FFPE Application Digital revolution, sharp resolution Resolving heterogeneity in solid tumors w MENARINI j silicon biosystems

FFPE Application FFPE analysis beyond your expectations Accurate analysis of tumor molecular profile is essential for effective implementation of precision medicine in oncology. Currently, heterogeneity of solid tumor specimens, together with lack of consistency in tumor cellularity between samples, represents a significant limitation to precise quantitative analysis of tumor genetic characteristics. Indeed deep sequencing analysis of FFPE samples only describes the relative abundance of each variant in the bulk of DNA molecules generated by nucleic acid extraction from the sample. • The newly...

DEPArray™ TECHNOLOGY Make every sample relevant Deep molecular investigation technologies like NGS, digital PCR and digital microarrays enhance your ability to analyze heterogeneous samples but still cannot overcome limitations due to poor representation of targets, dilution of quantitative markers, lack of standardization in sample preparation from FFPE blocks. The use of DEPArray™ technology on disaggregated FFPE tissue samples enables separation and collection of distinct cell populations, making it possible to: • Analyze each cell population in purity • Precisely know which cell population...

Digital resolution: PRECISION Because researchers continue to experience difficulties when using traditional thechnology to process consistently all their FFPE samples, we now provide a technology that can universally allow for separation and recovery of pure cell populations from any FFPE sample. The DEPArray™ FFPE application can overcome recurrent issues associated with conventional techniques.

DEPArray™ TECHNOLOGY PRECISION: delivered The DEPArray™ system can be used to separate tumor, stromal and other subpopulations from heterogeneously mixed cells in suspension derived from a disaggregated FFPE section. Admixture of stromal and tumour DNA Incomplete Interpretation of NGS results De-paraffinization, Lysis, Heating FFPE Sample De-paraffinization, dissociation & Ab labeling Sorting of 100% pure cell population Cell Lysis PRECISE and reliable understanding of cancer genetics

Resolving sample heterogeneity Heterogenous Cluster Tumor cells Only DEPArray™ technology enables 100% purity in cell separation due to: • Combination of fluorescent and bright field imaging, to distinguish individual cell from cell aggregates • Digitally controlled cell recovery, to ensure highest purity The suspension of cells obtained from FFPE samples is stained with fluorescent markers for specific cell types (e.g. Cytokeratin and Vimentin) and DAPI for nuclear staining. Stained cells are loaded onto the DEPArray™ cartridge where 100 % pure cells can be digitally isolated from each cell...

DEPArray™ TECHNOLOGY Reproducible data, precise results Variant report table from sequencing analysis of 5 libraries obtained from an Ovarian Cancer FFPE sample. An Ovarian Cancer FFPE slice was analyzed by NGS using Ion Torrent AmpliSeq™ Cancer Hotspot Panel V.2 as an unsorted FFPE sample and as 3 distinct cell populations identified as follow: • Stromal: Vim+/Ck- normal DNA content cells. • Cancer: Vim-/Ck+ High DNA content cells. • Double Positive: Vim+/Ck+ High DNA content cells. The displayed table shows some variants found in at least one group of cells. Numbers in the chart indicate variant...

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