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Nanion Technologies
The Port-a-Patch

The Port-a-Patch

Product catalog summary

Overview: The Port-a-Patch NPC-1 is a compact patch clamp system designed for rapid and high-quality data generation in ion channel research. It is user-friendly, suitable for both experts and novices, and supports various recording configurations including whole-cell, cell-attached, perforated patch, and bilayer recordings.

Key Features:

Utilizes a borosilicate glass chip with a micron-sized aperture for cell capture and sealing.
Compatible with most amplifiers, including the EPC10 amplifier.
Supports voltage and current clamp, whole-cell, and single-channel recordings.
Includes versatile add-ons such as external and internal perfusion systems, temperature control, and optical stimulation capabilities.
High success rates with primary cells, stem cells, and various ion channels.

Technical Specifications:

Chip resistance: 2 - 3.5 MΩ, seal resistance: > 1 GΩ, whole cell resistance: > 1 GΩ.
Liquid consumption: ~30 μl/compound, perfusion time constant: ~100 ms.
Whole cell stability: ~20 min, throughput: 20-50 data points/day.
System includes a recording station, suction control, PatchControl software, and EPC-10 USB amplifier.

Applications and Success:

Validated with numerous ion channels in various cell types, including primary cells and stem cells.
Successfully tested with voltage-gated, ligand-gated, and other ion channels.
Endorsed by researchers for its ease of use and ability to handle challenging cell types.

Testimonials:

Professor Armin Kargol highlights its suitability for undergraduate research, reducing training time significantly.
Prof. Carlos Villalobos praises its performance in recording small calcium-release activated currents in difficult cell types.

Contact Information: Nanion provides contact details for their offices in Europe, USA, China, and Japan, along with distribution partners in several countries.

Catalog excerpts

The world's smallest patch clamp setup. Providing biggest results.

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The Port-a-Patch. Enjoy electrophysiology • Increased throughput with high data quality • Whole-cell and single channel recordings • Easy-to-use – also for non-experts • Successful with primary cells and stem cells • High success rates for stable whole cells • Voltage and ligand gated ion channels • ersatile liquid handling - internal and V • timulating Optical Lid (SOL) developed for S external perfusion systems available optical stimulation of ion channels • ompatible with most amplifiers C • Temperature control possible (cooling and • oltage and current clamp V The Port-a-Patch is a miniaturized...

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chip resistance: 2 - 3.5 MΩ (customized resistances available) seal resistance: whole cell resistance: series resistance: liquid consumption: perfusion time constant (Perf. Sys.) internal perfusion time constant: avg. whole cell stability: successful whole cell recording: 70 - 90 % (consistent between cell lines) Technical Specifications A Port-a-Patch system includes: • ort-a-Patch recording station P (including Faraday top) • ort-a-Patch Suction Control, USB-controlled P (no house vacuum needed) • atchControl software (Windows) including P sophisticated graphical tools for logging of events...

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Smart tools for ion channel research “Nanion’s Port-a-Patch is an excellent instrument for research labs working with undergraduate students. Manual patch-clamping methods depend to a large extend on the skills of the operator. They require long training periods. For a typical undergraduate student it takes over a year to learn patch-clamping. Port-a-Patch reduces that time to only weeks and the most difficult part is to understand the software that controls the instrument. This allows undergraduate students to actively participate in real research projects.” Professor Armin Kargol, Loyola University...

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The Port-a-Patch

The Port-a-Patch

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