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- ExoCAS-2
ExoCAS-2
ExoCAS-2
The document describes a magnetic bead-based ion exchange platform designed for the isolation of exosomes. Exosomes, due to their negative charge, can easily bind to magnetic beads coated with polycationic polymers. The process allows for rapid elution and isolation of exosomes.
- High purity and high recovery exosome isolation kit.
- Extracts three times more extracellular vesicles (EVs) than similar products.
- Short operating time of 20 minutes.
- No ultracentrifugation required.
- Purifies EVs from a wide range of biofluids, including plasma, cell culture media, and urine.
- ExoCAS-2 Mini/Maxi series for plasma, serum, and urine samples.
- ExoCAS-2 CCM series specifically designed for cell culture media.
The ExoCAS-2 exosome isolation kit is presented as a superior alternative to conventional methods such as ultracentrifugation and other commercial kits. It offers high yield, high purity, and high repeatability when isolating exosomes from various biofluids. The kit supports higher exosomal RNA concentration and shows the highest levels of exosome-specific markers (CD9, CD81, TSG101, ALIX) with the lowest level of total carryover protein (Albumin).
The protocol involves exosome clustering and scattering using the ExoCAS-2 kit. The kit provides the highest recovery yield and purity ratio of EV isolation, with a performance chart indicating superior results compared to other methods.
Validation through Western Blot Assay shows the highest RNA concentration and specific protein markers for exosomes, confirming the kit's effectiveness.
- ExoCAS-2 Mini Kit (20 preps) and Maxi Kit (50 preps) for plasma, serum, and urine.
- ExoCAS-2 CCM050 Kit and CCM250 Kit for cell culture media.
- Includes magnetic bead solution and elution buffer.
Catalog excerpts
AAAAA/\/\/\AAAAA xxxxxxxxxxxxx XXX xxxxxxxxxxxx XX xxxxxx: xxxxxx) XX X EXOSOME ISOLATION methods such as repeatability when XX xxxxxxxxxxxx xxxxxxxxxxxxx XXXXXX) XXXXXX) X XXXXXXXXXX) XXXXXX)E xxxxxx>E xxxxx: xxxxxxx X XThe Microgentas ExoCAS-2 exosome isolation kit is a superior alternative to conventional ultracentrifugation and other commercial kits. ExoCAS-2 can offer high yield, high purity, and high isolating exosomes from a wide range of biofluids. PLASMA / SERUM / CELL CULTURE MEDIA / URINE Exosome Clustering and Scattering : ExoCAS-2 Method •ExoCAS-2 Mini/Maxi series for plasma, serum and urine samples •ExoCAS-2 CCM series specifically designed for cell culture media Protocol Magnetic bead-based ion exchange platform designed to isolate exosomes. Exosomes can readily bind to magnetic beads coated with polycationic polymers due to their negative charge. Exosomes are rapidly processed through the elution step and can be isolated. Features • Rapid and convinient high purity and high recovery exosome isolation kit • Extracts 3 times more EVs than similar products • Short operating time : 20 mins • No ultracentrifugation required • Purifies EVs from wide range of biofluids, including plasma, cell culture media, and urine
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Purity Ratio(107 particles/ug protein) Highest Recovery Yield & Purity Ratio of EV Isolation
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- Molecular biology reagent
- Research reagent
- Laboratory reagent
- Diagnostic reagent
- Cytology reagent
- Buffer solution reagent
- Virus reagent
- Magnetic bead-based reagent
- Cell culture reagent
- RT-qPCR reagent
- Plasma reagent
- Western blot reagent
- Fungi reagent
- Protein analysis reagent
- DNA extractor
- Cell isolation reagent
- Clinical diagnostic DNA extractor
- Fully automated DNA extractor