Mix gases to create and control hypoxic environments

Product catalog summary

Project Overview: The project, in collaboration with the University of Maryland Baltimore, aims to grow strictly anaerobic microbes under microaerobic conditions. The head of project management is C. S. Raman, and MCQ Instruments plays a crucial role in creating and controlling specific hypoxic environments.

University Background: The University of Maryland, Baltimore, established in 1807, is a public health, law, and human services university. It is home to the University of Maryland BioPark, a major biotechnology cluster that supports the commercialization of new drugs, treatments, and devices.

Project Details: The project focuses on anaerobic microbes, micro-flow, and hypoxic atmospheres. It aims to achieve cost savings by reducing gas consumption by 30% and time savings through easier setup management of hardware and software. The GB100 Series allows precise control of flow rates from 0.1 ml/min to 500 ml/min without cut-off, ensuring flow stability and automation of experiments.

Scientific Context: The project addresses the challenge of culturing obligately anaerobic organisms in the presence of 0.01 - 1% O2. Recent studies have shown that even strict anaerobes cannot completely escape oxygen and must develop mechanisms to cope with it. The project seeks to uncover energy conservation mechanisms in microbes, particularly focusing on sulfate-reducing bacteria (SRB) and their ability to mediate O2 respiration and couple it to ATP production.

Research Significance: The research challenges the classical view of SRB as obligate anaerobes, demonstrating their ability to thrive in oxic environments and potentially conserve energy through O2 reductases. The project aims to establish laboratory conditions for successful culturing of anaerobic organisms in low oxygen environments.

Catalog excerpts

Mix gases to create and control hypoxic environments-1

University of Maryland Baltimore 0 ANAEROBIC MICROBES 0 MICRO-FLOW 0 HYPOXIC ATMOSPHERE A SOLID BUSINESS CASE IN COLLABORATION WITH THE “UNIVERSITY OF MARYLAND BALTIMORE “ TARGET OF THE PROJECT: Growing Strictly Anaerobic Microbes Under Microaerobic Conditions DEPARTMENT: University of Maryland Baltimore HEAD OF PROJECT MANAGEMENT: C. S. Raman ROLE OF MCQ INSTRUMENTS: To create and control, specific hypoxic environments. MORE INFORMATION ABOUT THE HEAD OF THE PROJECT Opened in 1807, the University of Maryland, Baltimore (UMB) is Maryland's public health, law, and human services university, dedicated to excellence in education, research, clinical care, and public service. The University of Maryland BioPark, Baltimore's biggest biotechnology cluster, fuels the commercialization of new drugs, treatments, and devices, giving 1,000 scientists and entrepreneurs the space to create and collaborate.

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Mix gases to create and control hypoxic environments-2

DESCRIPTION OF THE APPLICATION AND THE TARGET Very little is known about how microbes harvest energy for long term survival in their native niches. Laboratory-based assessments of extreme energy stress in organisms, such as methane producing Archaea, are unimpressive when compared to deep-sea sediment microbes, which support cellular respiration rates of 1 e¯ cell-1 sec-1. Surprisingly, recent studies have shown that oxygen (O2) penetrates up to 75 m below the sea oor, suggesting that achieving anaerobiosis in the biosphere is rather diﬃcult. That is, even the “strict” anaerobes cannot escape...

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