Micronbrane Medical
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Pathogen Identification Directly from Whole Blood within 24 Hours Pathogen Real-Time Identification by Sequencing (PaRTI-Seq™) is Micronbrane's complete solution for rapid Pathogen Identification. Combining Devin™ filter optimized sample , preparation method for next generation sequencing (NGS) and proprietary analytical methods, it provides precise test results within 24 hours. Sample Processing Library Prep & Sequencing Devin™️DNA Microbial Enrichment Kit Data Analyzing & Reporting Final Report PaRTI-Cular™ Analysis Ultralow DNA NGS Library Preparation Kit Devin™️Fractionation Syringe Filter Devin™ Microbial DNA Enrichment Kit Over 99% depletion of human automated workflows Zwitterionic Interface Compatible with different Ultra-Self-Assemble Coating (ZISC) sequencing platforms, qPCR, Unison™️Ultralow DNA NGS Library Prep Kit PaRTI-Cular™ Software Fast reporting: < 30 mins / sample Ultra Low Input starting from 10 pg of DNA Regularly updated pathogen database Hands-on time: 45 mins Proprietary reference database for comprehensive analysis Fast processing: 150 mins Advantages of PaRTI-Seq™ PaRTI-Seq™ Others mNGS Blood Culture Accurate Accelerated Affordable Automatable All-in-one Can identify all pathogens at the same time Blood Culture can detect pathogens only in 20-30% cases with long turnaround time (2-7 days). Blood culture test has multiple drawbacks for clinical application (some microorganisms are unculturable or hard to culture eg: Treponema pallidum, Bartonella spp. or take weeks to culture eg: mycobacteria and molds) Molecular tests like PCR can only detect predefined specific pathogens and require specialized kits and primers PaRTI-Seq™ allows to avoid host interference by patent technology and requires 4x less sequencing reads than other mNGS tests. It can detect even tiny amount of microorganisms (sensitivity 102 genome copies/ml) Core technology of PaRTI-Seq™ — filter Devin™ Devin™ is a disposable blood fractionation filter invented for wide use in clinical microbiology. It enables faster more accurate and cost effective way to detect pathogens in human blood for further , analysis such as genetic tests (DNA sequencing, PCR etc.), virology, immunology and other applications. ZISC Technology >99% pathogens output Before Microbial enrichment fluids, such as whole blood, plasma, CSF, BALF etc. It utilizes a patent technology of Zwitterionic Interface Ultra-Self-assemble Coating (ZISC) which allows to deplete human nucleated cells from the sample (Fig. 1) while allowing most of microorganisms (including bacteria, viruses, fungi etc.) to go through the filter intact (Fig. 2). Devin™ effectively removes host DNA interference from body In comparison with other depletion methods ( ig. (less than 3) Devin™ shows much faster processing time 5 minutes) and greater ratio of microbial sequencing reads. Fig. 1 Leukocytes reduction efficiency Effect of Devin on virus passing efficiency Effect of Devin on microbial Test concentration Fig. 2 Microbial passing efficiency Without Devin™ With Devin™ How Devin™️helps to save up to 75% of sequencing cost? Fig. 3 Comparison of different host depletion methods sequencing output requirment. Human I. Halo A. Halo Relative cost Host DNA interference presents the biggest portion of sequencing cost. Devin™ filter allows to remove most of host DNA interference in the sample and reduce up to 75% of sequencing cost due to lower *Results of human and microbial identification using qPCR, NGS and Nanopore. Human blood samples (5 mL) were added. 10*4 Genome Copies/mL spike in control from ZYMO Research and Nucleic acid isolation Library preparation then processed using different depletion methods. Test results show that Devin™ filter increases ratio of microbial DNA and decreases the ratio of human (host) DNA. No. 99, Xinpu 6th St., Taoyuan Dist., Taoyuan City 33044, Taiwan Become our partner today: test workflow