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tNGS Product

Product catalog summary

Product Introduction
PTseqTM is a high-throughput genetic sequencing product designed for pathogen targeting. It focuses on 232 pathogenic microorganisms, 8 classes of 31 drug resistance genes, and 6 classes of 11 virulence genes, specifically for respiratory syndrome. The product combines multiplex PCR and NGS technology to enrich and sequence targeted genes using the MGI sequencer. This approach efficiently identifies suspected pathogenic microorganisms, drug resistance genes, and virulence gene information, improving pathogen diagnosis rates and aiding in the development of individualized anti-infection treatment strategies.

Applicable Population
The product is suitable for hospitalized patients requiring primary screening for infectious diseases, addressing over 95% of related issues. It is also applicable to critically ill patients and those needing drug resistance gene identification, such as suspected TB drug-resistant patients.

Detection Range
The product supports various respiratory samples, including BALF, sputum, swabs, and other respiratory fluids. It covers 274 targets, including bacteria, viruses, fungi, and other pathogens, with a data volume of ≥300K Reads and a turnaround time (TAT) of up to 10 hours. The limit of detection (LOD) is up to 50 copies/mL.

Classification and Detailed List of Targets
The product identifies 72 bacterial species, 95 viral species, 50 fungal species, and 15 other pathogens. It also detects 31 resistance genes and 11 virulence genes. Examples include Streptococcus pneumoniae, Mycobacterium tuberculosis, influenza viruses, Candida albicans, and various resistance and virulence genes.

Platform and Advantages
The DNBelab-D4/MGISP-100 system offers automated sample preparation with high specificity and sensitivity. The DNBSEQ-G99/E25 is a high-throughput sequencer that is lightweight, modular, and automated, providing a comprehensive localized tech-transfer service. The system is cost-effective, does not require microbial culture, and offers fast pathogen detection with no background interference. It covers over 95% of respiratory tract infection pathogens and is more clinically accessible compared to mNGS.

Additional Features
The product includes the Halos all-in-one bioinformatic analyzer, offering wide coverage and simultaneous DNA and RNA analysis.

Catalog excerpts

tNGS product

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Product Introduction PTseqTMpathogen targeting high-throughput genetic sequencing ✓ 232pathogenicmicroorganisms+8classesof31drugresistancegenes+ 6classesof 11virulencegeneswere selectedforrespiratorysyndrome ✓ CombinedwithmultiplexPCRandNGStechnology,targetedgeneswereenrichedandsequencedusingMGI sequencer It can efficiently identify suspected pathogenic ✓ microorganisms such as fastidiosa, intracellular bacteria, fungi, DNA and RNA viruses, as well as drug resistance genes and virulence gene information, significantly improve the positive rate of pathogen diagnosis, assist clinicians to develop...

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Classification and detailed list of 274 targets Type Bacteria (72) Target name Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus constellations, Staphylococcus aureus, Mycobacterium tuberculosis complex group, Mycobacterium avium complex group, Bacillus anthracis, etc. Yersinia pestis, Pseudomonas aeruginosa, Legionella pneumophila, Acinetobacter baumannii, Escherichia coli, Streptobacillus moniliformis, Bordetella pertussis, etc. Monkeypox virus, human boca virus (type 1, type 2, 3, 4), adenovirus(human adenovirus mammal group A and group B, group C, D, E group,etc. Influenza A...

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Automated sample preparation system High-throughput sequencer All-in-one bioinformatic analyzer Lightweight + Modularity + Automation +Comprehensive localized tech-transfer service High specificity High sensitivity Wide coverage Identification is based on species-specific sequences No background interference + positive enrichment Covers more than 95% of respiratory tract infection pathogens Highcost effective Direct extraction of nucleic acid, Microbial culture is not required Simultaneous DNA and RNA pathogen detection for fast results Significantly lower cost and more clinically accessible...

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