Compact pump-controlled heart perfusion system
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langendorfsystem Compact pump-controlled heart perfusion system

MDE LTD, A LEADING MEDICAL TECHNOLOGY COMPANY, HAS REVOLUTIONIZED THE FIELD OF CARDIOVASCULAR RESEARCH WITH ITS STATE-OF-THE-ART ISOLATED HEART PERFUSION SYSTEMS. THESE ADVANCED SYSTEMS OFFER RESEARCHERS AND PHYSICIANS A POWERFUL TOOL FOR STUDYING AND UNDERSTANDING THE INTRICACIES OF THE HEART. Isolated heart perfusion involves the process of temporarily disconnecting the heart from the body and perfusing it with a specialized solution that mimics the conditions of the human body. MDE Ltd’s perfusion systems provide a controlled environment for the heart, allowing researchers to investigate...

Two independent reservoirs with a total volume of 2 liters Special removable heart suspending unit MAP sensors of different sizes for mouse, rat, guinea pig and rabbit heart An electronically controlled flow speed to ensure the desired constant pressure (0-500 Hgmm) Parameters to examine: MAP, LVP, perfusion pressure, coronary flow, puffer temperature, heart rate, ECG, surface potential, muscle tone and muscle strength (even with force-displacement technique) The heart can be externally controlled (pacing), or stimulated Langendorff and Janicki-type working heart mode Built-in thermostat...

The system has to liquid reservoirs. Each reservoir can contain 1000ml saline, which provides a two litre liquid background for the experiment with the continuous rollback. The two reservoirs can be used independently. In this way the effects of the same compound can be tested in two different doses, or the effect of two different compounds in succession. The substance quantity required for the experiment can be significantly reduced with the small dead volume, which also substantially reduces costs (e.g.: in case of especially expensive or exotic compounds). A tap placed on the closing cap...

The suspension of the heart and the sensor connection is simplified by the fact that the cap with four point leads and spring-load, closing the liquid tank is removable, so the heart suspending unit can be removed from the heart chamber area. The tools necessary for the experiment are fixed to the closing cap. • heart suspender, • stimulator, MAP sensor holder • manipulator, • tap regulating the saline, • balloon catheters of the • Janicki-type working heart unit, • material inlet channel, • temperature sensor, • bubble trap We develop a heart suspender, where the dissection can be placed...

The taps are essential parts of the system, which lead the saline to the preparation. For this purpose we designed a multiposition, high-quality and longwearing tap. The essence of the construction is that the material combination of the tap allows appropriate grip surface, gapless sealing, and allows smooth closing/ opening movement. Moreover, contrary to other systems’ taps, it does not have two positions (open/ close), but it closes continuously. 1 Buffer tank 3 Peristaltic pump 2 Filling up tap (left and right side) 4 Buffer reservoires (lest and right side) 5 Elastic chamber 6 Selector...

The heart suspending module lifts up semiautomatically, then the whole unit sinks into the 12-lead MAP sensor after heart was fastened to it. Due to this design heart suspension is very easy because there is a lot of space for hands. The MAP sensor can be adjusted to the proper position for adequate electrical connections. There are different sized MAP sensors for mouse, rat, guinea-pig and rabbit hearts. The flow of perfusates is driven by a Gilson-like peristaltic pump equipped with 8 rollers. Flow rate is electronically adjusted to keep the desired pressure (between 0-500 mmHg) at every...

For the appropriate operation of the heart, appropriate quantity and quality carbogen gas is necessary being dissolved in the saline solution. For the necessary dispersion we developed a gas vaporizer and fine tuning device. The gas flowing through the redactor of the bottle, is collected in a windbag, and through a cylindrical clamp – a tube with appropriate wall thickness - the carbogen gas is vaporized into the solution. We deliver the system in a complete setup, which contains all the sensors and amplifiers necessary for pressure measurement and the measurement of MAP signals and the...

APPLICATION The animal is anesthetized and heparinized. The animal heart is removed from the chest cavity and suspended on perfusion cannula of Langendorff apparatus via aorta. The aortic root is tied. The coronaries are perfused with carbogenized, pH stabilized buffer solution. Temperature of the buffer is maintained by water-jacketed glassware. Two modes of perfusion can be carried out: constant pressure by hydrostatic pressure or constant flow mode by roller pump. Both methods provides retrograde perfusion when the aortic valve is permanently closed, so the buffer flows through the...

ADVANTAGES OF THE ISOLATED HEART PERFUSION DISADVANTAGES OF THE ISOLATED HEART PERFUSION Relatively easy to perform an experiment on intact organ Cardiac specific effects can be investigated Due to the artificial circumstances, less physiological than an in vivo method Used in biochemical, physiological, morphological, Following stabilization period (about 20-30 min) parameters are deteriorating continuously pharmacological studies, including drug screening • Experiment duration not longer than 3 to 4 hours The controlled environment (buffer, oxygen, pH, Certain parameters cannot be...

The advent of transgenic technology will undoubtedly result in increasing numbers of studies using murine preparations and this will require investigators to become adept at using this, as yet, poorly characterised model. Unfortunately, although the literature contains an increasing number of studies which employ mouse hearts, the fundamental characteristics of the preparation (e.g. pressurevolume and calcium-function relationships) have yet to be completely characterised. Another caution relates to the high heart rate of the mouse and the miniaturisation of equipment which requires...