whole organ perfusion system In vitro measuring setup for isolated liver and kidney perfusion experiments

whole organ perfusion system ADVANTAGES OF ISOLATED ORGAN PERFUSION SYSTEM: • The system maintains the isolated organ function in an evaporation chamber lying horizontally. It means that the whole isolated organ is not mounted, there fore its tions, e.g. hepatic metabolism, bile secretion, urine se- vasculature is not deformed by an artificial position. cretion out of the body. The organ preparations are Additionally, the organ is not immersed in a buffer liq- not endangered by thrombosis or embolism, or any uid thus extra hydrostatic pressure does not increase cardiovascular failure of the experimental...

whole organ perfusion system Control temperature sensor Control temperature sensor fields of application In case of kidney preparation cannulas sized 23G should be inserted into the ureter, renal vein and renal artery, and then the kidney can be taken out. The cannulas should be fitted to the appropriate parts of the perfusion system and the kidney can be placed into the perfusion chamber. In case of liver preparation polyethylene cannula size 18G should be inserted into the portal vein, then inferior vena cava is tied below the liver. The chest should be opened and another 18G cannula should...

whole organ perfusion system INTRODUCTION OF THE BATH The aim of the unique design of the of the organ bath is to keep the perfused organs alive as long as it possible. Thus valuable metabolites and biomarkers can be released with the help of contamination free perfusion solution for further analyses. The novelty of the technical design is that the organ is not kept in the physiological solution in order to survive, but in a 35.40C carbogen rich moisture vapor made from physiological solution. To ensure that the correct physiological conditions two hermetically separated chambers were made within...

whole organ perfusion system TEMPERATURE SENSORS - CONNECTING THE SENSORS There are 2 separate temperature (CHl and CH2) controller unit. Each temperature controller has 2 sensors and controls one heating element in the specific part of the tank. • CH1 temperature controller controls the moisture around organ CH2 temperature controller controls the temperature of the perfusion liquid in the silicon pipe The sensors and heaters are connected via the combinated cable to the temperate controller

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