Multiphoton Confocal Microscope A1 MP+/A1R MP+ i m MP+ Multiphoton Confocal Microscope

The A1 MP+ and A1R MP+ multiphoton confocal microscopes provide faster and sharper imaging from deeper within living organisms, extending the boundaries of traditional research techniques in biological sciences. • Deep specimen imaging with ultrasensitive GaAsP non-descanned detectors (GaAsP NDDs) located close to the back aperture of the objective lens. In particular, 1300 nm-compatible episcopic GaAsP NDDs for upright microscopes allow deep in vivo imaging of mouse brains up to 1.4 mm. •A 1300 nm-compatible A1R MP+ scan head which is capable of simultaneous excitation with two wavelenghts is...

Ultra-deep imaging of living specimens Ultrasensitive GaAsP NDDs allow clear in vivo imaging in deeper areas than ever before and are powerful enough to analyze fast dynamics, such as the activation of neurons, in living specimens. In addition to the 1080 nmcompatible model, a 1300 nm-compatible model GaAsP NDD for upright microscopes is also available, enabling deep imaging of up to 1.4 mm when combined with a 1300 nm-compatible A1R MP+ scan head. Deep brain imaging in in vivo mouse with 1300 nm-compatible GaAsP NDD In vivo imaging of an anesthetized YFP-H mouse (4-week-old) via open skull method....

Simultaneous excitation imaging with two-wavelength IR laser The A1R MP+ is now available for two-wavelength simultaneous IR excitation. Combining a system with a femtosecond IR pulse laser with two wavelength simultaneous output (main tunable output of 700 – 1300 nm and auxiliary fixed output of 1040 nm) enables the simultaneous excitation and imaging of two different dyes in the deep areas within living cells. Two wavelength simultaneous excitation imaging of a zebrafish Three dimensional images of 1 dpf zebrafish transgenic line, Tg[h2afv:GFP; EF1α : mCherry-zGem]. After breeding under Phenyltiourea...

Channel unmixing Fast multiphoton imaging, powerful enough for in vivo imaging The Nikon resonant scanner is equipped with A1R MP+ and capable of high-speed 420 fps (512 x 32 pixel) imaging. Unique to this design is a resonant scan mirror capable of imaging full fields of view at much higher speeds than traditional galvano scanners. Nikon's optical pixel clock system, which monitors the position of the resonant mirror in real time, adjusts the pixel clock to ensure more stable, geometrically correct and more evenly illuminated imaging, even at high speeds. This enables the successful visualization...

Channel unmixing With multiphoton excitation, fluorophores have a considerably broader profile of the absorption spectra than with single photon excitation. Therefore, simultaneous excitation of multiple fluorophores with a single excitation wavelength is possible. Additionally, the wavelength of a pulsed laser for multiphoton excitation can be changed and the user can select a suitable and well-balanced wavelength for the excitation of multiple fluorophores. A1 MP+/A1R MP+ NDD and channel unmixing technology enable the user to clearly isolate multiple fluorophores and obtain information on the...

Channel unmixing Multiphoton imaging gallery Four-color imaging of human colon cancer cells in in vivo Three-dimensional volume rendering of implanted subcutaneous tumor of HCT116 expressing Fucci. The cell cycle of tumor cells and the environment (collagen fiber and vessels) are visualized. Upper right, only collagen fiber and vessels are shown. Red: Fucci mkO2/cancer cell Green: Fucci mAG/cancer cell Cyan: SHG/collagen fiber Magenta: Qtracker655/neovascular vessels Objective: CFI Plan Fluor 20xA MI Excitation Wavelength: 940 nm Photographed with the cooperation of Drs. Yoshinori Kagawa and...

SHG image of the brain surface of a mouse The neocortex of an H-line 5-week-old mouse was studied with the open skull method. The SHG signals from dura mater and EYFP fluorescence signals were simultaneously acquired using the NDD. EYFP fluorescent image SHG image of the dura mater Overlay image Excitation wavelength: 950 nm Objective: CFI75 Apochromat 25xW MP (NA 1.10 WD 2.0) Photographed with the cooperation of: Dr. Takeshi Imamura, Graduate School of Medicine, Ehime University Drs. Yusuke Oshima and Shigenori Nonaka, National Institute for Basic Biology Drs. Terumasa Hibi, Ryoshuke Kawakami...

A1 MP+/A1R MP+ achieve the most advanced multiphoton imaging Deep in vivo imaging with super high-sensitive GaAsP NDD Light scattering is the main issue when doing deep imaging in living specimens, since it compromises the acquisition of bright images with a high S/N ratio. To overcome this issue, A1 MP+/A1R MP+ provides various functions in its high sensitivity detector unit for multiphoton imaging: • he NDD (non-descanned detector) is located as close as possible to a living specimen to detect the maximum T amount of scattered emission signals from deep within the specimen. • he GaAsP NDD is...

Nikon’s high-NA objectives are ideal for multiphoton imaging High-NA objectives that effectively correct chromatic aberrations over a wide wavelength range from ultraviolet to infrared have been developed. Transmission is increased through the use of Nikon’s exclusive Nano Crystal Coat technology. In particular, the CFI Apochromat 25xW MP/MP1300 objective lenses provide the industry’s highest numerical aperture of 1.10, while still maintaining a 2.0 mm working distance. They also have a collar that corrects spherical aberrations depending on the depth of the specimen, and a 33° manipulator pipette...

The A1 MP+ is equipped with a galvano (non-resonant) scanner for high-resolution imaging. The A1R MP+ is a hybrid scan head that incorporates both galvano and ultrahigh-speed resonant scanners. The A1R MP+ allows imaging and photoactivation at the ultrafast speeds necessary for revealing cell dynamics and interaction. The A1R MP+ is available in two types - a 1080 nm-compatible type and a 1300 nm-compatible type capable of deeper imaging. The A1 MP+/A1R MP+ galvano scanner enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with its newly developed scanner driving and sampling...