External Perfusion

Product catalog summary

External Perfusion System Overview
The External Perfusion System for the Port-a-Patch® is designed for reliable and fast perfusion, featuring computer control and synchronization between perfusion and data acquisition. It includes an integrated vacuum for waste removal and can be used as a stand-alone system.

System Components and Functionality
The system comprises a computer-controlled magnetic pinch valve panel and a dedicated flow chamber. It can handle up to eight different solutions, with manual or automatic control for solution switching. Automatic control allows synchronization with data acquisition and tagging of traces with compound and concentration information.

Flow Chambers
Two flow chambers are available: the standard Laminar Flow Chamber and an additional chamber for ultrafast perfusion, allowing solution switch times down to 15 ms. This makes the system ideal for studies of ligand and voltage-gated channels. Temperature control is also available.

Performance and Applications
The system supports reliable compound application, with ligand-dependent activation of GABAA receptors and pharmacological experiments on hGlyRα1. The Laminar Flow Chamber allows a solution switch time of approximately 100 ms, while the Fast Perfusion Chamber achieves exchange times down to 12 ms.

Product Specifications
The External Perfusion System is available in different configurations, with or without the perfusion chamber. The Valve Control Panel measures 40 x 5.8 x 13 cm and weighs 2.45 kg. Kits include necessary accessories such as syringes, tubing, and reagent kits.

Key Data and Observations
Graphs and data illustrate the system's capability in handling different concentrations and compounds, with specific examples of GABA and glycine receptor studies. The EC50 for glycine was determined as 89 ± 2.7 µM, consistent with literature values.

Catalog excerpts

Port-a-Patch® - reliable and fast perfusion. • Fast and continuous perfusion • Computer control • Synchronization between perfusion and data acquisition • Integrated vacuum for waste removal • Also available as stand-alone system

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is an additional perfusion chamber comprised of a computer controlled magnetic pinch allowing solution switch times down valve panel, and a dedicated flow chamber for the Port‑a‑Patch®. Up to eight different solutions can be configuration. The External Perfusion handled by the magnetic valve panel. Switching of System is thereby ideal for studies of both ligand and voltage gated automatically. The latter allows synchronization of data channels. Temperature control is acquisition with solution exchange, as well as tagging also available in combination with the collected traces with relevant...

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External solution exchange. Currents of RBL cells endogenously expressing K+ permeable channels (-100 mV) were measured. The external K+ concentration was changed using the Perfusion System and the standard, Laminar Flow Chamber. Time constant for solution exchange was approximately 100 ms. Low K+ (4.5 mM K+), high K+ (143 mM K+). PHOTO LAMINAR FLOW CHAMBER PHOTO LAMINAR FLOW CHAMBER Exchange time: 100 ms Exchange time: 100 ms Reliable compound application. Ligand dependent activation of GABAA r eceptors can be recorded with the aminar L Flow Chamber, allowing a solution switch time of approximately...

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Ultrafast perfusion of GABA. Using the Fast Perfusion Chamber GABAa (al P2y2) channels were challenged with 100 jM GABA, eliciting a fast on-set of the whole cell currents (holding potential -80 mV). Peak current amplitudes were reached within 20 ms.

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External Perfusion

External Perfusion

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