Reamplify the Ampli1™ WGA product obtained from single cells or from batch of cells, in a reliable and reproducible way. The reamplification will provide further WGA-amplified DNA from the same cell; for downstream genetic analysis use the Ampli1™ Reamplification product exactly as Ampli1™ WGA product; Convert ssDNA molecules into dsDNA molecules both in Ampli1™ WGA products and in Ampli1™ Reamplification products, and increase the total dsDNA content. This procedure is recommended for accurate DNA quantitation, high-throughput high dimensional downstream analysis and NGS applications in general, ensuring higher sequencing uniformity. A notable increase of Ampli1™ WGA’ed DNA is achieved through ssDNA to dsDNA conversion Ampli1™ dsDNA Synthesis Effect on Single Cell Whole Genome Amplified DNA dsDNA [ng] in 25 ul of purified sample Assessment of dsDNA quantity among n=15 single cells, comprised TCs and WBCs (fresh, PFA2%-fixed and CellSearch®-fixed ) from Ampli1™ WGA Kit, shows that the dsDNA synthesis allows a relevant increase (up to 6X) of the dsDNA content of Ampli1™ WGA products in short time (30 minutes); Similarly, for n=6 Ampli1™ WGA reamplification products from 2 single TCs (3 technical replicates of ReAmp per sample) a good increase of dsDNA content is reported following 30’ dsDNA synthesis (up to 3,5X). The Ampli1™ ReAmp/ds Kit ensures the conversion of ssDNA molecules into dsDNA molecules in Ampli1™ WGA/ReAmp products population in a quick and reproducible way, yielding amounts of single cell WGA’ed dsDNA suitable

Ampli 1ReAmp/ds Kit Amplil™ single cell WGA products are reamplified while maintaining genetic representation Ampli1™ Reamplification Effect on Single Cell STR Profile ,U J T T J J J vO T T T T T T For n=67 alleles detected with Amp/i1™ STR Kit in Amp/i1™ WGA products from n=4 single cells, comprised of 1 TC and 3 WBCs from different treatments (fresh; PFA2%-fixed; CellSearch®-fixed) 100% of alleles were redetected after WGA reamplification among 3 technical replicates per sample. Single cell 1 Single cell 2 Single cell 3 Single cell 4 WBC WBC WBC TC The Amp//1™ ReAmp/ds Kit provides...