EXOSOME ISOLATION EXOPAS™ The Microgentas’s ExoPAS™ exosome isolation kit is a superior alternative to conventional methods such as ultracentrifugation and other commercial kits. ExoPAS™ can offer high yield and high repeatability when isolating exosomes from a wide range of biofluids. Exosome Precipitation and Scattering : ExoPAS™ ExoPAS™ Series - ExoPAS Original series for plasma, serum, urine and saliva samples - ExoPAS CCM series specifically designed for cell culture media Method ExoPAS™ is a precipitation-based method, which increases EV isolation efficiency to the highest. The ExoPAS series kit that enables high recovery yield of exosome isolation. Using these unique, charge-based reagents, EVs are rapidly aggregated and precipitated from solution. EV Isolation and Clustering Isolated EV Rapid operating time: 15 min Simple and easy protocol Enrichment of EVs available from wide range of biofluids, including plasma, cell culture media, saliva, and urine Available Downstream applications: • miRNA profiling • NGS • mass spectrometry analysis.

EXOSOME ISOLATION ExoPAS™ particle concentration led to the best EV recovery rate among other EV isolation methods. RT-qPCR analysis was performed to identify EV-miRNAs. The results were consistent with the NTA analysis (EV-derived miRNAs ; has-let-7a-5p, has-miR-142-3p). To identify surface and internal protein markers of EVs, western blot assay was used. As a result, ExoPAS showed a thick band indicating a high number of EVs. ExoPAS™ increases EV isolation efficiency but often results in high impurity levels. By adding the washing step, purity has improved. "Washing Buffer (WB) is provided...