DNA methylation, an epigenetic modification, is closely related to the gene expression and chromatin remodeling. Abnormal methylation status correlates to the occurrence and development of various diseases such as cancer. The precise identification of cytosine methylation status in genome plays a critical role in research and clinical areas, especially in precision oncology. In terms of analyzing methylation status, with the treatment of bisulfite or enzymes, the unmethylated cytosine can be converted to uracil, whereas the methylated cytosine remains unchanged. Afterwards, combined with high-throughput sequencing technology, the genomic methylation status can be analyzed massively with single nucleotide resolution. However, there are still cost and efficiency limitations for the analysis of whole genomes, the introduction of targeted enrichment technology can reduce the sequencing cost and improve the targeted analysis efficiency by improving the sequencing depth of regions of interest. Nanodigmbio offers an NGS total solution for capture-based methylation sequencing (Methyl-Seq) on both MGI sequencing platforms and Illumina sequencing platforms, including Methyl-Seq library preparation kit based on bisulfite or enzymatic conversion, methylation bisulfite conversion module, design & synthesis service of capture probes, hybridization reagents, and integrated analysis tools. Designed to help researchers to quickly and accurately analyze the methylation status of the genome, providing support for research and clinical diagnosis of related diseases.

Hybridization Capture Index Index Index Index Index Post Hybridization PCR & Cleanup Blocking & Targeted Capture *The above schematic diagram is taking NadPrep Methyl Stubby Adapter (UDI) (with 10 nt Index) as an example. NadPrep Methyl Library Preparation Module is a double-strand DNA methylation sequencing library preparation kit develo- ped for MGI & Illumina sequencing platforms. It supports multiple options for adapters selection, including: NadPrep Methyl Adapter (SI) Module (for MGI), NadPrep Methyl Adapter (MDI) Module (for MGI) and NadPrep Methyl Stubby Adapter (UDI) Module (with 10...

Hydrolytic Deamination Compatible with multiple types of samples and supports a wide range of input amounts Different types of adapter modules can be selected for matching according to the sequencing platform Efficient and stable cytosine conversion rate Excellent detection sensitivity of 5mC and 5hmC Convenient operation is conducive to automation implementation Library yield and conversion efficiency A 12 cycle 11 cycle 10 cycle 9 cycle 12 cycle 11 cycle 10 cycle 9 cycle Fig 1. Library yield and conversion efficiency of samples with different input amounts. Human Genomic DNA standards (Promega,...

Fold 80 base penalty Mappability NadPrep Average depth (rmdup) Target covered (%) Fig 2. Capture performance of samples with different methylation levels. 50 ng simulated samples with different methylation levels were prepared using NadPrep Methyl Library Preparation Module in combination with NadPrep Methyl Stubby Adapter (UDI) Module (with 10 nt Index) after bisulfite conversion. The pre-libraries were capture with a methylation demo panel (60 Kb). Sequencing was performed on Illumina Novaseq 6000, PE150, On-target rate was calculated by the number of reads. A. Mappability and On-target rate;...

Observed methylation level Theoretical methylation level Fig 3. Targeted sequencing performance of samples with different methylation levels. A. Comparison between theoretical methylation levels and detected methylation levels; B. CpG methylation levels in target regions. Ordering Information Type NadPrep Methyl Library Preparation Module, 24 rxn Methyl Adapter NadPrep Methyl Adapter (SI) Module Set A1 (for MGI), 24 rxn Methyl Adapter NadPrep Methyl Adapter (MDI) Module Set A1 (for MGI), 24 rxn NadPrep Methyl Library Preparation Module, 96 rxn NadPrep Methyl Adapter (SI) Module Set B1 (for MGI),...