Multiphoton Confocal Microscope A1 MP+/A1R MP+ NIKON CORPORATION Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100-8331, Japan www.nikon.com/ Multiphoton confocal microscope

The A1 MP+/A1R MP+ multiphoton confocal microscopes provide faster and sharper imaging from deeper within living organisms, extending the boundaries of traditional research techniques in biological sciences. •Ultrahigh-speed imaging up to 420 frames per second (fps) (512 x 32 pixels) with multiphoton imaging using A1R MP+’ high efficiency optics and resonant scanner. • Deep specimen imaging with high-sensitive non-descanned detectors (NDD) located close to the back aperture of the objective lens. A new model of an ultrasensitive gallium arsenide phosphide (GaAsP) NDD for upright microscopes that...

Ultra-deep imaging of living specimens The ultrasensitive GaAsP NDD allows clear in vivo imaging in deeper areas than ever before and is powerful enough to analyze the mechanisms, such as brain neurons, of living specimens. In addition to a model compatible with a wavelength of 1080 nm, there is a new one for upright microscopes that is compatible with a wavelength of 1300 nm. This new NDD enables deep imaging up to 1.4 mm in combination with a newly developed scanning head A1R MP+ that is compatible with a wavelength of 1300 nm. Mouse brain in vivo dual color imaging with the GaAsP NDD compatible...

Channel unmixing Fast multiphoton imaging, powerful enough for in vivo imaging Channel unmixing The Nikon resonant scanner is capable of high-speed 420-fps imaging, the world’s fastest for a multiphoton microscope using point scanning technology. Unique to this design is a resonant scan mirror capable of imaging full fields of view at much higher speeds than traditional galvano scanners. Nikon's optical pixel clock system, which monitors the position of the resonant mirror in real time, adjusts the pixel clock to ensure more stable, geometrically correct and more evenly illuminated imaging even...

Channel unmixing Multiphoton imaging gallery Four-color imaging of human colon cancer cells in in vivo SHG image of the brain surface of a mouse Three-dimensional volume rendering of implanted subcutaneous tumor of HCT116 expressing Fucci. The cell cycle of tumor cells and the environment (collagen fiber and vessels) are visualized. Upper right, only collagen fiber and vessels are shown. The neocortex of an H-line 5-week-old mouse was studied with the open skull method. The SHG signals from dura mater and EYFP fluorescence signals were simultaneously acquired using the NDD. Red: Fucci mkO2/cancer...

A1 MP+/A1R MP+ achieve the most advanced multiphoton imaging Standard NDD Nikon’s high-NA objectives are ideal for multiphoton imaging The fluorescence emissions from deep within a specimen are highly scattered in multiphoton excitation, and therefore the conventional detector using a pinhole cannot provide bright fluorescent images. The episcopic NDD in the A1 MP+/A1R MP+ is located close to the back aperture of the objective to detect the maximum amount of scattered emission signals from deep within living specimens. The use of this four-channel detector in combination with special spectral...

Two types of scanning head enable high-speed, high-quality imaging A1 MP+ is equipped with a galvano (non-resonant) scanner for high-resolution imaging. A1R MP+ is a hybrid scanning head that incorporates both galvano and ultrahigh-speed resonant scanners. A1R MP+ allows imaging and photoactivation at ultrafast speeds necessary for revealing cell dynamics and interaction. Ultrafast photoactivation and imaging High-resolution imaging Photoactivation during fluorescence imaging can be conducted using both galvano and resonant scanners. Two types of A1R MP+, the conventional 1080 nm type and the...

Key Nikon innovations for improving image quality Enhanced spectral detector The best image quality is achieved by an increased light sensitivity resulting from comprehensive technological innovations in electronics, optics and software. Nikon’s original spectral performance is even further enhanced in the A1 MP+ series, allowing high-speed spectral acquisition with a single scan. In addition, advanced functions, including real-time unmixing, are incorporated. Low-angle incidence dichroic mirror With the A1 MP+ series, the industry’s first low-angle incidence method is utilized on the dichroic...

Intuitive, easy-to-use software for multiphoton imaging NIS-Elements C Acquisition and Analysis software Simple operations common with Nikon confocal microscopes Channel unmixing function Microscope control •All necessary operations for image capture are displayed in one window. •Lasers and detectors for visible laser excitation can be switched simply by selecting fluorescent probe to be used. •One-touch switching of high speed resonant scanner and high-resolution galvano (non-resonant) scanner •Simultaneous photoactivation with high speed imaging is possible with visible laser excitation. Nikon's...

System diagram ^ Laser Unit for Multiphoton Microscopy ^ Chameleon Vision II MaiTaiHP/eHP InSight DeepSee*1 Laser Unit for Confocal Microscopy ^ LU-LR 4-laser Power Source Rack ^ Detector Unit for Confocal Microscopy^ A1-DUS Spectral Detector Unit Filter Cubes ^ Incident Optical Unit ^ ^ Scan Head and Controller ^ Scan Head Detector Unit for Multiphoton Microscopy Diascopic Non-descanned Episcopic Non-descanned Detector (Standard/ (Standard/ Filter Cubes *1 When using a scan head for 1300 nm *2 When using Spectral Detector Unit. *3 Dedicated adapter is required depending on microscope model....

Laser Chiller for Multiphoton Microscopy Incident Optical Unit Incident Optical Unit Laser for Multiphoton Microscopy Laser Controller for Multiphoton Microscopy Laser Controller for Multiphoton Microscopy Scan Head Scan Head Non-Descanned Detector Vibration Isolated Table Non-Descanned Detector 4 Detector Unit, Spectral Detector Unit, Controller Vibration Isolated Table 4 Detector Unit, Spectral Detector Unit, Controller Laser Chiller for Multiphoton Microscopy 4-laser Module A, 4-laser Power Source Rack Approx. 2750 4-laser Module A, 4-laser Power Source Rack Laser for Multiphoton Microscopy...