ChromBook
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ChromBook
Product catalog summary
Introduction
Merck Millipore, established in 1904, is a leader in liquid chromatography, known for its innovative products and techniques. The company focuses on sorbents, analytical and preparative columns, sample preparation, thin layer chromatography, and biochromatography products, emphasizing quality and customer-focused solutions.
Merck Millipore, established in 1904, is a leader in liquid chromatography, known for its innovative products and techniques. The company focuses on sorbents, analytical and preparative columns, sample preparation, thin layer chromatography, and biochromatography products, emphasizing quality and customer-focused solutions.
Mobile Phases & Reagents
Merck Millipore offers high-purity HPLC solvents like LiChrosolv® and Prepsolv®, which are essential for accurate chromatographic results due to their low UV absorbance, low particle count, and low acidity and alkalinity.
Merck Millipore offers high-purity HPLC solvents like LiChrosolv® and Prepsolv®, which are essential for accurate chromatographic results due to their low UV absorbance, low particle count, and low acidity and alkalinity.
Sample Preparation
The company provides a range of sample preparation products, including EXtrelut® for liquid-liquid extraction and LiChrolut® for solid-phase extraction, designed for efficient and reliable sample preparation.
The company provides a range of sample preparation products, including EXtrelut® for liquid-liquid extraction and LiChrolut® for solid-phase extraction, designed for efficient and reliable sample preparation.
Thin Layer Chromatography (TLC)
Merck Millipore offers a variety of TLC and HPTLC products, including classical silica plates and high-performance silica plates, catering to a wide range of applications.
Merck Millipore offers a variety of TLC and HPTLC products, including classical silica plates and high-performance silica plates, catering to a wide range of applications.
Analytical HPLC
The company provides a wide range of HPLC columns, including Chromolith®, Purospher®, and LiChrospher®, suitable for various applications, including LC/MS and UHPLC.
The company provides a wide range of HPLC columns, including Chromolith®, Purospher®, and LiChrospher®, suitable for various applications, including LC/MS and UHPLC.
Preparative HPLC
Merck Millipore offers standardized sorbents and pre-packed columns for preparative chromatography, ensuring high method reliability and optimized throughput.
Merck Millipore offers standardized sorbents and pre-packed columns for preparative chromatography, ensuring high method reliability and optimized throughput.
Ion Chromatography
The SeQuant® SAMS membrane suppressor and CARS continuous regeneration system enhance detectability and simplify analysis in suppressed ion chromatography.
The SeQuant® SAMS membrane suppressor and CARS continuous regeneration system enhance detectability and simplify analysis in suppressed ion chromatography.
Gas Chromatography
The company supplies high-purity solvents like SupraSolv® and UniSolv® for gas chromatography, along with sorbents, derivatization reagents, and standards for accurate and reproducible results.
The company supplies high-purity solvents like SupraSolv® and UniSolv® for gas chromatography, along with sorbents, derivatization reagents, and standards for accurate and reproducible results.
Conclusion
Merck Millipore's ChromBook provides detailed insights into their chromatography products and applications, guiding users through the diverse world of chromatography with a focus on quality and innovation.
Merck Millipore's ChromBook provides detailed insights into their chromatography products and applications, guiding users through the diverse world of chromatography with a focus on quality and innovation.
Solvent Quality and Applications
Prepsolv® solvents are tailored for scale-up from analytical to preparative separations, offering low residue on evaporation for optimal product yield and column protection. The Solvent Management System ensures safe and contamination-free solvent usage.
Prepsolv® solvents are tailored for scale-up from analytical to preparative separations, offering low residue on evaporation for optimal product yield and column protection. The Solvent Management System ensures safe and contamination-free solvent usage.
Lab Water Purification Systems
Merck Millipore systems produce ultrapure water, crucial for reducing water contaminants prior to liquid chromatography.
Merck Millipore systems produce ultrapure water, crucial for reducing water contaminants prior to liquid chromatography.
LiChrosolv® Solvents
These solvents are available in various grades and packaging, suitable for both isocratic and gradient methods in modern analytical HPLC.
These solvents are available in various grades and packaging, suitable for both isocratic and gradient methods in modern analytical HPLC.
Specifications and Ordering Information
Detailed specifications for various solvents, including acetonitrile and methanol, are provided, highlighting their purity and other critical parameters.
Detailed specifications for various solvents, including acetonitrile and methanol, are provided, highlighting their purity and other critical parameters.
LiChrosolv® Hypergrade Solvents
These solvents set a new standard for HPLC solvent quality, enabling analysis in low ppb trace ranges and ensuring high UV-transmittance and baseline stability in LC-MS applications.
These solvents set a new standard for HPLC solvent quality, enabling analysis in low ppb trace ranges and ensuring high UV-transmittance and baseline stability in LC-MS applications.
Prepsolv® Solvents
Designed for preparative chromatography, these solvents have extremely low evaporation residue and water content, facilitating scale-up from analytical to preparative scale.
Designed for preparative chromatography, these solvents have extremely low evaporation residue and water content, facilitating scale-up from analytical to preparative scale.
Safe Solvent Handling
Merck Millipore emphasizes user safety and reliability in chromatographic results, offering a range of solvent handling technologies and accessories.
Merck Millipore emphasizes user safety and reliability in chromatographic results, offering a range of solvent handling technologies and accessories.
Ultrapure Water Production
The document outlines the process of producing ultrapure water suitable for HPLC, involving pretreatment and polishing steps.
The document outlines the process of producing ultrapure water suitable for HPLC, involving pretreatment and polishing steps.
Sample Preparation
Techniques include off-line methods like liquid-liquid extraction with EXtrelut® and solid-phase extraction with LiChrolut®.
Techniques include off-line methods like liquid-liquid extraction with EXtrelut® and solid-phase extraction with LiChrolut®.
Key Products and Ordering Information
Detailed product listings for water purification kits, ion pair reagents, and sample preparation materials are provided with ordering numbers.
Detailed product listings for water purification kits, ion pair reagents, and sample preparation materials are provided with ordering numbers.
EXtrelut® NT Extraction Columns
EXtrelut® NT columns are used for efficient liquid-liquid extraction, overcoming the disadvantages of classical extraction methods.
EXtrelut® NT columns are used for efficient liquid-liquid extraction, overcoming the disadvantages of classical extraction methods.
LiChrolut® Solid-Phase Extraction
LiChrolut® products are designed for selective extraction of components from complex samples, offering benefits such as time and solvent savings.
LiChrolut® products are designed for selective extraction of components from complex samples, offering benefits such as time and solvent savings.
LiChrolut® EN Specifications
Material: Ethyl vinyl benzene divinyl benzene polymer. Particle Size: 40 - 120 µm. Specific Surface: 1,200 m²/g (BET method).
Material: Ethyl vinyl benzene divinyl benzene polymer. Particle Size: 40 - 120 µm. Specific Surface: 1,200 m²/g (BET method).
Benefits of LiChrolut® EN
High adsorption capacity for polar organic substances, use of common solvents and buffers across the pH range, reduced solvent usage, and time savings.
High adsorption capacity for polar organic substances, use of common solvents and buffers across the pH range, reduced solvent usage, and time savings.
LiChrospher® ADS Specifications
Sorbent Type: Spherical silica gel with dual surface modifications. Particle Size: 25 µm. Pore Diameter: 60 Å.
Sorbent Type: Spherical silica gel with dual surface modifications. Particle Size: 25 µm. Pore Diameter: 60 Å.
Benefits of LiChrospher® ADS
Direct extraction and enrichment of hydrophobic, low molecular weight analytes, designed for HPLC analysis of biological samples.
Direct extraction and enrichment of hydrophobic, low molecular weight analytes, designed for HPLC analysis of biological samples.
LiChrospher® ADS System
The sample is injected onto a precolumn, which retains analytes while unwanted components are flushed out.
The sample is injected onto a precolumn, which retains analytes while unwanted components are flushed out.
Ion Exchangers
Includes gel, macroporous, fluid ion exchangers, and adsorber resins, each with specific applications.
Includes gel, macroporous, fluid ion exchangers, and adsorber resins, each with specific applications.
Millex® Syringe Filters
Essential for high-quality separation and purification processes in various scientific fields.
Essential for high-quality separation and purification processes in various scientific fields.
Samplicity™ Filtration System Overview
The Samplicity™ Filtration System is designed to enhance sample preparation efficiency by outperforming manual filtration methods.
The Samplicity™ Filtration System is designed to enhance sample preparation efficiency by outperforming manual filtration methods.
Thin Layer Chromatography (TLC) Overview
Merck Millipore offers a range of TLC solutions for fast and versatile separation of substances.
Merck Millipore offers a range of TLC solutions for fast and versatile separation of substances.
Specifications and Applications
TLC Plates: Suitable for in-process control of drugs, purity checking, and identity testing of pharmaceutical compounds.
TLC Plates: Suitable for in-process control of drugs, purity checking, and identity testing of pharmaceutical compounds.
Key Features
HPTLC plates have a mean particle size of 5-6 µm, offering faster analysis times and higher sensitivity.
HPTLC plates have a mean particle size of 5-6 µm, offering faster analysis times and higher sensitivity.
RP-Modified Silica Plates
These plates are suitable for analyzing basic or acidic substances, with good separation of compounds like gallic acid and its esters.
These plates are suitable for analyzing basic or acidic substances, with good separation of compounds like gallic acid and its esters.
Cellulose TLC and HPTLC
Cellulose plates are used for separating polar substances such as amino acids and carbohydrates.
Cellulose plates are used for separating polar substances such as amino acids and carbohydrates.
ProteoChrom® HPTLC Plates
Designed for peptide analysis, these plates offer highly efficient separations and are stable in aqueous solvent systems.
Designed for peptide analysis, these plates offer highly efficient separations and are stable in aqueous solvent systems.
Technical Appendix Overview
This document provides detailed information on thin layer chromatography (TLC) and analytical high-performance liquid chromatography (HPLC) techniques.
This document provides detailed information on thin layer chromatography (TLC) and analytical high-performance liquid chromatography (HPLC) techniques.
Analytical HPLC
HPLC is essential for the separation, quantitation, and identification of compounds in various industries.
HPLC is essential for the separation, quantitation, and identification of compounds in various industries.
Method Development
The document outlines a structured approach to HPLC method development, emphasizing the importance of defining method goals, understanding sample characteristics, and optimizing selectivity and system parameters.
The document outlines a structured approach to HPLC method development, emphasizing the importance of defining method goals, understanding sample characteristics, and optimizing selectivity and system parameters.
Conclusion
The document serves as a comprehensive guide for professionals using TLC and HPLC, providing insights into best practices, equipment selection, and method optimization to ensure high-quality analytical results.
The document serves as a comprehensive guide for professionals using TLC and HPLC, providing insights into best practices, equipment selection, and method optimization to ensure high-quality analytical results.
HPLC Method and System Selection
The process begins with selecting the appropriate HPLC method and system, considering the sample as the central focus.
The process begins with selecting the appropriate HPLC method and system, considering the sample as the central focus.
Sample Preparation Procedure
Establishing a sample preparation procedure is crucial for effective HPLC analysis.
Establishing a sample preparation procedure is crucial for effective HPLC analysis.
Detector Selection
The choice of detector depends on several factors, including the chemical nature of analytes, potential interferences, and required sensitivity.
The choice of detector depends on several factors, including the chemical nature of analytes, potential interferences, and required sensitivity.
Initial Conditions Selection
Selecting initial conditions involves choosing the mode of separation, column, and mobile phase.
Selecting initial conditions involves choosing the mode of separation, column, and mobile phase.
Preliminary Separations and Optimization
Preliminary separations help in understanding the behavior of analytes under initial conditions.
Preliminary separations help in understanding the behavior of analytes under initial conditions.
Method Validation
The final step is method validation, ensuring that the developed method meets the required standards for accuracy, precision, and sensitivity.
The final step is method validation, ensuring that the developed method meets the required standards for accuracy, precision, and sensitivity.
Key Considerations
The document emphasizes the importance of keeping the method simple and focusing on significant factors for achieving desired resolution.
The document emphasizes the importance of keeping the method simple and focusing on significant factors for achieving desired resolution.
Column Selection
The document emphasizes the importance of choosing the right column format to achieve high efficiency and resolution.
The document emphasizes the importance of choosing the right column format to achieve high efficiency and resolution.
Particle and Pore Size
Smaller particle sizes offer higher separation efficiency, while larger sizes allow faster flow rates and are less prone to clogging.
Smaller particle sizes offer higher separation efficiency, while larger sizes allow faster flow rates and are less prone to clogging.
Carbon Load and Endcapping
Carbon load affects the hydrophobicity and retention times of compounds.
Carbon load affects the hydrophobicity and retention times of compounds.
Mobile Phase Selection
The document outlines the properties of solvents and buffers, highlighting the differences between isocratic and gradient elution.
The document outlines the properties of solvents and buffers, highlighting the differences between isocratic and gradient elution.
Isocratic vs. Gradient Elution
Isocratic elution is suitable for samples with a narrow range of polarities, while gradient elution is recommended for complex mixtures.
Isocratic elution is suitable for samples with a narrow range of polarities, while gradient elution is recommended for complex mixtures.
Gradient Method Development
Recommendations include avoiding gradients from 100% aqueous to 100% organic and maintaining a minimum percentage of each phase in mobile phase bottles.
Recommendations include avoiding gradients from 100% aqueous to 100% organic and maintaining a minimum percentage of each phase in mobile phase bottles.
System Optimization
After achieving satisfactory selectivity, parameters such as column dimension, particle size, and flow rate should be optimized.
After achieving satisfactory selectivity, parameters such as column dimension, particle size, and flow rate should be optimized.
Conclusion
The document concludes with a step-by-step guide for method development, from selecting the HPLC method and system to method validation, emphasizing the importance of each step in achieving optimal results.
The document concludes with a step-by-step guide for method development, from selecting the HPLC method and system to method validation, emphasizing the importance of each step in achieving optimal results.
Method Specifications
The document outlines a specific HPLC method using phosphoric acid and acetonitrile with a gradient composition.
The document outlines a specific HPLC method using phosphoric acid and acetonitrile with a gradient composition.
Method Validation
Validation is crucial for ensuring consistent results over time and across different laboratories.
Validation is crucial for ensuring consistent results over time and across different laboratories.
Accuracy and Precision
Accuracy is defined as the closeness of measurements to the actual value, often expressed in terms of standard error.
Accuracy is defined as the closeness of measurements to the actual value, often expressed in terms of standard error.
Specificity and Detection Limits
Specificity ensures the method can accurately measure the analyte in the presence of other components.
Specificity ensures the method can accurately measure the analyte in the presence of other components.
Linearity and Robustness
Linearity refers to the method's ability to produce results proportional to analyte concentration.
Linearity refers to the method's ability to produce results proportional to analyte concentration.
Column Selection Guide
The document provides a guide for selecting HPLC columns based on the chemical structure of analytes, stationary phase, sorbent specifications, and column dimensions.
The document provides a guide for selecting HPLC columns based on the chemical structure of analytes, stationary phase, sorbent specifications, and column dimensions.
Conclusion
The document serves as a detailed resource for developing and validating HPLC methods, ensuring reliable and accurate analytical results in pharmaceutical and chemical analysis.
The document serves as a detailed resource for developing and validating HPLC methods, ensuring reliable and accurate analytical results in pharmaceutical and chemical analysis.
Specifications of Silica Particles
Type A and Type B Particles: These are conventional and high-purity spherical silica particles, respectively, with modifications such as Octyl, Octadecyl, and amino bonding.
Type A and Type B Particles: These are conventional and high-purity spherical silica particles, respectively, with modifications such as Octyl, Octadecyl, and amino bonding.
Applications
Reversed-Phase Chromatography: Utilizes hydrophobic interactions for separating medium to less polar compounds.
Reversed-Phase Chromatography: Utilizes hydrophobic interactions for separating medium to less polar compounds.
Column Selection Guide
Selection Criteria: Based on chemical structure, stationary phase, sorbent specifications, column dimensions, and USP classification.
Selection Criteria: Based on chemical structure, stationary phase, sorbent specifications, column dimensions, and USP classification.
Chromolith® HPLC Columns
Monolithic Silica Technology: Offers faster analysis and lower operating pressure compared to traditional particulate columns.
Monolithic Silica Technology: Offers faster analysis and lower operating pressure compared to traditional particulate columns.
Key Benefits
Speed and Efficiency: Chromolith® columns provide up to four times faster analysis than 5µm particulate columns.
Speed and Efficiency: Chromolith® columns provide up to four times faster analysis than 5µm particulate columns.
Chromolith® CapRod®
Chromolith® CapRod® is a monolithic capillary column designed for high throughput and sensitive proteomics-LC applications.
Chromolith® CapRod® is a monolithic capillary column designed for high throughput and sensitive proteomics-LC applications.
Specifications
The columns are made from monolithic silica gel with inner diameters of 50 µm, 100 µm, and 200 µm, and lengths of 150 mm and 300 mm.
The columns are made from monolithic silica gel with inner diameters of 50 µm, 100 µm, and 200 µm, and lengths of 150 mm and 300 mm.
Performance and Advantages
Chromolith® CapRod® columns provide excellent separation efficiency with reduced analysis time.
Chromolith® CapRod® columns provide excellent separation efficiency with reduced analysis time.
Applications
These columns are suitable for separating small molecules, peptides, and proteins, and are compatible with various Nano or Capillary LC systems.
These columns are suitable for separating small molecules, peptides, and proteins, and are compatible with various Nano or Capillary LC systems.
Ordering Information
The document provides detailed ordering information for various Chromolith® CapRod® products, including dimensions and contents of each package.
The document provides detailed ordering information for various Chromolith® CapRod® products, including dimensions and contents of each package.
Recommended Use and Flow Rates
The document outlines recommended uses for different column types and provides flow rate ranges and maximum back-pressure specifications.
The document outlines recommended uses for different column types and provides flow rate ranges and maximum back-pressure specifications.
Separation Examples
Examples of separations using Chromolith® CapRod® columns are provided, demonstrating their efficiency and repeatability in separating biological compounds and small molecules.
Examples of separations using Chromolith® CapRod® columns are provided, demonstrating their efficiency and repeatability in separating biological compounds and small molecules.
Benefits
Chromolith® HPLC columns offer faster analysis, improved system security, and cost savings due to increased sample throughput.
Chromolith® HPLC columns offer faster analysis, improved system security, and cost savings due to increased sample throughput.
Chromolith® RP-18 and RP-8 endcapped columns
The Chromolith® RP-18 and RP-8 endcapped columns are designed for reversed-phase chromatography.
The Chromolith® RP-18 and RP-8 endcapped columns are designed for reversed-phase chromatography.
Procedures
The document outlines the use of Chromolith® columns in various HPLC setups, detailing mobile phases, flow rates, pressures, and detection methods.
The document outlines the use of Chromolith® columns in various HPLC setups, detailing mobile phases, flow rates, pressures, and detection methods.
Performance and Benefits
Chromolith® columns offer ultra-fast results with low back-pressure, making them suitable for both UHPLC and standard HPLC systems.
Chromolith® columns offer ultra-fast results with low back-pressure, making them suitable for both UHPLC and standard HPLC systems.
Applications
The document includes examples of separations achieved using Chromolith® columns, such as the separation of antihistamines, carotenoids, and bioflavonoids.
The document includes examples of separations achieved using Chromolith® columns, such as the separation of antihistamines, carotenoids, and bioflavonoids.
Recommendations
Chromolith® columns are recommended for applications requiring high efficiency and fast separations.
Chromolith® columns are recommended for applications requiring high efficiency and fast separations.
Chromolith® NH2 columns
Chromolith® NH2 columns are made from highly porous monolithic silica rods with a bimodal pore structure.
Chromolith® NH2 columns are made from highly porous monolithic silica rods with a bimodal pore structure.
Applications
Chromolith® columns are used in analytical HPLC for separating complex mixtures.
Chromolith® columns are used in analytical HPLC for separating complex mixtures.
Guard Cartridges
To enhance the longevity and performance of Chromolith® columns, guard cartridges are available.
To enhance the longevity and performance of Chromolith® columns, guard cartridges are available.
Column Couplers
Chromolith® column couplers allow for the coupling of multiple columns to increase separation efficiency.
Chromolith® column couplers allow for the coupling of multiple columns to increase separation efficiency.
SemiPrep Columns
Chromolith® SemiPrep columns offer a direct scale-up from analytical to preparative LC.
Chromolith® SemiPrep columns offer a direct scale-up from analytical to preparative LC.
Ordering Information
The document includes detailed ordering information for various Chromolith® products, including performance columns, guard cartridges, and column couplers, with specific product numbers and dimensions.
The document includes detailed ordering information for various Chromolith® products, including performance columns, guard cartridges, and column couplers, with specific product numbers and dimensions.
Chromolith® Performance RP-18 endcapped columns
Chromolith® Performance RP-18 endcapped columns are noted for their fast separation capabilities.
Chromolith® Performance RP-18 endcapped columns are noted for their fast separation capabilities.
Procedures
Scale-up from analytical to preparative columns involves linear transfer of methods, with adjustments in flow rate and injection volume based on column dimensions.
Scale-up from analytical to preparative columns involves linear transfer of methods, with adjustments in flow rate and injection volume based on column dimensions.
Norms and Recommendations
Chromolith® Prep columns are recommended for high-speed separations in preparative chromatography, offering better selectivity and reduced separation times.
Chromolith® Prep columns are recommended for high-speed separations in preparative chromatography, offering better selectivity and reduced separation times.
Key Data and Findings
Chromolith® Prep columns can operate at flow rates up to 400 mL/min, significantly higher than particulate columns.
Chromolith® Prep columns can operate at flow rates up to 400 mL/min, significantly higher than particulate columns.
Customer Benefits
Low pressure drop at higher flow rates and fast adsorption/desorption kinetics.
Low pressure drop at higher flow rates and fast adsorption/desorption kinetics.
Purospher® high-purity HPLC columns
Purospher® columns are characterized by high purity silica with polymeric C18 modification and endcapping.
Purospher® columns are characterized by high purity silica with polymeric C18 modification and endcapping.
Chromatographic Properties
The document highlights the use of Tanaka and Engelhardt tests to evaluate the chromatographic properties of Purospher® columns.
The document highlights the use of Tanaka and Engelhardt tests to evaluate the chromatographic properties of Purospher® columns.
Applications
Purospher® STAR RP-18 endcapped columns are versatile, suitable for separating a wide range of compounds with simple mobile phases.
Purospher® STAR RP-18 endcapped columns are versatile, suitable for separating a wide range of compounds with simple mobile phases.
Ordering Information
The document includes detailed ordering information for various Purospher® STAR RP-18 endcapped column configurations, including different lengths and internal diameters.
The document includes detailed ordering information for various Purospher® STAR RP-18 endcapped column configurations, including different lengths and internal diameters.
Product Specifications
Purospher® STAR RP-18 endcapped columns are available in various dimensions and particle sizes, primarily 3 µm and 5 µm.
Purospher® STAR RP-18 endcapped columns are available in various dimensions and particle sizes, primarily 3 µm and 5 µm.
Surface Modification and Characterization
The columns feature a polymeric surface modification that provides nearly perfect surface coverage, preventing polar interactions.
The columns feature a polymeric surface modification that provides nearly perfect surface coverage, preventing polar interactions.
Purity and Performance
The columns are made from high-purity silica, ensuring tailing-free chromatography for acidic, basic, and chelating compounds.
The columns are made from high-purity silica, ensuring tailing-free chromatography for acidic, basic, and chelating compounds.
pH Stability
Purospher® STAR RP-18 endcapped columns exhibit outstanding pH stability, remaining stable and reproducible across a pH range of 1.5 to 10.5.
Purospher® STAR RP-18 endcapped columns exhibit outstanding pH stability, remaining stable and reproducible across a pH range of 1.5 to 10.5.
Tanaka Test Results
The Tanaka test is used to compare the selectivity and performance of HPLC columns.
The Tanaka test is used to compare the selectivity and performance of HPLC columns.
Use with Aqueous Phases
These columns can be used with 100% aqueous mobile phases without suffering from phase collapse.
These columns can be used with 100% aqueous mobile phases without suffering from phase collapse.
Stability and Efficiency
The columns maintain efficiency and retention time over extended use, with stability tests confirming their robustness over 15,000 column volumes.
The columns maintain efficiency and retention time over extended use, with stability tests confirming their robustness over 15,000 column volumes.
Separation Examples
Various separation examples are provided, demonstrating the columns' effectiveness in separating compounds such as triptylines, catecholamines, sweeteners, hormones, and peptides.
Various separation examples are provided, demonstrating the columns' effectiveness in separating compounds such as triptylines, catecholamines, sweeteners, hormones, and peptides.
LC/MS Applications
To address ghost peaks in LC/MS applications, a three-step procedure is recommended.
To address ghost peaks in LC/MS applications, a three-step procedure is recommended.
Purospher® STAR HPLC columns
The Purospher® STAR RP-18 and RP-8 endcapped columns are based on high-purity silica with endcapping, offering excellent peak symmetry and stability across a wide pH range (1.5 to 10.5).
The Purospher® STAR RP-18 and RP-8 endcapped columns are based on high-purity silica with endcapping, offering excellent peak symmetry and stability across a wide pH range (1.5 to 10.5).
Applications
These columns are suitable for high-throughput screening, QC analyses, process monitoring, and method development.
These columns are suitable for high-throughput screening, QC analyses, process monitoring, and method development.
Performance
The document emphasizes the columns' ability to deliver tailing-free chromatograms, high efficiency, and excellent selectivity for both acidic and basic compounds.
The document emphasizes the columns' ability to deliver tailing-free chromatograms, high efficiency, and excellent selectivity for both acidic and basic compounds.
Ordering Information
Detailed ordering information is provided for various column configurations, including product numbers, particle sizes, dimensions, and package contents.
Detailed ordering information is provided for various column configurations, including product numbers, particle sizes, dimensions, and package contents.
Conclusion
Purospher® STAR columns offer a versatile solution for a wide range of chromatographic applications, providing high performance, stability, and efficiency.
Purospher® STAR columns offer a versatile solution for a wide range of chromatographic applications, providing high performance, stability, and efficiency.
LiChrospher® and Superspher® product lines
The document outlines the characteristics of LiChrospher® packing materials, which are spherical silica carriers available in various modifications.
The document outlines the characteristics of LiChrospher® packing materials, which are spherical silica carriers available in various modifications.
Procedures and Applications
The document provides examples of pharmaceutical and environmental analyses using LiChrospher® 100 RP-18 columns.
The document provides examples of pharmaceutical and environmental analyses using LiChrospher® 100 RP-18 columns.
Recommendations
For optimal performance, the document recommends using specific cartridge holders for LiChroCART® columns and suggests compatible guard columns.
For optimal performance, the document recommends using specific cartridge holders for LiChroCART® columns and suggests compatible guard columns.
Ordering Information
Detailed ordering information is provided for various LiChrospher® and Superspher® columns, including product numbers, particle sizes, dimensions, and package contents.
Detailed ordering information is provided for various LiChrospher® and Superspher® columns, including product numbers, particle sizes, dimensions, and package contents.
Key Data from Tables
The tables in the document present critical data such as specific surface area, pore volume, and carbon load for different LiChrospher® materials.
The tables in the document present critical data such as specific surface area, pore volume, and carbon load for different LiChrospher® materials.
LiChrospher® 100 RP-18
Used for high-resolution separations of peptides and tRNA molecules.
Used for high-resolution separations of peptides and tRNA molecules.
LiChrospher® PAH
Designed for high-resolution separation of polycyclic aromatic hydrocarbons (PAH).
Designed for high-resolution separation of polycyclic aromatic hydrocarbons (PAH).
LiChrospher® 100 RP-8 and RP-8 Endcapped
Suitable for chromatography of acidic, neutral, and weakly basic compounds.
Suitable for chromatography of acidic, neutral, and weakly basic compounds.
LiChrospher® 60 RP-select B
Optimized for the determination of basic substances with excellent properties for neutral and acidic substances.
Optimized for the determination of basic substances with excellent properties for neutral and acidic substances.
Separation Examples
Various separation examples are provided for each column type, demonstrating their application in different analytical scenarios.
Various separation examples are provided for each column type, demonstrating their application in different analytical scenarios.
Ordering Information
Comprehensive ordering details for each column type, including product numbers, dimensions, and package contents.
Comprehensive ordering details for each column type, including product numbers, dimensions, and package contents.
LiChrospher® 60 RP-select B
Available in different particle sizes (5 µm and 10 µm) and packaging options.
Available in different particle sizes (5 µm and 10 µm) and packaging options.
LiChrospher® 100 CN
Features both polar and hydrophobic properties, suitable for complex samples.
Features both polar and hydrophobic properties, suitable for complex samples.
LiChrospher® 100 NH2
Versatile sorbent for reversed-phase, normal phase, and ion exchange chromatography.
Versatile sorbent for reversed-phase, normal phase, and ion exchange chromatography.
LiChrospher® 100 DIOL
Suitable for complex samples and exclusion chromatography.
Suitable for complex samples and exclusion chromatography.
LiChrospher® Si 60 and Si 100
Used for normal phase chromatography with polar properties.
Used for normal phase chromatography with polar properties.
LiChrosorb®
Irregular shaped silica sorbent used in HPLC for over 25 years.
Irregular shaped silica sorbent used in HPLC for over 25 years.
Key Specifications
Particle sizes range from 5 µm to 10 µm.
Particle sizes range from 5 µm to 10 µm.
Applications
The document includes examples of environmental analysis and separation of complex samples using different LiChrospher® sorbents.
The document includes examples of environmental analysis and separation of complex samples using different LiChrospher® sorbents.
LiChrosorb® Packing Materials
LiChrosorb® RP-8 and RP-18 are silica-based materials with octyl and octadecyl derivatives, respectively.
LiChrosorb® RP-8 and RP-18 are silica-based materials with octyl and octadecyl derivatives, respectively.
Aluspher® 100 RP-select B
Designed for alkaline stable HPLC separations, using alumina particles coated with polybutadiene.
Designed for alkaline stable HPLC separations, using alumina particles coated with polybutadiene.
SeQuant® ZIC®-HILIC and ZIC®-pHILIC
These columns are ideal for separating polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC) mode.
These columns are ideal for separating polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC) mode.
Conclusion
The document provides comprehensive information on the specifications, applications, and ordering details for various HPLC columns and packing materials.
The document provides comprehensive information on the specifications, applications, and ordering details for various HPLC columns and packing materials.
ZIC®-HILIC and ZIC®-pHILIC columns
The document lists multiple ZIC®-HILIC columns with varying dimensions and particle sizes, suitable for different analytical needs.
The document lists multiple ZIC®-HILIC columns with varying dimensions and particle sizes, suitable for different analytical needs.
Applications and Procedures
The document provides examples of separations using ZIC®-HILIC columns, such as the separation of cyanuric acid and melamine.
The document provides examples of separations using ZIC®-HILIC columns, such as the separation of cyanuric acid and melamine.
Chiral Separations
The document discusses the importance of chiral separations in pharmaceuticals and other industries.
The document discusses the importance of chiral separations in pharmaceuticals and other industries.
Ordering Information
Detailed ordering information is provided for various columns, including product numbers, particle sizes, dimensions, and package contents.
Detailed ordering information is provided for various columns, including product numbers, particle sizes, dimensions, and package contents.
Conclusion
The document serves as a comprehensive guide for selecting and using ZIC®-HILIC and ZIC®-pHILIC columns in HPLC applications.
The document serves as a comprehensive guide for selecting and using ZIC®-HILIC and ZIC®-pHILIC columns in HPLC applications.
LiChroCART® Cartridges
Available in various lengths (75, 125, 150, 250 mm) and internal diameters (2, 3, 4, 4.6 mm).
Available in various lengths (75, 125, 150, 250 mm) and internal diameters (2, 3, 4, 4.6 mm).
Hibar® Columns
Available in lengths of 30, 125, 250 mm and internal diameters of 3, 4, 4.6, 10 mm.
Available in lengths of 30, 125, 250 mm and internal diameters of 3, 4, 4.6, 10 mm.
EcoCART® Glass Cartridges
Require a specific glass cartridge holder for use.
Require a specific glass cartridge holder for use.
Nucleosil® Sorbent Cartridges
Available in various particle sizes and dimensions.
Available in various particle sizes and dimensions.
Sorbents and Packing Materials
Includes a variety of sorbents like Purospher®, Superspher®, LiChrosorb®, and LiChrospher®.
Includes a variety of sorbents like Purospher®, Superspher®, LiChrosorb®, and LiChrospher®.
Accessories
manu-CART® cartridge holders are reusable and fit various cartridge lengths and diameters.
manu-CART® cartridge holders are reusable and fit various cartridge lengths and diameters.
Recommendations
Use of guard columns is recommended for extending the life of the main column.
Use of guard columns is recommended for extending the life of the main column.
Conclusion
The document provides comprehensive details on the specifications, ordering, and usage of HPLC columns and accessories, emphasizing ease of use and cost-effectiveness.
The document provides comprehensive details on the specifications, ordering, and usage of HPLC columns and accessories, emphasizing ease of use and cost-effectiveness.
Column Specifications and Hardware
The document outlines various types of HPLC columns, including Hibar®, LiChroCART®, Chromolith®, and SeQuant®, each with specific pressure limits and solvent restrictions.
The document outlines various types of HPLC columns, including Hibar®, LiChroCART®, Chromolith®, and SeQuant®, each with specific pressure limits and solvent restrictions.
Column Installation and Care
Proper installation is crucial to avoid dead volumes that reduce separation efficiency.
Proper installation is crucial to avoid dead volumes that reduce separation efficiency.
Column Equilibration
Equilibration ensures consistent results.
Equilibration ensures consistent results.
Column Performance Validation
Each column comes with a test certificate for separation efficiency and selectivity.
Each column comes with a test certificate for separation efficiency and selectivity.
Mobile Phases and Solvent Compatibility
Silica-based columns are compatible with various organic solvents within specified pH ranges.
Silica-based columns are compatible with various organic solvents within specified pH ranges.
Column Lifetime and Stability
Column lifetime depends on sample and conditions.
Column lifetime depends on sample and conditions.
Storage and Regeneration
Short-term storage can be in the eluent, while long-term storage requires specific solvents like acetonitrile.
Short-term storage can be in the eluent, while long-term storage requires specific solvents like acetonitrile.
Troubleshooting
Common issues include changing retention times, broad peaks, and ghost peaks.
Common issues include changing retention times, broad peaks, and ghost peaks.
Troubleshooting Guide for Analytical HPLC
Poor Sample Recovery: Issues such as absorption or adsorption of proteins can be mitigated by changing the HPLC mode or adding solubilizing agents.
Poor Sample Recovery: Issues such as absorption or adsorption of proteins can be mitigated by changing the HPLC mode or adding solubilizing agents.
Leaks
Leaks at various points such as columns, detectors, and pumps can be addressed by checking and replacing fittings, seals, and rotors as necessary.
Leaks at various points such as columns, detectors, and pumps can be addressed by checking and replacing fittings, seals, and rotors as necessary.
Selectivity Issues
Differences in selectivity may arise from variations in mobile phase composition or solvent strength.
Differences in selectivity may arise from variations in mobile phase composition or solvent strength.
Baseline Disturbances
Problems like air bubbles or temperature changes can cause baseline disturbances.
Problems like air bubbles or temperature changes can cause baseline disturbances.
Pressure Problems
High back-pressure can be caused by blockages or inappropriate solvent use.
High back-pressure can be caused by blockages or inappropriate solvent use.
Preparative HPLC
Merck Millipore provides standardized silica gel packing materials for preparative chromatography, ensuring high purity and regulatory compliance.
Merck Millipore provides standardized silica gel packing materials for preparative chromatography, ensuring high purity and regulatory compliance.
Key Data
The document includes tables detailing the characteristics of aluminium oxide packing materials and their typical technical data.
The document includes tables detailing the characteristics of aluminium oxide packing materials and their typical technical data.
Silica Gels
The document lists standardized silica gels with details on particle size, distribution, and content.
The document lists standardized silica gels with details on particle size, distribution, and content.
LiChroprep® Packing Materials
LiChroprep® is described as a reliable packing material for HPLC, known for its homogeneous silica gel matrix and narrow particle size distribution.
LiChroprep® is described as a reliable packing material for HPLC, known for its homogeneous silica gel matrix and narrow particle size distribution.
LiChrospher® Packing Materials
LiChrospher® is a spherical silica gel used in preparative HPLC.
LiChrospher® is a spherical silica gel used in preparative HPLC.
Silica 60 Silanized
This section discusses silanized silica gel as an economical alternative for product purification.
This section discusses silanized silica gel as an economical alternative for product purification.
Florisil®
Florisil® is a magnesium silicate used for separating steroids, alkaloids, and antibiotics.
Florisil® is a magnesium silicate used for separating steroids, alkaloids, and antibiotics.
Micro-crystalline Cellulose
This section covers cellulose as a packing material for the separation of amino acids and bio-molecules.
This section covers cellulose as a packing material for the separation of amino acids and bio-molecules.
Chromolith® Prep
Chromolith® Prep columns are highlighted for their speed, efficiency, and productivity in separations.
Chromolith® Prep columns are highlighted for their speed, efficiency, and productivity in separations.
Hibar® Pre-packed Columns
Hibar® columns are described as ready-to-use columns for transferring optimized separation methods.
Hibar® columns are described as ready-to-use columns for transferring optimized separation methods.
Conclusion
The document provides comprehensive information on various chromatography packing materials, their specifications, applications, and ordering details.
The document provides comprehensive information on various chromatography packing materials, their specifications, applications, and ordering details.
Scaling the Separation
Key parameters such as flow rate, tubing inner diameter, detector cell volume, and injection volume must be adjusted to prevent extra-column effects.
Key parameters such as flow rate, tubing inner diameter, detector cell volume, and injection volume must be adjusted to prevent extra-column effects.
Down-scaling
Focuses on minimizing extra-column volumes to maintain separation power.
Focuses on minimizing extra-column volumes to maintain separation power.
Decreased Efficiency
Discusses the impact of void volume on separation efficiency and the importance of minimizing tubing length and diameter to reduce band-broadening.
Discusses the impact of void volume on separation efficiency and the importance of minimizing tubing length and diameter to reduce band-broadening.
Injection Volumes
Provides guidelines for suitable injection volumes based on column i.d. to avoid volume overload and maintain efficiency.
Provides guidelines for suitable injection volumes based on column i.d. to avoid volume overload and maintain efficiency.
Mass Overload
Explains how excessive sample mass can lead to non-Gaussian peaks and reduced separation efficiency.
Explains how excessive sample mass can lead to non-Gaussian peaks and reduced separation efficiency.
Scaling Column Length
Describes the benefits of adjusting column length for quicker separations or higher resolution.
Describes the benefits of adjusting column length for quicker separations or higher resolution.
Instrumental Influence
Highlights the need to adjust for system dwell volume and detector flow cell volumes when scaling down.
Highlights the need to adjust for system dwell volume and detector flow cell volumes when scaling down.
Up-scaling
Discusses the transition from analytical to preparative scale, emphasizing the need for scalable phases and the challenges of maintaining performance.
Discusses the transition from analytical to preparative scale, emphasizing the need for scalable phases and the challenges of maintaining performance.
Norms and Recommendations
Use semi-micro flow cells for columns with 2.1 mm i.d. or smaller, and standard flow cells for larger columns.
Use semi-micro flow cells for columns with 2.1 mm i.d. or smaller, and standard flow cells for larger columns.
Data and Calculations
Includes tables and figures illustrating flow rates for different column i.d., suitable injection volumes, and recommended mass loads.
Includes tables and figures illustrating flow rates for different column i.d., suitable injection volumes, and recommended mass loads.
Practical Examples of Scale Purification
Analytical and Preparative Scale Purification: This section provides examples of scaling analytical separations to larger scales.
Analytical and Preparative Scale Purification: This section provides examples of scaling analytical separations to larger scales.
LiChroprep® Application for Prostaglandins
The second example discusses a LiChroprep® application for Prostaglandins.
The second example discusses a LiChroprep® application for Prostaglandins.
Alternative Scaling – From TLC to Preparative HPLC
This section highlights the use of TLC as a quick scouting method to predict conditions for preparative HPLC runs.
This section highlights the use of TLC as a quick scouting method to predict conditions for preparative HPLC runs.
Ion Chromatography
Ion chromatography is crucial for verifying water and food safety.
Ion chromatography is crucial for verifying water and food safety.
SeQuant® SAMS & CARS Suppressor System
The CARS system optimizes eluent suppression with the SAMS suppressor, ensuring low background conductivity and high analyte sensitivity.
The CARS system optimizes eluent suppression with the SAMS suppressor, ensuring low background conductivity and high analyte sensitivity.
Gas Chromatography
Gas chromatography remains a widely used analytical technique across various fields.
Gas chromatography remains a widely used analytical technique across various fields.
Overview
This document provides detailed information on the use of high-purity solvents for gas chromatography (GC) and their applications in various analytical procedures.
This document provides detailed information on the use of high-purity solvents for gas chromatography (GC) and their applications in various analytical procedures.
Specifications
The document outlines the specifications for SupraSolv® and UniSolv® solvents, which are designed for different GC applications.
The document outlines the specifications for SupraSolv® and UniSolv® solvents, which are designed for different GC applications.
Applications
SupraSolv® solvents are recommended for analyzing medium to high boiling substances, such as pesticides and PCBs, in environmental samples.
SupraSolv® solvents are recommended for analyzing medium to high boiling substances, such as pesticides and PCBs, in environmental samples.
Product Information
The document provides detailed ordering information for a range of solvents, including their purity levels, packaging options, and specific applications.
The document provides detailed ordering information for a range of solvents, including their purity levels, packaging options, and specific applications.
Standards and Guidelines
The document references international guidelines, such as those from the ICH, Ph Eur, and USP, for the analysis of residual solvents in pharmaceuticals.
The document references international guidelines, such as those from the ICH, Ph Eur, and USP, for the analysis of residual solvents in pharmaceuticals.
Recommendations
For accurate GC analysis, the document recommends using solvents with the highest purity to avoid interference and ensure reliable results.
For accurate GC analysis, the document recommends using solvents with the highest purity to avoid interference and ensure reliable results.
Derivatization Reagents
Derivatization is essential for analyzing substances with low volatility or stability.
Derivatization is essential for analyzing substances with low volatility or stability.
Ordering Information
The document provides ordering details for various derivatization reagents, including package sizes and product numbers.
The document provides ordering details for various derivatization reagents, including package sizes and product numbers.
Reference Substances
Reference substances are used for identifying unknown compounds and as standards in quantitative analysis.
Reference substances are used for identifying unknown compounds and as standards in quantitative analysis.
Hydrocarbons and Fatty Acid Methyl Esters
The document lists hydrocarbons (C5 to C19) and fatty acid methyl esters, providing product numbers, assay percentages, empirical formulas, and packaging details.
The document lists hydrocarbons (C5 to C19) and fatty acid methyl esters, providing product numbers, assay percentages, empirical formulas, and packaging details.
Indices and Contacts
The document includes a subject index and contact information for Merck Millipore, facilitating easy navigation and further inquiries.
The document includes a subject index and contact information for Merck Millipore, facilitating easy navigation and further inquiries.
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Catalog excerpts
Your guide to a fascinating Merck Millipore is a division of jMERCK
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Leading the way for over a century. Our journey began in 1904 with the development of an aluminum oxide for adsorption chromatography. Over the decades, numerous products and techniques followed. Many set new milestones. Each was recognized as important progress in the field.
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This spirit of innovation still continues relentlessly. Today, Merck Millipore is among the leaders in the science of liquid chromatography and is committed to the further development of sorbents, analytical and preparative columns, sample preparation, thin layer chromatography and biochromatography products. Whether for research and development, quality control or purification purposes, Merck Millipore products are most widely used throughout the world. How did we get where we are today? Through uncompromising quality and a strong customer focus. From the very beginning, our highest priorities...
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ChromBook at a glance 01 Mobile Phases & Reagents Merck Millipore’s high purity HPLC-solvents LiChrosolv® and Prepsolv® ensure separation results of excellent quality and provide highest safety in solvents-handling by combination with specially developed packaging and withdrawal systems. Merck Millipore Lab Water Systems provide on demand the best water quality of the most widely used solvent in the laboratory: water. Merck Millipore offers a top quality portfolio with EXtrelut® for solid phase supported liquid-liquid extraction, LiChrolut® for solid-phase extraction, LiChrospher® ADS for on-line...
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www.merck-chemicals.com/chromatography Visit our website and browse e.g. • Applications • Technical information • Information about current chromatography topics • Education material and much, much more ...
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01 Mobile Phases & Reagents 12 www.merck-chemicals.com/mobile-phases-for-chromatography Mobile Phases & Reagents for HPLC and TLC Solvents for analytical chromatography LiChrosolv® hypergrade A new standard in HPLC solvents Solvents for preparative chromatography Solvent Management System Solvents handling in bottles Options for solvents handling in barrels Production of ultrapure water suitable for HPLC Elix® Advantage system Infinte water purification Milli-Q® Advantage system User-adapted ultrapure water Milli-Q® Integral system Integrated pure and ultrapure water LiChropur® reagents for analytical...
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Sample Preparation Millex® Syringe Filters Filter with confidence Millex® Syringe Filters Sample preparation for chromatography Millex® Syringe Filters Sample preparation for UHPLC Millex® Syringe Filters Automation-compatible filter Samplicity™ Filtration System Break free from routine sample fitration Millex Samplicity™ Filters Provide maximum versatility with highest quality. Samplicity™ Filtration System Now you can truly do more with less. 03 Thin Layer Chromatography 90 www.merck-chemicals.com/thin-layer-chromatography Thin Layer Chromatography Classical silica TLC plates (TLC) For versatile...
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04 Analytical HPLC www.merck-chemicals.com/analytical-hplc Analytical HPLC Method development & optimization Column selection guide Speed and performance based on revolutionary monolithic silica technology Chromolith® CapRod® Monolithic capillary Chromolith® HPLC columns Speed and performance in monolithic form Chromolith® RP-18 endcapped columns are the fastest C18 columns in the world. Chromolith® guard cartridges and cartridge kits Chromolith® column coupler Chromolith® SemiPrep Perfect scale-up from analytical to preparative LC Chromolith® Prep Chromolith® – increase in speed, efficiency...
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Analytical HPLC LiChrospher® 100 RP-8 and RP-8 endcapped For reproducible reversed phase separation Excellent separations even with basic compounds Excellent for complex samples with polar and hydrophobic characteristics A versatile sorbent for both reversed phase and normal phase chromatography Excellent for complex samples with polar and hydrophobic characteristics and for exclusion chromatography Irregular shaped silica sorbent A successful packing material from the start Alkaline stable HPLC separations A stable reversed phase stationary phase optimized for applications up to pH 12 SeQuant®...
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05 Preparative HPLC www.merck-chemicals.com/preparative-hplc Preparative HPLC Aluminium oxide Standardized silica gels Other sorbents Micro-crystalline cellulose Chromolith® Prep Chromolith® – increase in speed, efficiency and productivity Hibar® pre-packed columns Hibar® customized pre-packed columns SeQuant® SAMS & CARS suppressor system High sensitivity and low background in anion chromatography
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High purity solvents for gas chromatography Solvents for headspace gas chromatography Solvents for organic trace analysis Sorbents for packed columns Derivatization reagents Reference substances Subject index Index of ordering numbers Merck Millipore around the world Analytical Application Finder and more educational material
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Mobile Phases & Reagents First stop: Merck Millipore laboratories, Darmstadt, Germany. Here you are bound to find many steady hands and agile minds – busy producing breakthrough mobile phases and reagents, which will be used to analyze chemical compounds in everything from food to pharmaceuticals. The task involves selecting the best raw materials and performing countless purification steps. This obsession with quality is the reason mobile phases from Merck Millipore are found in every HPLC laboratory worldwide. From a city in southern central in Germany to the rest of the globe ...
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Mobile Phases & Reagents Contents Mobile Phases & Reagents for HPLC and TLC LiChrosolv® hypergrade Solvent Management System Solvents handling in bottles Options for solvents handling in barrels Elix® Advantage system Milli-Q® Advantage system Milli-Q® Integral system LiChropur® reagents for analytical HPLC Solvents for analytical chromatography A new standard in HPLC solvents Solvents for preparative chromatography Production of ultrapure water suitable for HPLC Infinte water purification User-adapted ultrapure water Integrated pure and ultrapure water
Open the catalog to page 14All Merck catalogs and technical brochures
Filters and Supporting Hardware48 Pages
Archived catalogs
Milli-Q HR 700012 Pages
SDS 5008 Pages
Milli-Q® Integral system22 Pages
Elix® Advantage System20 Pages
AFS® 8D and 16D Systems8 Pages
RiOs DI® Clinical4 Pages
Spectroquant® Prove5 Pages
Amnis® Imaging Flow Cytometers36 Pages
Provantage® Services2 Pages
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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.