NanOnCT Panel NanOnCT Panel v1.0 is a compact panel designed for analysis of solid tumors and liquid biopsy. This panel involves a total number of 69 genes with selected regions covered. With an optimized probe design, this panel offers highly uniform coverage over targeted regions for both FFPE and cell-free DNA. Genes with coding regions covered AKT1 RB1 Genes with full coding regions shown in bold. Genes with selected introns covered ALK BRAF EGFR FGFR2 FGFR3 MET NTRK1 NTRK2 PDGFRA RET ROS1 Table 1. On-target rate for gDNA and cfDNA samples The DNA libraries were prepared using NadPrep library kits with 50 ng of human genomic DNA (Promega, G1512) and 10 ng of plasma cfDNA from a healthy donor, respectively. 1 M read pairs were subsampled for the analysis. On-target rates were calculated as the percentage of mapped read pairs that overlap with probe regions. On-target rate (%) On-target rate (%)

Coverage Uniformity Fig 1. Improved design for cfDNA capture. The optimized design of the NanOnCT Panel v1.0 showed improved coverage uniformity for cfDNA libraries on both Illumina® and MGI platforms. A|ieie frequency Observed frequency by Vardict by digital PCR HiSeq X Ten DNBSEQ-G400 Allele frequency by digital PCR (%) Fig 2. Evaluation of the NanOnCT Panel v1.0 on the analysis of SNVs and indels. DNA libraries were prepared from Onco SNV Multiplex 1-25% gDNA (GeneWell, GW-OGTM004) using NadPrep library kits. The enriched libraries were sequenced either on HiSeq X Ten (PE150) or DNBSEQ-G400...