TRPM2 Data Sheet Host: Rabbit Immunogen Sequence: Synthetic peptide made to a C- terminal portion of the rat protein (within residues 1430-1508). [Swiss-Prot# Q5G856] Product Type: Affinity Purified Antibody Species Reactivity: Human, Mouse glycerol with .01% sodium azide. Concentration: 1.18 mg/ml. Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator. Storage: Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Repeated freeze/thaw cycles compromise the integrity of the antiserum. Note: This antibody is specific for isoform 1, or the long isoform (TRPM2-L), of TRPM2. Protein localized in cell membrane. Designed for Immunohistochemistry and Western Blot (band at app.181kDa) Image: TRPM2 (red) and GFAP (green) staining of rat hippocampus. Nclei staining is blue. Image courtesy of Dr. Ji-Zhong Bai, The University of Auckland, New Zealand. Neuromics’ reagents are for in vitro and certain non-human in vivo experimental use only and not intended for use in any human clinical investigation, DIAGNOSIS, PROGNOSIS, OR TREATMENT. THE ABOVE ANALYSES ARE MERELY TYPICAL GUIDES. THEY ARE NOT TO BE CONSTRUED AS BEING SPECIFICATIONS. ALL OF THE ABOVE INFORMATION IS, TO THE BEST OF OUR KNOWLEDGE, TRUE AND ACCURATE. HOWEVER, SINCE THE CONDITIONS OF USE ARE BEYOND OUR CONTROL, ALL recommendations or suggestions are made without guarantee, express or implied, on our part. We disclaim all liability in connection with the use of THE INFORMATION CONTAINED HEREIN OR OTHERWISE, AND ALL SUCH RSKS ARE ASSUMED BY THE USER. WE FURTHER EXPRESSLY DISCLAIM ALL WARRANTIES OF MERCHANTABILITY and FITNESS FOR A PARTICULAR PURPOSE. V2-01/13 Neuromics Antibodies • 5325 West 74th Street, Suite 8 • Edina, MN 55439 phone 866-350-1500 • fax 612-677-3976 • e-mail [email protected]

Image: Detection of TRPM2. Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured). Photo courtesy of Dr. Paco Herson, Oregon Health & Science University. FOR RESEARCH USE ONLY NEUROMICS’ REAGENTS ARE FOR IN VITRO AND CERTAIN NON-HUMAN IN VIVO EXPERIMENTAL USE ONLY AND NOT INTENDED FOR USE IN ANY HUMAN CLINICAL INVESTIGATION, DIAGNOSIS, PROGNOSIS, OR TREATMENT. THE ABOVE ANALYSES ARE MERELY TYPICAL GUIDES. THEY ARE NOT TO BE CONSTRUED AS BEING SPECIFICATIONS. ALL OF THE ABOVE INFORMATION IS, TO THE BEST OF OUR KNOWLEDGE, TRUE AND...