Nanodigmbio-HPVisual Panel instruction

Product catalog summary

Introduction
HPVisual Panel v1.0 is designed for the detection and genotyping of 226 human papillomavirus (HPV) subtypes, as listed by the International HPV Reference Center (2019). It covers entire genomes of 18 carcinogenic subtypes for comprehensive analysis, including viral integration into the host genome.

Application
This panel is specifically used for HPV detection.

Specification
The panel is available in two scales: 16 reactions (Catalog# 1001912) and 96 reactions (Catalog# 1001911), with a usage of 4 μL per reaction. Storage conditions are between -25°C and -15°C.

Instruction
The panel is optimized with NadPrep Hybrid Capture Reagents for targeted capture applications. Users should refer to specific manuals for Illumina® and MGI platforms. Hybrid capture reagents must be vortexed and centrifuged before use. Each hybridization reaction requires 13 μL of hybridization master mix and 4 μL of HPVisual Panel v1.0.

Recommendation
Various NadPrep products are recommended for use with the panel, including NanoBlockers and Hybrid Capture Reagents, with specific catalog numbers and storage conditions provided.

Attention
Reagent handling should be performed in a clean bench to avoid contamination. Tubes should be vortexed and centrifuged before opening, and repeated freezing and thawing should be avoided.

Statement
The HPVisual Panel v1.0 is for research use only and not for diagnostic procedures. All rights are reserved by Nanodigmbio Pte. Ltd.

Catalog excerpts

Nanodigmbio-HPVisual Panel instruction-1

We provide molecular reagents to serve precision medicine. HPVisual Panel v1.0 targets L1 gene segments of 226 human papillomavirus (HPV) subtypes listed by the International HPV Reference Center (2019) for HPV detection and genotyping. Moreover, the entire genomes of 18 carcinogenic subtypes are covered for more comprehensive analysis including viral integration into the host genome. This panel is used for HPV detection. Item Catalog# Scale Usage Storage Note: From June 1st, 2021, HPVisual Research Panel v1.0 has been renamed HPVisual Panel v1.0, and the catalog remains unchanged. • This panel has been optimized with NadPrep Hybrid Capture Reagents for targeted capture applications. Please refer to the user manual from Nanodigmbio (www.nanodigmbio.com). For Illumina® platforms, please refer to the Hybridization Capture of DNA Libraries (for Illumina®) _v2.3. For MGI platforms, please refer to the Hybridization Capture of DNA Libraries (for MGI)_v1.4. • The hybrid capture reagents need to be thoroughly vortex and centrifuge prior to use. Each hybridization reaction consists of 13 |iL of hybridization master mix and 4 |iL of HPVisual Panel v1.0. Note: Each hybridization master mix consists of 8.5 |iL of Hyb #1, 2.7 |iL of Hyb #2 and 1.8 |iL of Nuclease Free Water. • The HPVisual Panel v1.0 can either be used independently or as spike-in panel. Please contact us (support @njnad.com) for professional and specific recommendations. Service: [email protected] | Web: www.nanodigmbio.com

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Nanodigmbio-HPVisual Panel instruction-2

We provide molecular reagents to serve precision medicine. Catalog# Storage NadPrep Hybrid Capture Reagents NadPrep NanoBlockers (for Illumina®), 96 rxn NadPrep NanoBlockers (for MGI, SI), 96 rxn NadPrep NanoBlockers (for MGI, DI), 96 rxn NadPrep Hybrid Capture Reagents, 16 rxn NadPrep Hybrid Capture Reagents, 96 rxn NadPrep Amplification Primer Mix (for Illumina®), 96 rxn NadPrep M-Amplification Primer Mix (for MGI, SI), 96 rxn NadPrep M-Amplification Primer Mix (for MGI, DI), 96 rxn Attention • Perform all reagent handlings in the clean bench to avoid contamination. • Thoroughly vortex and...

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