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Nanodigmbio-NGS HRR Panel

Nanodigmbio-NGS HRR Panel

Product catalog summary

Introduction
HRR Panel v1.0 is designed to target the entire coding sequences of 35 genes related to DNA Homologous Recombination Repair (HRR). It can be utilized independently for analyzing HRR gene mutations or in conjunction with the HiSNP-series panel to assess Homologous Recombination Deficiency (HRD) by examining genomic instability alongside HRR-related gene mutations.

Gene List
The document lists several genes involved in HRR, including ATM, ATR, BARD1, BLM, BRCA1, BRCA2, and others, which are crucial for understanding the genetic basis of HRR.

Capture Performance
Figures 1 and 2 illustrate the capture performance of the HRR Panel v1.0. The performance metrics include mapping rate, on-target rate, and coverage uniformity. The DNA libraries were prepared using the NadPrep DNA Library Preparation Kit and sequenced on the NovaSeq 6000 platform.

Variant Analysis
Variant analysis was conducted using Vardict, with the sequencing performed on the NovaSeq 6000 platform. The analysis focuses on the observed versus expected allele frequencies for the HRR-related genes.

Spike-in Capture Performance
Figure 3 demonstrates the spike-in capture performance when HRR Panel v1.0 is used with the HiSNP Ultra Panel v1.0. The performance metrics include mapping rate, on-target rate, and coverage consistency. The samples used were human genomic DNA and Onco gDNA.

Ordering Information
The product catalog number for HRR Panel v1.0 is 1001931, and it is available in 96 reactions per kit.

Additional Information
The document specifies that the HRR Panel is for research use only and not for diagnostic procedures. Contact information for Nanodigmbio Pte. Ltd. is provided for further inquiries.

Catalog excerpts

HRR PanelIntroduction HRR Panel v1.0 targets whole coding sequences of 35 DNA Homologous recombination repair (HRR) related genes. It can be used alone for HRR gene mutation analysis, as well as spike-in to HiSNP-series panel for Homologous Recombination Deficiency (HRD) assessment by analyzing genomic instability along with HRR-related gene mutation. FANCD2 FANCE FANCF FANCG FANCI FANCL FANCM MRE11 NBN PALB2 RAD50 RAD51 Depth of coverage (mean = 100) Fig 1. Capture performance of HRR Panel v1.0. DNA libraries of human genomic DNA, male (Promega, G1471) were prepared by using the NadPrep DNA Library Preparation Kit (for Illumina®), and captured by using HRR Panel v1.0. The A. mapping rate, on-target rate, coverage uniformity, and B. coverage consistency were exhibited, respectively. Sequencing platform is NovaSeq 6000 with PE150. Fig 2. Variant analysis by HRR Panel v1.0. DNA libraries of Onco gDNA (Genewell, GW-OGTM800) were prepared by using the NadPrep DNA Library Preparation Kit (for Illumina®), and captured by using HRR Panel v1.0. The variant analysis was performed by Vardict. Sequencing platform is NovaSeq 6000 with PE150. Expected allele frequency (%)

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Spike-in Capture Performance A Target covered (%) On-target rate Fig 3. Spike-in capture performance of HRR Panel v1.0. DNA libraries were prepared by using the NadPrep DNA Library Preparation Kit (for Illumina®), and captured by using HRR Panel v1.0 and HiSNP Ultra Panel v1.0 with equimolar. The A. mapping rate, on-target rate, B & C. coverage uniformity and consistency were exhibited, respectively. Sample type: Human genomic DNA, male (Promega, Target covered (%) G1471), Onco gDNA (Genewell, GW-OGTM800), and Onco SNV Multiplex wildtype gDNA (Genewell, GW-OGTM005). Sequencing platform is NovaSeq...

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