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Ampli1™ LowPass Kit

Product catalog summary

Overview: The document discusses the Ampli1™ LowPass Kit, a tool for genome-wide profiling of copy-number alterations (CNA) in single cells, particularly useful for analyzing genomic heterogeneity in tumor cell populations such as circulating tumor cells (CTCs).

Specifications: The Ampli1™ LowPass Kit is a rapid, high-throughput low-pass Whole Genome Sequencing (WGS) kit designed for Copy Number Alteration analysis on single cells. It is compatible with Ion Torrent™ platforms and provides results in approximately 6 hours.

Features:

Accurate characterization of CNA profiles for multiple single tumor cells per run.
Fast library preparation with a 6-hour turnaround time.
Cost-effective for massively parallel single-cell analysis.

Methodology: The kit utilizes the deterministic nature of the Ampli1™ WGA Kit to provide precise snapshots of cancer cell genomes, reducing bench work and costs. Its accuracy has been validated by comparing its results with those from the array CGH method.

Applications: The kit is suitable for profiling single CTCs and white blood cells (WBCs) from cancer patients, providing insights into genomic alterations. A representative example includes whole genome profiles of a single CTC and a single WBC isolated from a lung adenocarcinoma patient.

Process: The workflow involves pure cell blood collection, CTC enrichment and staining, DEPArray sorting, whole genome amplification, low-pass WGS library preparation, molecular characterization, and sequencing.

Conclusion: The Ampli1™ LowPass Kit offers a scalable, rapid, and affordable method for single-cell copy-number profiling, making it a valuable tool for cancer research and genomic studies.

Catalog excerpts

EVERY CELL HAS A STORY TO TELL:FAST SINGLE CELL COPY NUMBER PROFILING m MENARINI ^ ^ silicon biosystems^^ www.siliconbiosystems.com Genome-wide profiling of copy-number alterations (CNA) in single cells enables the characterization and investigation of genomic heterogeneity and evolution in tumor cell populations such as individual circulating tumor cells (CTCs). Ampli1™ LowPass Kit is a rapid, high-throughput low-pass Whole Genome Sequencing (WGS) Kit for Copy Number Alteration analysis on single cells. Get a High Quality Single Cell Library in 6 Hours!

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LOW-PASS WGS LIBRARY PREP KIT FOR ION TORRENT™ PLATFORMS FAST SINGLE CELL COPY NUMBER PROFILING Blood Collection CTCs Enrichment & Staining DEPArray Sorting 100% Pure Cell Whole Genome Amplification Low-Pass WGS Library Prep Molecular Characterization Sequencing By exploiting the deterministic nature of the Ampli1™ WGA Kit, the Ampli1™ LowPass Kit provides a precise snapshot of cancer cell genome, drastically reducing the amount of bench work and cost. The accuracy of this method has been assessed by comparing the copy number profiles generated by our approach with those obtained by the array...

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