Vesicle Prep Pro

Product catalog summary

Overview
The Vesicle Prep Pro is an automated system designed to simplify the preparation of giant unilamellar vesicles (GUVs), which are crucial for studying biophysical properties of lipid membranes and transmembrane proteins. The system ensures high reproducibility and efficiency in generating solvent-free GUVs through electro-swelling.

Technical Specifications

Success rate of GUV formation: 100%
Vesicle size range: 1 – 30 µm, with an average size of 10 µm for 60-70% of vesicles
Preparation time: 20 – 300 minutes, depending on lipid and protocol
Voltage amplitude: +/- 10 V (max. 20 V peak to peak)
Amplitude resolution: > 10 mV
Frequency: 0 – 1000 Hz
Output current: max. 100 mA
Liquid capacity: 200 μl – 900 µl
Temperature range: Room Temperature (RT) – 70°C
Maximum salt concentration: 50 mM (NaCl or KCl)

Components

Vesicle Prep Pro station: 16 x 14 x 6.5 cm, 1.8 kg
Vesicle Prep Chamber: 8.3 x 6 x 1.3 cm
Integrated temperature control and ITO slides
VesicleControl software (Windows)

Features

Automated and standardized GUV preparation
Optical monitoring of vesicle formation
Flexible protocol design
Temperature control for high charge or phase transition lipids
Stable bilayers for electrophysiological recordings

Applications and Data
The system has been successfully tested with various lipids and proteins, including ion channels and porins. It supports high-resolution, low-noise recordings of single-channel activity, making it ideal for ion channel research.

User Testimonials

Colin Taylor, University of Cambridge, highlights the system's ease of use and efficiency in studying recombinant channel proteins.
Eric Stava, University of Wisconsin-Madison, praises the system's utility in bioelectronics research and its ability to visualize the electro-swelling process.
Marisa Rangel, University of Sao Paulo, notes the system's effectiveness in characterizing pore-forming peptides in artificial bilayers.

Contact Information
Nanion provides contact details for their offices in Europe, USA, China, and Japan, along with distribution partners in several countries.

Catalog excerpts

Liposomes made easy. Vesicle Prep Pro nan]i[on

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Success rate GUV formation: Vesicle size: Average size: Preparation time: 20 – 300 minutes (lipid/protocol dependent) Voltage amplitude: +/- 10 V (max. 20 V peak to peak)Success rates Amplitude resolution: Output current: Temperature range: Maximum salt concentration: Technical Specifications The Vesicle Prep Pro System includes: • Vesicle Prep Pro • Vesicle Prep Chamber • Integrated temperature control • ITO slides • esicleControl software (Windows) V Size and weight: • esicle Prep Pro station: V S ize (l x w x h): 16 x 14 x 6.5 cm Weight: 1.8 kg ITO Slides Solution Lipid Layer • esicle Prep...

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Vesicle Prep Pro Liposomes made easy • Easy and quick preparation of GUVs • Temperature control • Solvent free membranes • High reproducibility • Optical monitoring of GUV formation • Stable bilayers for ephys recordings Giant unilamellar vesicles (GUVs) are an essential oxide (ITO) turning them into electrodes. This leaves the component of techniques used in the investigation of chamber transparent so that vesicle formation and growth can be monitored optically throughout the entire membranes, raft process. Integrated features, including flexible protocol formation, and lipid-DNA interactions....

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Smart tools for ion channel research “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.” Colin Taylor, Professor of Cellular Pharmacology, Department of Pharmacology, University of Cambridge, Cambridge, UK “The Vesicle Prep Pro has been of great utility in our Bioelectronics...

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Vesicle Prep Pro

Vesicle Prep Pro

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