ISWAB-ID-250

ISWAB-ID-250

ISWAB-ID-250

Product catalog summary
Introduction: The document discusses the challenges associated with traditional methods of collecting biometric or crime scene samples, such as swabs and FTA cards, which can lead to errors or unusable samples. It highlights the need for improved collection methods that ensure sample integrity through stabilization at the point of collection, room temperature shipment, and long-term storage.
Challenges with Traditional Methods: Key issues include improper storage and transport leading to bacterial or mold growth, degradation of DNA by DNases and RNases, and over-drying causing irreversible DNA binding. These factors can compromise sample integrity and result in insufficient evidence for multiple assays.
iSWAB-ID Device: The iSWAB-ID device is presented as a next-generation forensic sample collection tool that addresses these challenges. It offers several advantages:
  • Collection: Suitable for Touch DNA and reference samples like buccal cells, blood spots, sweat, semen, and other body fluids.
  • Concentration and Stabilization: Ensures complete release and stabilization of samples from the swab into the iSWAB tube without drying time.
  • Record: Pre-barcoded components maintain the chain of custody.
  • Transport and Store: Allows room temperature transport and long-term storage.
  • Analyze: High DNA recovery supports multiple runs of Direct PCR, Genotyping, and DNA sequencing (Sanger & NGS).
Performance and Compatibility: iSWAB-collected samples remain stable for over five years at room temperature. The device is compatible with various Direct PCR-STR kits, including AmpFLSTR® Identifiler® and PowerPlex® systems. DNA extraction from blood collected with iSWAB shows high molecular weight gDNA and significant yields confirmed by Nanodrop and picogreen assays.
Conclusion: The iSWAB-ID device represents a significant advancement in forensic sample collection, offering improved sample integrity and compatibility with existing analysis methods. It is intended for research use only and not for diagnostic procedures.
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Catalog excerpts

ISWAB-ID-250-1

Next Generation Forensics Sample Collection Device Collect - Concentrate - Stablize - Record - Transport - Store - Analyze: All with one tube The collection of biometric or crime scene samples is a key step in the investigation process. As critical as this step is, however, traditional collection tools such as swabs and FTA cards leave significant room for error or unusable samples. After collection, inadequate resources can often lead to a backlog of several weeks or months to process the samples therefore increasing the possibility of yielding invalid results. Factors which can negatively impact sample integrity include - Improper storage and transport can lead to bacterial or mold growth - DNases and RNases released by collected cells can degrade DNA rapidly - Over drying of the sample can result in the irreversible binding of DNA In addition, there is often not enough evidence to support multiple assays. Therefore it is clear that a collection method that offers stabilization at the point of collection and allows room temperature shipment and long-term storage is critical for maintaining the integrity of the samples. iSWAB patented sample and concentration & stabilization technology The iSWAB-ID device represents a significant advancement in sample collection and stabilization technology. Our proprietary system overcomes the hurdles associated with current methods and offers the following advantages: Collect: Can be used for the collection of Touch DNA & Reference Samples (e.g. buccal cells, blood spots, sweat, semen, and any other body fluid) Concentrate and Stabilize: Complete release and stabilization of the sample from the swab into the iSWAB tube at the point of collection (no drying time required) Record: Pre-barcoded components to maintain chain of custody Transport and Store: Room temperature transport and long term storage Analyze: High DNA recovery allows for several runs of Direct PCR, Genotyping and DNA sequencing (Sanger & NGS) iSWAB Collected Samples are Stable Over 5 Years at Room Temperature Storage For research use only. Not for use in diagnostic procedures. iSWAB is a trademark of Mawi DNA Technologies LLC. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. © 2013 Mawi DNA Technologies LLC. All rights reserved. Version ISFDS-1.01

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ISWAB-ID-250-2

Next Generation Forensics Sample Collection Device iSWAB is compatible with Blood Spot Collection and Stabilization DNA Quality from Blood Collected with iSWAB Average Yield from Blood Collected with iSWAB 2.5 A260/280 A320/260 DNA extraction was performed with QiaAMP Blood extraction kit (n=12): The yields are from a single iSWAB tube. Blood drops were collected using 2 cotton swabs. Blood from the swabs was released and suspended in the iSWAB tube. No measurable DNA concentration was obtainable from equivalent volume of blood collected with FTA cards. DNA yields were confirmed with 2 different...

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All Mawi DNA Technologies catalogs and technical brochures

  1. iSWAB-Blood

    2  Pages

  2. iSWAB - Cells

    3  Pages

  3. iSWAB - DNA

    2  Pages

  4. ISWAB-DSC

    2  Pages

  5. ISWAB-RNA-v2

    1  Page

*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.