SyncroPatch 384/768PE. Patch clamp meets HTS.

SyncroPatch 384/768PE. Patch clamp meets HTS. (modular approach) (completed recordings) • Giga-seal recordings • Reliable data from diverse • 20,000 data points per day • Cost-efficient high throughput screening State-dependent inhibition by increasing tetracaine concentrations (5 µM, 50 µM and 500 µM). The CHO-cells were kindly provided by Anaxon. Reliable and efficient liquid handling 2. Analyzed data Whole-cell response of hα1GlyRs to increasing concentrations of g ­ lycine (left), and repeated exposures of hGABAA α1,β2,γ2 receptors (right) to GABA. Multi-hole chips were used. recording substrates...

SyncroPatch 384/768PE Technical Specifications: average whole cell-stability successful whole-cell recordings throughput per PE-module seal resistance series resistance chip resistance ~3 MΩ (different ranges available) perfusion time constant <50 ms minimum exposure time compatible liquid handling robots amplifier channels HTS screening of stem cell-derived cardiomyocytes Benefit from our experience The SyncroPatch 384/768PE is based on proven, Approximately 300 stem cell-derived cardiomyocytes are required per recording well (Cor.4U, Axiogenesis). Just 1.5 million stem cells are sufficient...

DataControl 384 software suite. Efficient data handling and export. PatchControl 384 PatchControl 384 is a powerful graphical user ­nterface for i intuitive, quick and easy setup of voltage protocols and experimental parameters. As can be seen from the overview on the right, the recording wells are v ­ isualized and color-coded based on ­ ser-defined u quality criteria, e.g. seal resistance, series resistance, and capacitance. With one mouse click, the view switches to online analysis results, for ­ ­ example ­I/­V­curves or concentration-response curves. DataControl 384 loads and analyzes the...

PatchControl 384 – an overview 384 recording wells color-coded based on set quality criteria Experiment statistics Single well highlighted Close up selected traces In this data set, one wash addition and three concentrations of quinidine were a ­ dded to CHO cells expressing hKv1.3. Increasing concentrations of compound are represented by the blue segments. The cells were kindly provided by Evotec. Hardware and software work hand in hand A complete run on the SyncroPatch 384/768PE takes 1 ­ 5-20 ­ inutes for four additions, e.g. one control and m three concentrations. Data is analyzed as the...

Smart tools for ion channel research ‘The SyncroPatch 384PE is a landmark in the history of automated patch clamping. It follows a completely revolutionary concept: a truly modular ­ and HTS-capable automated patch clamp device that will integrate into your existing or planned screening process line, rather than being a closed, stand-alone system. No other platform on the market is ­capable of recording 384 to 768 cells in parallel, let alone with ­giga-seals. In development, we have carefully listened to key customers from ­ industrial pharma high throughput screening laboratories to fulfil...