HAPPY CELLS. BETTER SCIENCE.
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HAPPY CELLS. BETTER SCIENCE. Sterile, gentle microfluidics to analyze, sort and plate.

Cell sorting made for every lab. Whether you are focused on biopharma, genomics, or basic research, NanoCellect® delivers sterile, portable, intuitive solutions for gently sorting samples. The WOLF® Cell Sorter and N1 Single Cell Dispenser are designed to empower biomedical scientists and accelerate research. With WOLF’s compact size and price, you can bring the convenience of FACS cell sorting to YOUR benchtop. iPSCs CRISPR Sterile Plate Seeding (fits in hood) Sterile, Gentle Sorting Plate Viable Single Cells Single-Cell Plating Image Single Cells Scale-Up

Single-cell Genomics Sort individual cells into plates or tubes for higher quality sample preparation. The WOLF can improve your cell purity, viability and RNA integrity, allowing you to select and enrich the cells of interest. There’s no longer a need to sequence and analyze debris, dying or off-target cells. Cell Line Development Giving scientists the power to find the best cell. Say goodbye to the inefficiency of limiting dilution, and the low viability of conventional cell sorters. When paired with the N1, the WOLF can select viable single cells and plate them into 96- or 384-well...

WOLF Cell Sorter The WOLF Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup. Healthy Cells Simple & at YOUR Bench At <2 psi, the WOLF is gentler than any conventional cell sorter, enabling healthier cells post sort, especially for engineered lines, primary...

Bulk Sorting Beads: A mixture of 5μm and 15μm Dragon Green beads (FITClike) was prepared and sorted on the WOLF Cell Sorter. Transfected Cell Lines: To assess the sorting performance of our instrument, mixed population of MCF-7 GFP-positive and GFP-negative cells were prepared and sorted. GFP-positive cells were sorted to a 98% purity. N1 Single Cell Dispenser Designed to sort and dispense into 96and 384-well plates, the N1 provides higher rates of singlet detection compared to cell printers or limiting dilution. Users can perform simple, labelfree dispensing or advanced multicolor panel...

High Viability • The WOLF’s gentle sorting technology results in improved viability of cells and higher outgrowth • Reduced stress during a sort also avoids potential gene expression or karyotype changes experienced with traditional sorters Sterile Microfluidics • Disposable microfluidic cartridge • 100% disposable fluidic pathway • No biohazardous aerosol exposure • No sample-to-sample contamination Fluidic Flexibility • The WOLF uses a small fraction of the sheath used by traditional sorters, reducing costs and allowing users to use growth media as sheath fluid • Using growth media as...

Single Cell Efficiency The 5 parameters of the WOLF and N1 provide higher rates of singlet detection, and live/dead discrimination, compared to cell printers or limiting dilution.

The WOLF Cell Sorter and N1 Single Cell Dispenser dispensed beads with a singlet fidelity of 92.26% on 96-well plates and 93.27% on 384-well plates. Similarly, dispensing CHO cells resulted in 90.84% and 90.6% single cells into 96- or 384-well plates, respectively (Figure 1). These results are comparable to traditional FACS technology, yet with significantly lower mechanical stress. High shear stress commonly reduces colony outgrowth to below 30% with FACS. However, with the WOLF low-pressure microfluidics, 86% and 87% of wells had monoclonal outgrowth after 14 days in both 96-and 384well...

Microfluidic Cartridges The WOLF Cell Sorter has several application-specific cartridges: analysis-only, bulk/tube sorting and plate sorting. Individual cartridge and tubing sets are sterile-packed to avoid cross-contamination. A key design feature is that everything that the sample and sheath fluid touches is sterile and disposable. A gentle sorting mechanism results in improved viability of cells and higher outgrowth, compared to conventional sorters. • No aerosols are produced during sorting • Uses only a small fraction of sheath (~50 mL for an entire day) • Cleanup in 1 minute: once...

WOLF Cell Sorter Specifications Optics Excitation 488 nm; 30 mW diode laser (rated at 45,000 hr life) Laser Profile Scatter Detection Forward (0 Degrees, +/-15) Back (180 Degrees, +/-15) Emission Detection Optical Alignment 3 Colors, user exchangeable bandpass filters Standard set installed: • 525 ± 25 nm (FITC, GFP) • 585 ± 20 nm (PE) • 665 Longpass (PI, PE-CY5, mCherry, tdTomato) Fixed alignment, no maintenance required Fluidics Sample Input Sheath Input Sheath Fluid Sheath Fluid Usage Sample Flow Rate Sheath Flow Rate Dead Volume Minimum Sample Volume Tubing Diameter (Inner) Flow Cell...

Performance Scatter Resolution Resolves lymphocytes, monocytes & granulocytes Fluorescence Resolution 8 peak separation with the Spherotech Rainbow calibration beads in the 3 fluorescence channels Analysis Speed Back-to-back Sorting Speed Absolute Counts Volumetric Analysis Warm-up Time Less than 1 minute Sorting Output Sorting Purity

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