Nanodigmbio-NadPrep rRNA Blocking Reagent
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NadPrep rRNA Fast Blocking Comprehensive Solution Background RNA sequencing (RNA-Seq) has become an indispensable and effective tool in basic biological research and disease diagnosis. It can not only detect differences in gene expression under different conditions but also reveal the diversity in RNA structure, such as alternative splicing, gene fusions, and single nucleotide mutations. In the total RNA of normal cells/tissues, ribosomal RNA (rRNA) typically accounts for over 80% of the content. The abundance of rRNA occupies a significant portion of sequencing capacity and resources, thus reducing the efficiency of detecting target transcriptome information. Therefore, the removal of rRNA impact is one of the crucial steps in RNA-Seq, contributing to improved accuracy, efficiency, and cost-effectiveness of research. This is particularly important for the detection of low-abundance transcripts, ensuring that sequencing resources are focused on the RNA molecules of interest. Traditional solutions involve enriching mRNA with Oligo dT magnetic beads or using bead-based and enzymatic methods to remove rRNA. These methods have some limitations, especially in terms of complex procedures, often requiring a significant amount of time. Therefore, Nanodigmbio has designed and developed the rRNA Fast Blocking Comprehensive Solution, aiming to achieve cost-effective and straightforward rRNA blocking. This solution requires only a single-step operation to rapidly and efficiently block rRNA, saving substantial time and effort. rRNA Blocking Reagent Random Primer RNA Fragmentation, rRNA Blocking & Random Primer Annealing 1st Strand cDNA Synthesis 2nd Strand cDNA Synthesis Double-stranded cDNA Cleanup

NadPrep rRNA Fast Blocking Comprehensive Solution, using NadPrep rRNA Blocking Reagent coupled with NadPrep Total RNA-To-DNA Module, seamlessly connects to NadPrep DNA Library Preparation Kit-series. This solution enables the rapid blocking of rRNA in total RNA, facilitating the detection of biological information in RNA samples, including gene expression, single nucleotide variations, alternative splicing, gene fusions, and more. NadPrep rRNA Blocking Reagent is a specialized processing module developed for high-throughput sequencing platforms to rapidly block rRNA in total RNA. This...

Compatible with Different Input Amounts for Various Sample Types A Cell line Normalized coverage were prepared using NadPrep rRNA Rapid Blocking Comprehensive Solution Figure 1. Performance of different input amounts across various types of samples using NadPrep rRNA Fast Blocking Comprehensive Solution rRNA ratio (%) Cell line (Human). A. rRNA ratio; B. No. of genes detected; C. Transcript coverage. Libraries connected to NadPrep DNA Library Preparation Kit-series. Sequencing platform: Illumina® Novaseq 6000, PE150, and 0.3 Gb of data per sample was randomly selected for data analysis....

Normalized coverage Figure 2. Performance of different input amounts of total RNA from zebrafish using NadPrep rRNA Fast Blocking Comprehensive Solution (Zebrafish). A. rRNA ratio; B. No. of genes detected; C. Transcript coverage. Libraries were prepared using NadPrep rRNA Rapid Blocking Comprehensive Solution (Zebrafish) connected to NadPrep DNA Library Preparation Kit-series. Relative positive in transcript from 5' to 3' end Compatible with Different Grades of FFPE Samples A rRNA ratio (%) Normalized coverage Relative position in transcript from 5' to 3' end Figure 3. Performance of...

Compatible with Third-party RNA Library Preparation Kits A rRNA ratio (%) Normalized coverage Relative positive in transcript from 5' to 3' end Figure 4. Performance of NadPrep rRNA Rapid Blocking Comprehensive Solution (Human) in combination with third-party RNA library preparation kits. A. rRNA ratio; B. No. of genes detected; C. Transcript coverage; D. Expression profile correlation. A total of 250 ng of K562 cell line RNA were processed using NadPrep rRNA Blocking Reagent in conjunction with NadPrep Total RNA-To-DNA Module, followed by library preparation using NadPrep DNA Library...

Compatible with MGI and Illumina Sequencing Platforms B rRNA ratio (%) Cell line Cell line Figure 5. Performance of NadPrep rRNA Fast Blocking Comprehensive Solution (Human) on dual sequencing platforms. A. rRNA ratio; B. No. of genes detected. With an input of 250 ng, libraries were prepared using NadPrep rRNA Fast Blocking Comprehensive Solution connected to NadPrep DNA Library Preparation Kit-series, coupled with NadPrep Universal Stubby Adapter (UDI) Module and NadPrep Universal Adapter (MDI) Module respectively. Sequencing platform: DNBSEQ-T7 and Illumina Novaseq 6000, PE150. rRNA...

Ordering Information Type Applicable Specie Human rRNA Fast Blocking Reagent Zebrafish Custom Total RNA-To-DNA NadPrep rRNA Blocking Reagent (Human), 24 rxn NadPrep rRNA Blocking Reagent (Zebrafish), 24 rxn NadPrep rRNA Blocking Reagent (Zebrafish), 96 rxn NadPrep Total RNA-To-DNA Module, 24 rxn NadPrep rRNA Blocking Reagent (Human), 96 rxn NadPrep rRNA Blocking Reagent (Custom) NadPrep Total RNA-To-DNA Module, 96 rxn *For Research Use Only. Nanodigmbio does not authorize the use of these products for clinical applications unless explicitly agreed to in writing. Nanodigmbio does not...