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Immunoglobulin (IG) and T cell receptor (TR) genes comprise many different segment types, such as variable (V)，diversity (D) and joining (J) et al.，which are rearranged and brought together by V(D)J recombination during the differentiation and development of B-cell and T-cell sequences. This results in genetically different B-cells and T-cells, which provide an extremely diverse antigen recognition capability for the adaptive immune response. The sequence-level immune repertoire analysis plays an important role in the research of immune response and application of immunotherapy. Taking the advantage of the high error-tolerance by hybrid capture technology, IGTR Panel v1.0 was designed based on the entire pre-rearranged coding segments of B-cells and T-cells in the human genome, which can support the targeted capture sequencing of recombinant IG and TR coding and expression sequences at both the DNA and RNA levels. IGTR Panel v1.0 covers the entire IG and TR coding gene segments of the human genome, which can be used for the enrichment of segments before or after rearranged by V(D)JC recombination at the DNA level, and the enrichment of mature transcript at the DNACap Performance Target covered (%) Figure 1. Capture performance of IGTR Panel v1.0 in different DNA libraries. Libraries were prepared using NadPrep DNA Library Preparation Kit (for Illumina®) coupled with NadPrep UDI Adapter Kit (for Illumina®). IGTR Panel v1.0 were respectively used to complete hybridization capture, and sequenced on Illumina Novaseq 6000 with PE150. The BWA was used for alignment of raw reads to the reference genome (hg38). A. Mappability and On-target rate; B. Targeted covered. Note: The DNA_1-3 samples were: Ramos cell line DNA; Jurkat cell line DNA; Human Genomic DNA (Promega-male, G147
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Figure 2. Capture performance of IGTR Panel v1.0 in different RNA libraries. Libraries were prepared using NadPrep Total RNATo-DNA Module coupled with NadPrep DNA Library Preparation Kit (for Illumina®). IGTR Panel v1.0 were respectively used to complete hybridization capture, and sequenced on Illumina Novaseq 6000 with PE150. Analysis was performed using salmon. Note: The RNA_1-3 samples were: Ramos cell line total RNA; Jurkat cell line total RNA; Human Blood Peripheral Leukocytes Total RNA rRNA ratio Example Coverage of Transcript by RNACap Ramos Cell Line IGHV4-34_IGHJ6 Coverage CDR3...
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Ordering InformationProduct Catalog IGTR Panel v1.0, 16 rxn 1001942 Statement For research use only. Not for use in diagnostic procedures. Without the written permission of Nanodigmbio, no other individual or organization may reproduce, copy, edit, expand, reduce or translate the contents of this document in any form into other languages for any purpose. In case of improper use, Nanodigmbio Pte. Ltd. reserves all rights. Nanodigmbio Pte. Ltd. Service: sales@nanodigmbio.com | Web: www.nanodigmbio.com
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