ExoFilter

ExoFilter

ExoFilter

Product catalog summary
Introduction to ExoFilter™ Exosome Isolation Kit
Microgentas's ExoFilter™ is designed for large-scale extraction of extracellular vesicles (EVs), offering superior operational efficiency and recovery rates compared to traditional methods like tangential flow filtration (TFF). It is suitable for a variety of sample types and volumes, making it a versatile tool for exosome isolation.
Key Features
  • Large-Scale Sample Volume: Capable of processing from 1 mL to tens of liters, ideal for large-scale EV isolation.
  • Versatile Sample Compatibility: Compatible with a wide range of biofluids including plasma, serum, urine, saliva, and cell culture media.
  • Rapid and Simple Protocol: Offers quick processing (approximately 15 minutes) with high yield and purity, independent of operator skill.
Methodology
  • Charge-Based Isolation: Utilizes a positively charged filter to capture negatively charged EVs, effective even under dynamic flow conditions.
  • Protocol: Involves adding the sample to the filter, capturing EVs, and then eluting pure exosomes using an elution buffer followed by centrifugation or vacuum pressure.
Product Specifications
  • Available in various configurations (ExoFilter-001, ExoFilter-015, ExoFilter-050, ExoFilter-250) with different sample volume capacities ranging from 1 mL to 250 mL per test.
  • Each package contains 20 preparations.
Performance and Comparison
  • Yield and Purity: ExoFilter™ offers a high purity ratio and particle concentration, outperforming other commercial kits like EQ and EE.
  • Efficiency: Demonstrates an 81% EV recovery rate, comparable to EQ, with a significant reduction in protein concentration compared to ExoPAS™.
Conclusion
ExoFilter™ provides an efficient, versatile, and high-yield solution for exosome isolation, suitable for a wide range of applications and sample types.
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Catalog excerpts

ExoFilter-1

EXOSOME ISOLATION EXOFILTER™ Introducing Microgentas's ExoFilter™ exosome isolation kit - the optimal solution for large-scale extraction of extracellular vesicles(EVs). ExoFilter™ surpasses conventional methods such as tangential flow filtration(TFF) in terms of operational efficiency and recovery rate when handling substantial sample volumes. Elevate your exosome isolation process with the innovative ExoFilter™ kit. CULTURE MEDIA / PLASMA / SERUM / URINE / SALIVA etc. Processing Large Volume ( ~ 350 mL) High Yield & Purity Operator independent Charged based EV Isolation: ExoFilter™ ExoFilter™ Series The ExoFilter series accommodates large-capacity cell culture media (CCM) as well as smaller-volume samples, including plasma, serum, urine, and saliva. (EV-) Negatively charged EVs captured by (Filter+) positivity charged membrane filters - The maximum single-use capacity extends to 350 mL - The minimum suitable volume starts at just 1 mL *Customized products are available for substantial samples at liter levels. Method Charge-based adsorption filter for large-volume sample processing - A strong positively charged filter effectively captures negatively charged EVs, even under dynamic flow conditions. Microscopic images of ExoFilter structure - The capturing properties are nearly independent of flow speed, ensuring rapid separation of EVs for large-scale sample volume. x 1000 Features Large-Scale Sample Volume: provides the optimal solution for isolating extracellular vesicles (EVs) from large-scale sample volumes Versatile Sample Compatibility: Isolate EVs from a broad range of sample volumes, from 1 mL to tens of liters Wide Biofluid Compatibility: Enriches EVs from diverse biofluids, including plasma, cell culture media, saliva, and urine, making it a versatile tool for various applications Rapid operation with simple and easy protocol Protocol Sample Elution Buffer 1. Adding the sample to the filter, the filter captures extracellular vesicles(EVs). 2. By simply adding elution buffer(EB) to the filter, you can obtain pure exosomes through centrifugation or vacuum pressure.

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ExoFilter-2

Quick and easy, large volume EV isolation In comparison to ExoPAS™, ExoFilter™ demonstrated an 81% EV recovery rate which is nearly same as EQ. ExoFilter™ stands out with a remarkable 50% reduction of protein concentration compared to ExoPAS™. The protein of ExoFilter™ can be further reduced through an additional washing process. Performance Chart of Yield & Purity Ratio With high purity ratio and high particle concentration, ExoFilter™ significantly enhances EV isolation efficiency when compared to other commercialized kits, including EQ and EE. • Relatively high purity ratio • High yield Purity...

 Open the catalog to page 2

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.