tissuebath IS Modular isolated tissue bath systems for muscle strip or vessel ring preparation

MDE LTD, A LEADING COMPANY IN THE MEDICAL DEVICE INDUSTRY, SPECIALIZES IN THE PRODUCTION OF INNOVATIVE TISSUE BATHES. WITH A STRONG COMMITMENT TO ADVANCING HEALTHCARE AND ENHANCING RESEARCH CAPABILITIES, MDE LTD HAS BECOME SYNONYMOUS WITH EXCELLENCE IN TISSUE BATH TECHNOLOGY. Tissue bathes, developed by MDE Ltd, are meticulously designed systems that provide an ideal environment for the study of various biological tissues. These bathes serve as controlled platforms where researchers can immerse tissue samples in a carefully regulated medium, allowing for precise experimentation and analysis....

MICROTENSIONER AIDED PRETENSION ADJUSTMENT BY ADJUSTING THE HOLDER LENGTH, THE CHAMBER VOLUME CAN BE SET TO THE EXACT DESIRED VOLUME. THE MODULAR SETUP’S FEATURES OF TISSUE BATHS BY MDE. THE TISSUE HOLDER IS DESIGNED TO ENABLE THE APPLICATION OF TISSUE ON THE HOLDER OUTSIDE OF THE CHAMBER. THUS, IT ALLOWS EASY AND HANDY OPERATION. Easy upgrade to 2, 4, 8 channels Replaceable tissue baths (5, 10, 20 ml calibrated volume) Special ad-on vertical tissue bath with extra small, 1 ml calibrated volume Tissue holders with built in electrodes for point or field stimulation and with gas bubbler SIMILARLY,...

Force transducer Drug administration Carbogen in An isolated tissue bath is a chamber in which isolated organs or tissues can be administered with drugs or stimulated electrically, in order to measure their function. The tissue in the organ bath is typically oxygenated with carbogen and kept in a solution such as Krebs-Henseleit, Tyrode or lactated Ringer solution. Kreb’s solution out (overflow) Sample holder Stimulator electrode Stimulator electrode Tissue 37 °C water circulation Carbogen pipe Measuring area filled with Kreb’s solution Emptying 37 °C water circulation in It is used in pharmacology/physiology...

THEORICAL SETUP To keep the tissue sample alive and to perform a successful measurement, we need: • A pre-warmed and sufficiently saturated buffer before the measurement. This is provided by a double-walled reservoir (B) with an oxygenator placed in the system. • Buffer with optimum volume and temperature in the measuring vessel. The measuring bath (A) is also double-walled. The measuring bath is equipped with an overflow outlet to ensure that the calibrated amount of buffer always remains in the measuring chamber after washing out. • The system is kept warm (37°C) by a circulating water bath...

THE MECHANICAL SETUP © Base plate with stainless still tray (2 Measuring bath and movable bath clamp with teflon sample holder Microtensioner with force measuring sensor (4 Double walled buffer reservoir © Lower horizontal stiffening element (6 Upper horizontal stiffening element (7 Vertical holder bars

BUFFER RESERVOIR, TISSUE BATH, SENSOR HOLDER, GAS SUPPLIER WITH ADJUSTER Buffer reservoir, tissue bath, sensor holder and gas supplier with adjuster are the main components of one complete unit. These are assembled on the stand as one unit, then the tubing connections is done. This structure makes the replacement of any parts easy in case of damage. Moreover, malfunction of any part in the unit does not influence the operation of other units in the system, which is a further advantage. THE TISSUE BATH (TISSUE BATH) The measuring bath is the most complex component of the IS system. The double-walled...

In the latest redesign, the unit has been fitted with redesigned measuring bath fixings (3,4). The measuring chamber can now be lowered, making it much easier to place the sample on the sample holder. PARTS OF THE MEASURING BATH Carbogen inlet to bubbler Water jacket inlet Emptying tap Carbogen gas distribution outlet to the next bath Carbogen inlet Water jacket outlet to pressure adjuster Buffer overflow Buffer inlet Carbogen tubing connection to bubbler 10 Carbogen pressure adjuster tap 11 Emptying outlet

The measuring bath is filled with buffer from the reservoir through the B1 - B2 tube operated by filling tap (T1, 8). The tissue bath has two outlets. The upper outlet is an overflow (2) to keep the calibrated volume. The lower outlet (11) is for emptying the tissue chamber during operation or during cleaning. The unit keeps the flow of the water in a closed circuit providing stable temperature in the water jacket of the tissue chamber and the buffer reservoir as well. The inlet (4) supplies the chamber with Carbogen. Pressure adjustment of the gas can be done by the adjuster (10). Measuring...

THE TISSUE HOLDERS HAS FOUR MAIN FUNCTIONS • Setting of tissue bath volume by volume displacement with 0.1% accuracy Providing carbogen into the buffer by a built in gas bubbler Providing electrical stimulation of the tissue sample by built in stimulating electrodes Providing a centralized suspension of the tissue sample Teflon holder body Connector of stimulator electrodes Oxygenation metallic tube with atomizer Stimulator electrodes Connection to carbogen tube An easy-to-use accessory holder can be used to attach larger blood vessels (from Ø0,8mm), intestines or similar organ parts to the holder....

ISO-08-6A 1ML OR 2ML EXTREME SMALL VOLUME BATH The extreme small volume bath offer a highly controlled microenvironment that allows researchers to closely monitor and manipulate the conditions surrounding the muscle. The small volume minimizes the need for excessive reagents, making it cost-effective and reducing potential interference with the experimental setup.The limited volume helps in maintaining a stable concentration of solutions, solutions, such as various ions or neurotransmitters, which directly impact muscle contraction. This stability is vital for accurate and consistent measurements...

ISO-08-6E SPECIAL HOLDER FOR DIAPHRAGM - PHRENIC NERVE PREPARATION CONDUCTANTIA STUDIES The phrenic nerve-diaphragm preparation holds immense value in isolated muscle contraction experiments due to its unique physiological characteristics and relevance in studying neuromuscular function. This preparation involves the connection of the phrenic nerve, which innervates the diaphragm muscle responsible for breathing, to an isolated muscle setup. Its utility stems from several key aspects: Representative of Respiratory Function: The di- aphragm is the primary muscle involved in breathing. Studying...