BACE-1 Data Sheet Catalog Number: RA21010 Product Type: Rabbit Polyclonal IgG Immunogen Sequence: Synthetic peptide Host: Rabbit Species Reactivity Human, Mouse, and Rat Format: Lyophilized powder Applications: Immunohistochemistry: 5-10 ug/ml Immunofluorescence: 5-15 ug/ml Western Blot: 1 ug/ml Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Storage: The product can be stored as supplied for up to 12 months at 2°C-4°C. After reconstitution, aliquot and store at -20°C for higher stability or at 4°C with an appropriate antibacterial agent. Avoid freeze thaw-cycles. Application Notes Description/Data: BACE1, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. BACE-1 is a member of the peptidase A1 protein family and is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. In brains of patients with Alzheimer's disease, BACE-1 is detected in reactive astrocytes, suggesting that astrocyte activation may play a role in the development of Alzheimer's disease. Images: Immunohistochemical detection of BACE-1 in astrocytes in rat cortex. Rat brain was fixed with 4% formaldehyde and cut into 10 gm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 gg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue). Neuromics’ reagents are for in vitro and certain non-human in vivo experimental use only and not intended for use in any human clinical investigation, DIAGNOSIS, PROGNOSIS, OR TREATMENT. THE ABOVE ANALYSES ARE MERELY TYPICAL GUIDES. THEY ARE NOT TO BE CONSTRUED AS BEING SPECIFICATIONS. ALL OF THE ABOVE INFORMATION IS, TO THE BEST OF OUR KNOWLEDGE, TRUE AND ACCURATE. HOWEVER, SINCE THE CONDITIONS OF USE ARE BEYOND OUR CONTROL, ALL RECOMMENDATIONS OR SUGGESTIONS ARE MADE WITHOUT GUARANTEE, EXPRESS OR IMPLIED, ON OUR PART. WE DISCLAIM ALL LIABILITY IN CONNECTION WITH THE USE OF THE INFORMATION CONTAINED HEREIN OR OTHERWISE, AND ALL SUCH RSKS ARE ASSUMED BY THE USER. WE FURTHER EXPRESSLY DISCLAIM ALL WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.-V2/08/2012