Balanced, robust whole genome amplification from single cells V MENARINI | M silicon biosystems

Ampli1™ WGA Kit balanced allelic amplification Every Cell Has A Story To Tell™ The Ampli1™ Whole Genome Amplification (WGA) Kit has been developed and optimized to deliver a balanced and complete amplification of the total DNA content of a single cell. 1 When working with pure, single cells, achieving balanced amplification of both alleles at every single locus is very challenging. On a whole genome sequencing test 2, The Ampli1™ WGA Kit has demonstrated a significantly lower ADO (allelic drop out) rate (2%) as compared with Multiple Displacement Amplification (MDA) (up to 65%). In addition, the percentage...

• RELIABLE: Single primer-mediated PCR ensures balanced amplification • REPRODUCIBLE: Single tube, no-precipitation protocol minimizes template loss • ROBUST: Works on fresh and fixed single cells silicon biosystems

DNA may be derived from fresh, fixed and/or stained cells, including FFPE derived tumor and stromal cells. 3,4,5,6 Additionally, hundreds of positive experiments have been executed on single cells isolated from multiple kinds of samples: WBCs, CTCs, sperm cells, CSF, stem cells, neural cells and fetal cells. Furthermore, results obtained with the Ampli1™ WGA Kit did not reveal any significant decrease of WGA quality on cells fixed and/or stored for a long time. 7 Mean SCR % on Single Cells Ampli1™ WGA Kit is ideal for Various Cell Types 100% Robust amplification from fixed or live cells STR Call Rates...

Reliable sequencing of DNA from single cells after amplification with AmplH™ WGA Kit 8 Single tumor and white blood cells (WBCs) were isolated from controls spiked with tumor cells and cancer patient samples using Menarini Silicon Biosystems’ DEPArray™ system. Genomic DNA from each individual tumor cell and WBC were amplified using the Amplil™ WGA Kit and then sequenced with the Ion Torrent® Amp//Seq™ Custom Panel. The sequence table below shows the detection of homozygous (HM) and heterozygous (HT) gene mutations. The results demonstrate very low allelic drop out (ADO), no false positive calls,...

Maximum Flexibility for Downstream AnalysesProduct Information Description Amplified DNA generated with the Amp//1TM WGA Kit can be used for the most demanding laboratory workflow and genomic applications and is suitable with the following methods and analysis: Description • Amp//1TM WGA Kit and Amp/i1TM QC Kit are available in 50 rxn and 200 rxn respectively. Methods • Sanger Sequencing • aCGH • qPCR • Next Generation Sequencing « Analysis • Sequencing • SNP and Mutation Detection • STR • CNV • Loss of Heterozygosity Amp//TM Sequencing Kits Menarini Silicon Biosystems has developed Sanger ^...

Ampin™ QC Kit (Genome Integrity Index) The AmpiH™ QC Kit provides a useful quality control check of the WGA procedure by utilizing a PCR-based assay to establish DNA integrity. By testing for the presence of one short and three long DNA fragments in the WGA library, the quality of the DNA as starting material for downstream analyses can be assured. Cells with good quality DNA typically produce 3 or 4 PCR bands, while fixation of cells or cells with degraded DNA will show fewer QC bands. It has been proved that the number of the bands revealed is useful to define a “Genome Integrity Index” for...

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