NadPrep DNA Library Preparation Module v2Introduction NadPrep DNA Library Preparation Module v2 is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina® & MGI platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for applications including whole genome sequencing of 1-500 ng DNA and hybridization capture based targeted sequencing. Compared to the v1 version, the upgraded kit has improved the library conversion efficiency and better tolerance for low-quality samples. Consequently, it can enhance library complexity while maintaining high fidelity. Fast and Convenient Versatility • Complete the entire process of whole-genome library preparation • Flexible selection of multiple types of adapters to within 3 hr accommodate both MGI and Illumina platforms Flexible Compatibility Stable and Efficient • Compatible with input amounts ranging from 1 ng to 500 ng • Higher library yield • Compatible with various sample types such as gDNA, FFPE DNA, cfDNA, etc • Higher library conversion rate • Compatible with liquid-phase hybridization capture • Low GC bias MGI gDNA/FFPE DNA cfDNA Duration Illumina Fragmentation DNA [Option ^ Size Selection Adapter Ligation Library Amplification ^ptio^Optional step ^ NadPrep M-Adapter (DI) ^ NadPrep Universal Stubby Adapter

Stable and Efficient Library Yield Compatible with a wide range of input amounts A Figure 1. Library yield of NadPrep DNA Library Preparation Kit v2 for gDNA samples with different input amounts. The libraries were prepared by us- ing NadPrep DNA Library Preparation Module v2 coupled with NadPrep Universal Adapter (MDI) Module (for MGI) and NadPrep Universal Stubby Adapter (UDI) Module. A. Library yield (MGI); B. Library yield (Illumina). Note: gDNA samples are human genomic DNA standard (Promega, G1521). Compatible with various types of samples A Figure 2. Library yield of NadPrep DNA Library...

Compatible with different types of adapters NadPrep v2 Figure 3. Library yield of NadPrep DNA Library Preparation Kit v2 with di- fferent types of adapters. The libraries were prepared from different input samples by using NadPrep DNA Library Preparation Kit/Module (for Illumina®/MGI) (NadPrep v1) and NadPrep DNA Library Preparation Module v2 (NadPrep v2), coupled with NadPrep Universal Stubby Adapter (UDI) Module [Universal Stubby (UDI)], NadPrep Universal Adapter (MDI) Module (for MGI) [MDI], NadPrep BMI Adapter (MDI) Module (for MGI) [BMI], and NadPrep UMI Adapter Kit (with 10 nt MDI Universal...

Higher Library Complexity DCS211 Figure 6. Library complexity of NadPrep DNA Library Preparation Kit v2. 10 ng plasma cfDNA were prepared by using NadPrep DNA Library Preparation Module v2 coupled with NadPrep UMI Adapter Kit (with 10 nt Index). The capture was performed using a 2 Kb Panel with μCaler hybrid system. Sequencing plat- form: Illumina Novaseq 6000, PE150. For each sample, 1.0 Gb of data was randNadPrep v2 omly selected for data analysis, and filtering was performed based on Duplex Compatible with Liquid-phase Hybridization Capture NadPrep v1 NanOnco Plus Panel v3.0 NanOnco Plus Panel...

Table 1. Consistency of the allele frequencies with the nominal frequencies of the standards in captured data from the libraries prepared by NadPrep DNA Library Preparation Kit v2. Expected allele frequency / copy number Observed allele frequency / copy number The libraries were prepared by using NadPrep DNA Library Preparation Module v2 coupled with NadPrep Universal Stubby Adapter (UDI) Module, with 6 cycles of amplification. The capture was performed using NadPrep Hybrid Capture Reagents with the LungCancer Panel v1.0 (0.23 Mb), with a hybridization time of 16 hr. Sequencing platform: Illumina...

Type Product NadPrep UMI Adapter Kit Set A1 (with 10 nt Index), 24 rxn NadPrep UMI Adapter Kit Set B1 (with 10 nt Index), 96 rxn NadPrep UMI Adapter Kit Set B2 (with 10 nt Index), 96 rxn UM|/BM| Adapter NadPrep BMI Adapter (MDI) Module Set A1 (for MGI), 24 rxn Module NadPrep BMI Adapter (MDI) Module Set B1 (for MGI), 96 rxn NadPrep BMI Adapter (MDI) Module Set B2 (for MGI), 96 rxn NadPrep BMI Adapter (MDI) Module Set B3 (for MGI), 96 rxn NadPrep BMI Adapter (MDI) Module Set B4 (for MGI), 96 rxn Statement For research use only. Not for use in diagnostic procedures. Without the written permission...