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Arthur™ - 4 Pages

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Arthur™

Catalog excerpts

Image based cell analyzer Experience Accuracy! Cell Counting & Analyzing with Arthur™ - Accurate cell counting & analysis - Various & fast analysis assay tools - Rapid setup, user friendly interface

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Image based cell analyzer Arthur™, Image based cell analyzer is 3-channel (bright field, green fluorescence, red fluorescence) desktop image analyzer that allows users to perform assays for cells in suspension, including GFP and RFP expression, apoptosis, cell viability (live, dead, and total cells), cell cycle, and cell counting assays using state-of-the-art optics. It is compatible with a wide variety of eukaryotic cells. Depending on complexity of the assay and number of fields captured, it takes 10 seconds to 2 minutes that Arthur™ counts for a typical assay with only 25 uL of sample...

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Image based cell analyzer Cell viability assay Cell line: Jurkat Figure 1. Comparison of cell viability in two platforms Heat shocked cells were stained with Propidium Iodide (PI) and percent viable cells of the total population were analyzed by Arthur™ (A) and competitor's product F (B) with two different cell types, Jurkat and U-2 OS. In all cases, the fluorescence threshold setting was confirmed visually In the image of the Arthur™. For each cell line, viability results from the Arthur™ were consistent with the results obtained from the competitor's product F. Figure 2. Comparison of GFP...

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Annexin V apoptosis analysis Figure 3. Arthur™ allows confirmation of apoptosis assay using the visual display of the samples Following 18 hour-treatment of Staurosporine, HeLa cells were stained with apoptosis kit and subjected to Arthur™ and competitor's product F. The apoptosis kit contains an annexin V-Alexa Fluor® 488 conjugate and PI to differentiate live (annexin V-negative/PI-negative), dead (Pl-positive), and apoptotic (annexin V-positive/PI-negative) cells. Using Arthur™, 20 % of the cell population were measured as live, 38 % apoptotic, and 42 % dead, confirming that the Arthur™...

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