Temperature control

Product catalog summary

Temperature Control Overview
The Port-a-Patch® system offers precise temperature control, enabling experiments at physiological temperatures and brief temperature pulses. It supports continuous perfusion, making it suitable for studies involving hERG and TRP channels.

System Components and Functionality
The system includes a canulla for heating solutions during perfusion and a flow chamber with a resistive heating element. It allows temperature adjustments from room temperature to 60 °C with a precision of ± 0.5 °C, typically reaching the desired temperature within two minutes. Cooling is passive, and the system is controlled via a computer interface.

Applications and Benefits
The Temperature Control System is ideal for studying ion channels with temperature-dependent properties, such as hERG and heat-activated TRP channels. It enables recordings at physiological temperatures, enhancing the accuracy of pharmacological experiments. For instance, quinidine's effect on hERG channels was consistent at both room and physiological temperatures.

Experimental Data
Example traces show hERG-mediated currents at 25 ± 2 °C and 35 ± 2 °C, with increased peak current amplitude and faster kinetics at the higher temperature. TRPV1 current responses to voltage ramps demonstrate the system's capability to handle temperature variations effectively.

Product Specifications
The Temperature Control System is available with or without a perfusion system. The complete system includes a valve control panel, laminar perfusion chamber, resistive heating element, and temperature monitoring system. The system's dimensions and weight are specified for both configurations.

Additional Components
An additional perfusion canulla with a heater and sensor is available for enhanced functionality.

Catalog excerpts

Accurate maintenance of temperature • Brief temperature pulses possible • Experiments at physiological temperatures • Allows continuous perfusion

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The Temperature Control System for the Port-a-Patch® allows experiments at elevated temperatures or application of short temperature pulses. Two components are used to heat the solutions: a canulla for heating of solutions during continuous perfusion, and the flow chamber containing a resistive heating element. This results in stable temperature maintenance whether or not the solutions are perfused. A computer interface is used to monitor and control the applied temperatures, allowing temperature changes with the click of a button. Temperatures ranging from room temperature to 60 °C (± 0.5 °C) can...

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Temperature control up to 60 °C. Nanion’s e nables recordings from room temperature up to 60 °C. In this way, ion channels with temperature k inetics or opening probabilities can be s tudied, for example hERG channels and heat activated TRP channels. Heat activated channels. Original traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV. TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C and 36°C), and after cooling down to RT (29°C). The right-hand picture shows the current amplitudes as recorded at -100 mV and +100 mV plotted...

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