. J., and Morling., N. Evaluation of Four Automated Protocols for Extraction of DNA from FTA Cards. Journal of Laboratory Automation 18(5)...