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Genetic Sequencer DNBSEQ-G400* Strengthen your daily sequencing capability Flexible, stable, and well-qualified, offering more choices 2 Flow Cell Types 5 Sequencing Modes 7 Sequencing Lengths Leading Life Science Innovation
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About MGI MGI Tech Co., Ltd. (referred to as MGI) is committed to building core tools and technology to lead life science through intelligent innovation. MGI focuses on R&D, production and sales of DNA sequencing instruments, reagents, and related products to support life science research, agriculture, precision medicine and healthcare. MGI is a leading producer of clinical high-throughput gene sequencers, and its multi-omics platforms include genetic sequencing, mass spectrometry, medical imaging, and laboratory automation. As of December 31, 2022, MGI has more than 2,800 employees, and...
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01 Product Introduction Technical Principle Hardware platform Performance parameters Adapted applications 02 Packages Versatile library preparation, sequencing & analysis package 03 Applications Pathogen detection WGS Single Cell sequencing Oncology 04 Appendix Hardware parameters Ordering Information MGI Global Presence Flexible, high quality Activate your daily sequencing capability Leading Life Science Innovation
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DNBSEQ-G400* is a versatile benchtop sequencer providing users with comprehensive, flexible and efficient sequencing options.The high-throughput sequencing reagents (StandardMPS), provide more choices for users in pursuing higher sequencing quality. With stable high-intensity signals and random low sequencing error rate, exhibit excellent performance in scientific and clinical applications. DNBSEQ-G400* sequencer supports a wide range of applications including scientific research, clinical research, disease prevention, environment studies and agriculture, etc., increasing the popularity of...
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Technical principle MGI’S PROPRIETARY INCREASED ACCURACY DECREASED DUPLICATES Patterned Array DNA Nanoballs (DNBs) REDUCED INDEX HOPPING DNA Nanoball sequencing technology - No accumulation of amplification errors Hardware Platform Powered by 4-color and DNBSEQTM technology, DNBSEQ-G400* sequencer utilizes an innovative flow cell system which can support various sequencing modes and an optimized optical and biochemical system that enables the whole sequencing process to be completed within a short period of time, offering the user a simplified and streamlined sequencing experience. Control...
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Genetic Sequencer DNBSEQ-G400RS* 2006A fully automated workflow & all scenarios applicable Library preparation (Manual/ Automated) MGI ZLIMS Lite i PCR Product pooling Quantification & DNB loading (~15 min manually) Quantification (~10 min manually) Report either manual or automated * The maximum number of effective reads and data output are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method. ** Run time was calculated based on dual flow cell mode, and includes sample loading, sequencing, base calling and...
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Recommended sample numbers for a single run on DNBSEQ-G400* Sample numbers are calculated considering pooling variation and applications. For reference only. Leading Life Science Innovation 04
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Application Cases Pathogen detection Case 1: Pathogen detection - COVID-19 Sample: 6 serial dilutions of extracted RNA from isolated culture were subjected to ATOPlex Sequencing and RT-qPCR. Sequencing Platform: DNBSEQ-G400* Results: Table 1 Summary of ATOPlex Sequencing #SARS-CoV-2 detection; SARS-CoV-2% < 0.05%, negative; SARS-CoV-2% > 0.1%, positive; SARS-CoV-2% = 0.05-0.1%, gray zone. Conclusion: ATOPlex Sequencing can detect SARS-CoV-2 with 10 gradient dilutions (about 10~100 copies per ml) and assemble nearly full-length genome with 10 gradient dilutions (about 100~1000 copies per ml).
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WGS Case 2: Human WGS Sample: 1025 DNA samples of Han Chinese in the Central Plains Library: MGIEasy PCR-Free DNA Library Preparation Set Sequencing Strategy: DNBSEQ-G400* FCL PE150 Results: Table 2-1 Sequencing data quality 1) The raw depth of more than 98% of the samples is above 30X, with a lowest raw depth of 29.226X; 2) The Q30 ratios of the bases in all samples are over 85%; 3) Except for one sample (97%), the mapping rates of all other samples are greater than 99.5%; 4) More than 95% of the samples have a duplication rate of less than 2%, with the highest rate also less than 4%; The...
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Single-cell sequencing Case3: DNBelab C4 RNA sequencing DNBelab C Series Single-cell Omics Package is comprised of DNBelab C4 Pocket Single-Cell Lab, DNBelab C Series Single-cell Library Preparation Set, DNBSEQ™ sequencing platform and Single-cell Analysis Suite, all as part of a portable, instant, and one-stop single-cell research workflow. Sample: Human 293T cell line: Murine 3T3 cell line =1:1 Library Preparation: DNBelab C Series Single-Cell Library Preparation Set Sequencing Strategy: DNBSEQ-G400* FCL PE100 Data Results: Table 3-1 Cell data results Read Counts UMI Counts Gene Counts...
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Leading Life Science Innovation 08
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Leading Life Science Innovation 10
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MGI Genetic Sequencers DNBSEQ-E25* Reads per flow cell: 25 M Number of flow cells: 1 Data output: 2.5-7.5 Gb Reads per flow cell: 100-500 M Number of flow cells: 1 Data output: 10-150 Gb Reads per flow cell: 300-1800 M Number of flow cells: 2 Data output: 55-1440 Gb Reads per flow cell: 5800 M Number of flow cells: 4 Data output: 1-7 Tb Reads per flow cell: 80 M Number of flow cells: 2 Data output: 8-96 Gb Reads: 40 B Number of slides: 6 Data output: 48-72 Tb
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Technical Support Available Globally Local technical support and Customer Experience Centers (CECs) have been established in multiple countries and regions worldwide to ensure timely and effective technical support and training. Local warehouses and spare part centers have been established in multiple countries and regions worldwide to ensure the continuous availability of machine parts for maintenance. Online technical support is available globally with a fully functional call center (Toll-Free Hotline 4000-688-114) accessible during workdays from 9:00 AM-12:00 PM and 13:00 PM-18:00 PM...
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