iSWAB - RNA v2 ™
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iSWAB - RNA v2 ™ - 1

The iSWAB-RNAv2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. The need to collect blood results in a very limited shelf life, forcing labs to invest in cold storage infrastructure or commit resources to immediate sample processing. Other options for non-invasive collection also suffer from short shelf life storage as well as low quality and unusable RNA. Applications include: • Real-time quantitative PCR • reverse transcription PCR • microarrays • Northern blot analysis • Nuclease protection assays • cDNA library construction • RNA-seq Buccal RNASeq Profile of Person 1 (PI) and Person 2 (P2) Features & Benefits • Collect - Stabilize - Concentrate - Transport - Store: At ambient temperature • Swab-free sample transport: Decrease sample processing time without compromising sample integrity • Increase process efficiency: Extended shelf life stability (up to 4 weeks at room temp, several years at -20 C and -80 C), reduced processing time, and minimal storage footprint • High quality RNA for downstream applications such as RT-PCR Microarray and RNA-seq for gene expression analysis • Room temperature stable: Reduce sample storage and transport costs by eliminating cold chain requirements • Self collection or assisted collection in less than 2 minutes: Suitable for all population segments including infants, toddlers, and elderly • Traceable and reliable chain of custody: LIMS compatible unique barcodes included on each collection device for efficient traceabil ity and storage purposes RNA Extraction: Zymo Quick-RNA™ MiniPrep Plus (Cat. Nos. R1057 / R1058) Data Analysis: The figure is a correlation matrix of the FPKM read counts for every gene expressed in the RNASeq data, clustered by similarity (Pearson correlation). RNASeq conditions: 60 million single end 50 base pair reads on an Illumina HiSeq using the Illumina TruSeq RiboZero stranded RNASeq kit Summary: • The quality of RNA obtained from iSWAB-RNA v2 is compatible with RNAseq based applications • Expression profiles of different subjects can be detected with RNAseq from buccal cells collected non-invasively • Scalable and easy to process: Manual and automation friendly sample processing • iSWAB-RNAv2 can efficiently stabilize buccal cells at room temperature for at least 10 days - No Cold Chain involvement For research use only. Not for use in diagnostic procedures. iSWAB is a trademark of Mawi DNA Technologies LLC. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. © 2013 Mawi DNA Technologies LLC. All rights reserved. Version ISR2DS-1.00

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