HiSNP-series Panel Introduction HiSNP Ultra Panel v1.0 is designed for analyzing genomic composition status with high resolution. This SNP skeleton-based panel can scan the whole genome with 50 Kb apart by targeting over 52,000 high MAF SNP sites. HiSNP Panel v1.0 is designed for analyzing genomic composition status with basic resolution. This SNP skeleton-based panel can scan the whole genome with 300 Kb apart by targeting over 9,000 high MAF SNP sites. High heterozygosity ratio: Comprehensive designing for Europeans, Africans, Americans and Asians (AF=0.05-0.95) Stable capture performance: GC balanced; unique flanking sequence (verified by WGS) Appropriate proportionality: Even distribution in whole genome; LD sites excluded Superior compatibility: Use alone or spike-in other CDS panels Application Product HiSNP Ultra Panel v1.0 HiSNP Ultra On-target rate HiSNP_MGI HiSNP_Illumina HiSNP Ultra_MGI HiSNP Ultra_Illumina HiSNP Ultra Fig 1. Capture performance of HiSNP-series Panel. DNA libraries were prepared using the NadPrep DNA Library Preparation Module (for MGI) and the NadPrep DNA Library Preparation Kit (for Illumina®), respectively. The A. mapping rate, on-target rate, B-C. coverage uniformity and consistency of HiSNP (HiSNP Panel v1.0) and HiSNP Ultra (HiSNP Ultra Panel v1.0) were exhibited, respectively. Target covered (%) Note: Sample type: Human genomic DNA, male (Promega, G1471). MGI platform: MGISEQ-2000 with PE100; Illumina platform: NovaSeq 6000 w

Target covered (%) On-target rate On-target rate Target covered (%) Fig 2. Capture performance of HiSNP Ultra Panel v1.0 with HRD standards. DNA libraries were prepared using the NadPrep DNA Library Preparation Module (for MGI) and the NadPrep DNA Library Preparation Kit (for Illumina®), respectively. The mapping rate, on-target rate, coverage uniformity and consistency of HiSNP Ultra Panel v1.0 were exhibited on A-B. MGI platform and C-D. Illumina platform. Note: GW3: GW-HRD-3 standards (Genewell); GW9: GW-HRD-9 standards (Genewell); GW12: GW-HRD-12 standards (Genewell). All standards are paired...

Fig 4. The coverage uniformity of HiSNP Ultra Panel v1.0 and Exome Plus Panel v2.0 in HRD standards. DNA libraries were prepared using the NadPrep DNA Library Preparation Kit (for Illumina®). The distribution of heterozygosis sites with ＞0.2x mean depth was calculated by splitting the genome into 3 Mb region. Compared with WES, the distribution of heterozygosis sites designed in HiSNP Ultra was more even in the range of whole genome. The heterozygosis sites of tumor sample were less than those of normal sample because of the Loss of Heterozygosity (LOH). Note: Sample type: HRD standards (Genewell)....