SpectraMax Glo Steady-Luc Reporter Assay Kit Luciferase measurement for your microplate reader assay KEY FEATURES • Glow-type luminescence provides an extended signal time window enabling batch processing of plates in screening assays • Optimized for luminescence microplate readers • Simplified data acquisition and analysis with preconfigured protocols in SoftMax Pro Software Reporter gene assays are an important tool for biomedical and pharmaceutical researchers to monitor cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP. The application of luciferase-based reporter assay using luminescence microplate readers has become increasingly popular for high-throughput analysis of gene expression as well as drug discovery applications. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Using a homogeneous protocol, the specially formulated reagent mixture in this kit modifies the activity of luciferase for a significantly extended signal window with half-life around three hours, therefore enabling batch processing of plates in screening assays. This assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.

Easy workflow The SpectraMax Glo Steady-Luc Reporter Assay Kit offers a homogeneous workflow which is optimized specifically for microplate readers. A typical experimental workflow is presented in Figure 1. Add reagent into cell Luminescence measurement Data analysis log[L uciferfas e] fg/well SpectraMax i3/i3x Multi-Mode Microplate Reader Figure 1. Representative experimental workflow for reporter assay. Optimal data quality for microplate reader assay The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both...

Ordering information The SpectraMax Glo Steady-Luc Reporter Assay Kit is available in two formats for different throughput requirements. The Explorer kit is sufficient for two 96-well microplates whereas the Bulk kit is sufficient for ten 96-well microplates. The number of microplates is based on the example protocol validated by Molecular Devices. *Currently only available for US customers Compatible with Molecular Devices microplate readers • SpectraMax® i3/i3x Multi-Mode Microplate Reader • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate...