SpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold improvement in throughput • Autorinse injectors for robust performance • 9 orders of dynamic range • IQ/OQ, software, and FDA CFR 21 Part 11 compliance tools • Industry-leading SoftMax Pro Software The SpectraMax® L Microplate Luminometer from Molecular Devices® offers the sensitivity, reliability, flexibility, upgradability, automation options, and validation tools required by today’s leading laboratories. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. The SpectraMax L Reader is upgradeable to multi-channel configurations for a 6-fold increase in throughput for medium throughput and secondary screening laboratories. Industryleading SoftMax® Pro Microplate Data Acquisition and Analysis Software eliminates the need to export data to spreadsheet programs, and manages instrument control and results generation in a GxP environment. SoftMax Pro GxP Software and its software validation package provide comprehensive documentation and extensive data sets in CD-ROM format to meet FDA 21 CFR Part 11 and GxP compliance requirements. Reduced noise Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and the lowest crosstalk in laboratory assays. Nine orders of dynamic range The simultaneous analog and photon counting capability provides a dynamic range of more than nine orders of magnitude, eliminating the need to dilute bright samples while maintaining excellent sensitivity for dim samples. Up to 6-times faster The SpectraMax L Reader is available in 1-, 2-, and 6-channel (Photomultiplier Tube) configurations. The 6-channel configuration is available with 0 or 12 injectors for a 6-fold increase in throughput for flash and glow assays including GPCR via aequorin (Figure 1) and dual luciferase reporter gene screening. The 2-channel configuration is available with 0, 2, or 4 injectors. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for tradi

Figure 1. Multi-channel GPCR agonist screening Figure 4. Integrated stacker option Figure 3. User-friendly software Figure 2. Multi-channel precision PMT F 
 AqueoZen H1 cells expressing histamine H receptor. EC curves are shown for histamine (blue) and HTMT (red). Read on a 6-channel SpectraMax L Reader. 1 PMT to PMT RLU precision on four wells ranging in brightness from 8,000 to 1,600,000 RLU read on a 6-channel SpectraMax L Reader using the automatic internal LED PMT calibration method. glow assays. The 2-injector configuration enables flash and glow BRET assays, a 2-fold increase in speed...