Digitally sorting 100% pure cells

DEPArray™ NxT, the new benchtop instrument for fully automated identification and digital isolation of rare single-cells, with 100% purity, allows for unprecedented precision in the molecular characterization of sorted cells. DEPArray™ sorting joins the robustness of fluorescent IHC markers with the power of bright-field imaging for accurate cell identification and leverages the precision of semiconductor CMOS technology for exact cell isolation. Find the cell you are looking for and get it 100% pure. CellBrowser™ software elaborates fluorescence and bright-field images enabling automatic or...

Highly advanced cartridge Cartridge DEPArray™ Cartridge combines microfluidics and microelectronics for an unprecedented precision in manipulating single-cells. An array of micro electrodes, controlled by a semiconductor chip, covers the floor of the flow cell and allows the generation of up to 30.000 “DEP cages”, each one able to capture a cell through a dielectrophoretic force. After microscope observation and selection, the desired cells are moved to a collection tube by simple stepwise motion of the positive DEP cages by automated software. Software The CellBrowser™ software enables automatic or...

Precise cell identification based on high-definition images Recovery Area Parking Area The DEPArrayTM NxT optical system produces high-resolution pictures of the content of each DEP cage, enabling the use of sophisticated proprietary image analysis algorithms, capable of automatic cell identification based on the operator’s preset parameters. Main chamber Schematic representation of the DEPArrayTM flow cell. The sample is loaded in the main chamber, the selected cells are moved first to the parking area and from there, single or in pools, into the recovery area for delivery into a collection...

DEPArray^xT High-resolution optical system The new optical system collects high-resolution images directly on the first scan, allowing a higher detection rate for rare events and higher confidence in the selection. Real-time, multichannel image composition in false color distinguishes the cell populations during the chip scan. Simultaneous chip scan allows you to immediately visualize the output during the scan.

High level of automation Automatic cell selection for FFPE samples Automatic identification of tumor and stromal populations Image analysis algorithm automatically enables cell selection in less than 1 second by following these 3 steps: Immediately after the scan, the software automatically identifies the tumor/stromal population based on multiparametric high content imaging. tumor / stromal population identification ploidy selection fine cell selection Manual selection > 30-60 min Automatic selection < 1 s Scatterplot based on fluorescence intensity (CK/Vimentin)

DEPArray^xT Automatic ploidy selection Ploidy selection is based on DAPI integral intensity peak detection which is an important parameter correlated with DNA content. The position of the DAPI intensity peak precisely defines the absolute ploidy of each cell population. In the last step, the system performs fine selection based on 4 parameters: • Quality of staining • Specificity of staining • Cell size • Individual cell vs cluster The two cell populations are ready to be routed and recovered. The pictures of each individual cell contained in the pools are stored and can be visualized in a panel...

Delivering 100% pure cells, live and fixed The DEPArrayTM system is able to work with both fixed cells and live cells and has a minimum recommended input of only 300 cells. • 96 recoveries of single cells • Extremely gentle with live cells • Up to 600 pure sorted cells can be delivered in pools from a single

AUTOMATIC REPORT CTC sample throughput The 1st cell can be obtained in about 1 hour. Each additional cell recovery takes about 80 seconds. AUTOMATIC REPORT

User friendly Graphical user interface The completely redesigned graphical user interface is intuitive and simple. BARCODE READER FOR UNAMBIGUOUS IDENTIFICATION OF: • Operator • Sample • Cartridge • Recovery Su

Chip scan, image analysis & CellBrowser™ real-time synchronization The system allows you to synchronize the chip scan with the image analysis and CellBrowserTM. The data obtained during the image analysis phase is now available in real-time and can be visualized in the CellBrowserTM during the chip scan. Cell routing can begin at any time during your experiment, even before the chip scan has been completed. Chip scan & analysis Data available in the CellBrowser™ in real-time, during the chip s

User friendly Advanced unit control • Remote / centralized control of multiple units (up to 5 units) • Post Processing, review experiment data • Synchronized, automatic, real-time data back-up + Remote multitask control workst

Automatic report generation At the end of each run, data are organized automatically in a report, making all relevant information about the experiment, from set up to results, available at a glance. All relevant information is collected in the report and can be easily retrieved

CTC application Liquid biopsy CTCs are important mediators of metastasis, and their relevance for cancer prognosis, monitoring and prediction to therapy guidance is becoming increasingly more recognized CTCs are extremely rare in comparison to hematologic cells, which makes them difficult to isolate. DEPArrayTM digital sorting enables the collection of 100% pure CTC from blood enriched with various methods, including EpCAM positive selection (CellSearch), size selection, negative depletion by beads or FACS. Validated workflows will drive you through the processing of CTC DNA for mutation detection...

The future of tumor stratification: Copy Number Aberrations of single CTCs Delivering 100% pure single CTCs, DEPArrayTM eliminates the noise determined by contaminating WBC and allows very precise and high-resolution Copy Number Aberration profiling of each single cell. Ampli1TM WGA with Ampli1TM Low Pass Genome kit workflow is a robust and easy-to-use solution to deliver reliable profiling of tumor genome aberration. Low-pass Whole Genome Profile (chr 1-22) of single CTC (upper panel) and WBC (lower panel) isolated with DEPArrayTM NxT from a patient with Lung Adenocarcinoma. NGS libraries were...