High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface Application Note Effects Blender 100 Series atmospheres on MCQ Gas of modified Application lab-grownmixtures applied on fungi cultures Dynamic Deposition techniques Introduction Fungi is a term that refers to a large family of organisms (mushroom, yeasts, moulds etc..), which counts more then 1.5 million species on Earth. Fungi have a strong link with most terrestrial (and some aquatic) ecosystems. Fungi act as a major decomposer, degrading organic matter to inorganic molecules which are re-integrated in the biochemical cycles through assimilation by plants or other organisms. The human use of fungi is practice rooted in the past which is currently extremely extensive. Food-related applications are one of the major example, for mushroom farming and gathering represent a large industries in many countries. New, interesting application fields have been discovered through the years, all of them connected with the capacity of fungi to produce a wide range of products with important biological properties. Nowadays a large share industrial production of antibiotics, vitamins and anti-cancer cholesterol-lowering drugs relies on cultured fungi. Productivity and quality of fungi cultures strongly depend on growing conditions. micrograph of Penicillium and its process A CVD chamber during the deposition spores. Among all the parameters that affect a culture, the surrounding atmosphere composition is one of the most crucial. Fungi are cultured in aerobic or anaerobic conditions, and most recently the use of modified atmosphere (greatly different from the standard air composition) have been implemented. For all these reasons, R&D demands professional instruments in order to perform accurate studies on fungi culture, and the MCQ Instruments faces these needs with its Gas Blender 100 Series. Highly accurate, reliable and easy to use, the MCQ Gas Blender 100 is the ideal instrument to create 3 component gas mixtures and to manage them dynamically. Pharmaceutical applications Natural compounds expression Fungi produce a wide array of natural compounds with useful biological activities that are of great interest for pharmaceutical applications. Fungi expression capacity is used for industrial production of metabolites that are the major source of pharmacologically active drugs. The major example of fungi culture application is the production of antibiotics, especially the penicillins. Although natural occurring penicillins are known to be good antibiotic, modern penicillins are semisynthetic compounds, structurally altered, through chemical modifications, to obtain products with improved biological activities and specific desirable properties. Currently there are many drugs produced by fungi, like the immunosuppressant ciclisporin (commonly used during transplant surgery), the fusidic acid, whose main application is the bacterial infection control and the statins, used to inhibit cholesterol synthesis. The use of fungi-derived compounds is a promising application field, and in the next few years, many new products with cardiovascular, anticancer, antiviral, Gas Blender 100 Series Application Note

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface A) Products naturally expressed by fungi are extracted and refined to produce drugs. B) Fungi cultures as new syrthetic route, used to replace complicated and expensive steps. Lab Culture Selected Fungi antibacterial, antiparasitic, anti-inflammatory, and antidiabetic properties are supposed to be refined to become globally available. Aside from the production of antibiotics and drugs, fungi are also used for the expression of various enzymes particularly useful for industrial processes. Moreover fungi act efficiently...

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface Increasing the CO2 concentration in the culture atmosphere usually involves a growth-suppression effect, detrimental for drugs expression [4,5] but useful for shelf-life enhancement [6]. The combination of low oxygen (>0,5%) and high CO2 (>15%) is a condition used both to suppress [7,8] and to enhance [9] fungi proliferation, while high oxygen high CO2 atmospheres are less common but still promising [10]. Gas Blending: common solution The standard gas blending solution used for culture application consists in 3 single...

High Performance Gas flow Dilutor & Gas flow Mixing System with User Interface References [1] Wen-Xian Zhang and Jian-Jiang Zhong, Effect of oxygen concentration in gas phase on sporulation and individual ganoderic acids accumulation in liquid static culture of Ganoderma lucidum – J Biosci Bioeng 109, 1 (2010) 37-40. [2] William R. Kenealy and Diane M. Dietrich, Growth and fermentation responses of Phanerochaete chrysosporium to O2 limitation – Enzyme Microb Technol 34, 2 (2004) 490-498. [3] S. Samapundo, B. De Meulenaer, A. Atukwase, J. Debevere and F. Devlieghere, The influence of modified...