MERCK MILLIPORE Protein purification and prepai High purit recovery fi better disc Merck Millipore is a business of

Introduction Today, researchers are challenged to create high quality protein samples for successful proteome analysis, often using cumbersome traditional sample preparation methods. With over 50 years of experience in developing protein sample preparation technologies, Merck Millipore is constantly innovating new tools to offer you rapid and efficient solutions that can be smoothly integrated into your workflow. Why spend your time on arduous sample preparation protocols when you can focus your efforts on exciting experiments? With the right pure protein, in the buffer you need, at the concentration...

Protein Extraction When purifying proteins for functional activity, or otherwise expose it to or structural studies, the first step is to degradative conditions. Merck disrupt the cells or tissue sample and Millipore’s complete range of reagents extract the relevant protein fraction. and enzymes for cell lysis and protein This step is critical because processing extraction provide you with an array methods that require harsh mechanical, of options so that you can put together chemical, or enzymatic treatments can the perfect extraction protocol for your affect the target protein’s integrity and...

1D PAGE - One-dimensional Polyacrylamide Gel Electrophoresis 2D PAGE ! Two-dimensional Polyacrylamide Gel Electrophoresis IEF !Isoelectric Focusing * ! Salt must be removed before IEF

BugBuster® Protein Extraction Kits and Reagents Simple extraction of soluble protein from E. coli without sonication Gently disrupt the cell wall of E. coli and liberate soluble proteins with BugBuster® Kits and Reagents. BugBuster® reagent provides a simple, rapid, low-cost alternative to mechanical methods such as French press or sonication for releasing expressed target proteins in preparation for purification or other applications. The proprietary formulation uses a detergent mix to perforate cell walls without denaturing soluble protein. Simply harvest cells by centrifugation and suspend...

BugBuster® Reagent X BugBuster® Plus X Benzonase® Nuclease BugBuster® Plus Lysonase™ Kit X BugBuster® Master Mix X Benzonase® rLysozyme™ Nuclease Solution Notes Flexibility to customize dilution and buffer composition PopCulture® Reagent X 2 separate vials for greater flexibility X X 2 separate vials for greater flexibility Buffer protects protein from the pH extremes produced in high density culture media, enabling extraction directly in medium. We offer a family of protein extraction reagents for gentle, efficient, non-mechanical extraction of soluble proteins from bacteria,...

Cell Lysis and Nucleic Acid Removal Enhancers Featured Products Benzonase® Nuclease Effectively reduce viscosity and remove nucleic acids from protein solutions Benzonase® Advantages Benzonase® Nuclease is a genetically engineered • Functional between pH 6 and 10, from 0 °C to 42 °C endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and has an exceptionally high specific activity. Benzonase® nuclease is an excellent choice for viscosity...

rLysozyme™ Solution Degrade bacterial cell walls with stabilized recombinant lysozyme rLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of E. coli. The enzyme catalyzes the hydrolysis of N acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme™ (1700 KU/mg) for E. coli lysis is 250 times greater than that of traditional Specific Activity (Units/g) chicken egg white lysozyme. rLysozyme™ is optimally Chicken Egg White Lysozyme rLysozyme™ enhance the efficiency of protein extraction with BugBuster® and PopCulture®...

Protein Extraction with ProteoExtract® Kits Featured Products ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) Reproducible extraction of subcellular proteomes from mammalian cells. Based on different solubilities of certain subcellular compartments, the S-PEK uses proprietary chemistries to • Subcellular redistribution assays to monitor protein yield four subproteome fractions which are enriched in cytosolic, membrane/organelle, nuclear, and cytoskeletal • Enzyme activity assays including reporter gene assays proteins. In the case of adherent cells, the procedure is performed directly...

ProteoExtract® Native Membrane Protein Extraction Kit (M-PEK) Isolation of native membrane proteins from mammalian cells and tissue. Extract proteins associated with cellular membranes with M-PEK. The extremely mild extraction conditions yield a 3-5 fold enrichment of integral membrane and membrane-associated proteins. The simple, two-step procedure enables processing of multiple samples in parallel. Extraction from tissues requires isolation of viable cells before proceeding with the extraction protocol. Applications for Extracted Membrane Proteins: • Enzyme activity assays, including reporter...

Protein Extraction with Inhibitors Featured Products Protease Inhibitor Cocktails Prevent protein degradation by proteases during extraction and purification Protease Inhibitor Advantages: Ensure the integrity of purified proteins by using • Consistent―High quality ensures reproducibility protease inhibitor cocktails and highly specific protease inhibitors. During protein expression and isolation, endogenous proteases rapidly begin to degrade protein samples, reducing the quality and quantity of protein samples required for characterization and analysis. By using the right combination of protease...

Phosphatase Inhibitor Cocktails Prevent protein dephosphorylation for cell signaling studies It is critical to preserve the phosphorylation state of proteins of interest during their extraction from cell and tissue lysates. To effect cell signaling, target proteins are phosphorylated by protein kinases that transfer a phosphate group to specific sites, typically at serine, threonine, or tyrosine residues. These phosphate groups can be removed by protein phosphatases, restoring the protein to its original dephosphorylated state. Using phosphatase inhibitors help reveal the signaling status inside...