Nanodigmbio-μCaler Total Solution for Methylation
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^Caler Total Solution for MethylationBackground According to data from IARC, 19.29 million new cancer cases globally occurred in 2021, with about 9.96 million deaths. The global cancer burden is expected to reach 28.4 million cases in 2040, reflecting a 47% increase from 2020. Additionally, the five-year survival rates of cancer patients vary among different countries. The reasons for this are multifaceted. On one hand, the majority of cancers are commonly detected at a later stage, with most patients diagnosed at an intermediate or advanced stages, resulting in poor intervention outcomes. On the other hand, traditional early screening methods for cancer have many limitations, and for most types of cancer, effective early screening methods are still lacking, leading to generally low compliance. In recent years, with the advancement of molecular diagnostic techniques, liquid biopsy based on the methylation of circulating tumor DNA (ctDNA) has been a significant breakthrough in early cancer screening, demonstrating extensive potential applications. Currently, the methylation detection methods utilized for early cancer screening primarily include methylation-specific PCR (MSP), multiplex PCR amplification sequencing (Amp), and targeted methylation sequencing based on liquid-phase hybridization capture technology (Capture). MSP is only capable of qualitative detection of methylation and is generally used in the development of early screening products for a single type of cancer. Multiplex PCR amplification is applicable in the development of products for a single or multiple types of cancer, but its primer design is challenging. Nanodigmbio has launched the |iCaler targeted methylation sequencing solution, featuring an exclusive patented |iCaler liquid-phase hybridization capture system capable of completing the entire process within a single day, offering a simple and convenient operation. This solution captures the converted methylation libraries and uses a patented probe design scheme that covers all methylation states of the targets. Additionally, the hybridization capture system optimized for methylation is not only suitable for targeted enrichment with miniPanels but also applicable for capturing special regions like AT-rich sequences. Workflow gDNA/FFPE DNA cfDNA Duration

Index Index Perform Capture and Elution Perform Postcapture PCR & Cleanup μCaler Hybridization Targeted Capture μCaler NanoBlockers μCaler Panel μCaler Hybrid Capture Reagents v2 *The above schematic diagram is taking NadPrep Methyl Stubby Adapter (UDI) (with 10 nt Index) as an example. μCaler Targeted Methylation Sequencing Comprehensive Solution, featuring an exclusive patented μCaler hybridization capture system, enabling the completions of the entire process within same day with simple and convenient operations. This solution captures the converted methylation libraries using a patented...

μCaler Panel is a commercial or customized Panel (μCaler Custom Panel) based on Nanodigmbio's independent intellectual property rights with innovative scheme design. The panel is able to quickly find the binding position in the reaction system and further stabilize after multiple probes bind to the target region. If you hope to design personalized probes, please contact us (support@njnad.com) to get professional and personalized advice. Precise detection of large-scale CpG sites: Simultaneously assessment of multiple genes' CpG sites associated with various cancers, achieving precise...

Figure 1. Performance of μCaler targeted methylation sequencing with custom panels containing different scales of CpG sites. Human genomic DNA standards (Promega, G1471) were used to prepare pre-libraries using the NadPrep Methyl Library Preparation Module in conjunction with the NadPrep Methyl Stubby Adapter (UDI) Module (with 10 nt Index) and NadPrep DNA Methyl Bisulfite Conversion Mod- ule. Hybrid capture were completed with μCaler Methyl Cervical Cancer Panel (MCC) and μCaler Methyl Multiple Cancer Panel (MMC), followed by sequencing on Illumina Novaseq 6000, PE 150. For each sample, 1...

Fast and convenient operation Workflow Vacuum concentration Fast Wash Buffer 1 Fast Wash Buffer 1 Vacuum concentration Fast Wash Buffer 1 Fast Wash Buffer 1 Vendor T* (Fast) μCaler Highly efficient and stable MBT C base conversion rate (%) Target vovered (%) Vacuum concentration Pre hybridization Hybrid Capture Wash PCR Purification Figure 3. Capture performance of μCaler targeted methylation sequencing for mini panels. Pre-libraries were prepared using human genomic DNA standards (Promega, G1471), with 500 ng input per pre-library, and hybrid capture was completed with MBT, MMC and MPC,...

Target covered (%) Cost-effective sequencing Figure 4. Capture performance of μCaler targeted methylation sequencing under different mixed hybridization modes. Pre-libra- ries were prepared using human genomic DNA Standards (Promega, G1471), with 500 ng input per pre-library, and hybrid capture was performed with MCC. A. Target covered of CpG sites; B. CpG Methylation levels. Application example of clinical sample In cancer early screening cohort studies, various technical approaches are selected with the aim of validating methylation biomarkers screened across the whole genome, striving to...

Ordering Information Type Detail #Catalog Prep Module NadPrep Methyl Library Preparation Module, 96 rxn NadPrep Methyl Library Preparation Module, 24 rxn Methyl Library NadPrep DNA Methyl Bisulfite Conversion Module, 24 rxn NadPrep DNA Methyl Bisulfite Conversion Module, 96 rxn NadPrep Methyl Adapter (SI) Module Set A1 (for MGI), 24 rxn Methyl Adapter (SI) Module NadPrep Methyl Adapter (MDI) Module Set B1 (for MGI), 96 rxn NadPrep Methyl Stubby Adapter (UDI) Module Set A1 (with 10 nt Index), 24 rxn Hybrid Reagent Methyl Stubby Adapter NadPrep Methyl Adapter (MDI) Module Set A1 (for MGI), 24...