Bio Station IMQ
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Time Lapse Imaging System BioStation IM-Q Time Lapse Imaging System NIKON CORPORATION Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100-8331, Japan www.nikon.com/ Combining a microscope, incubator and high-sensitivity digital camera into one powerful system

The perfect solution for long-term, time-lapse, live-cell imaging The BioStation IM-Q incorporates a microscope, an incubator and a high-sensitivity cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition. Moreover, focus drift caused by thermal change and mechanical instability has been minimized, and fail-proof data acquisition is possible even during lengthy time-lapse imaging. The BioStation IM-Q eliminates the need for a darkroom for fluorescence imaging, meaning it...

ICell-friendly environmental control Cell culture and image capture functions are beautifully integrated. No complex setup or alignment procedures that conventional time-lapse observation systems require are necessary. The vibration-proof and heat-insulated structure enables stable image acquisition even over long periods. Red LED illumination is used for phase contrast imaging. The fluorescence illuminator shutter opens only when capturing images, therefore reducing phototoxicity of live cells. The standard specimen chamber can be sterilized in an autoclave (a hightemperature,...

Simple and easy operation IOperation from a PC BioStation IM-Q provides fully motorized control from a PC, allowing users who are not accustomed to operating a microscope or camera to easily conduct time-lapse imaging. The user only has to set the culture dish in place and program the image recording. Everything from cell management to timelapse imaging is automatically managed and accomplished. Culture dish setup Setting with intuitive GUI Live image location indicator: imaging positions can be selected by clicking on the live image Environmental setup ❶ Set live image location with XYZ...

IImage file output at desired Z-axis planes Images at different Z-axis planes can be selected from the captured Z-stack images at each time point and assembled into a seamless movie file. This is optimal for imaging a specimen in which the observation point moves along the Z-axis direction, such as with cell division. IStreaming function Rapid motion changes such as cardiomyocyte beats are captured by high-speed 10 fps imaging at user-defined time intervals. User-defined time interval IErgo controller IWidefield display An ergo controller allows X, Y and Z directional movement with an...

Two kinds of analysis software are available for intended use. (Option) Imaging software NIS-Elements Ar Nikon’s proprietary imaging software NIS-Elements Ar allows multi-dimensional image capture, image processing, and data management and analysis of up to 6D. Its intuitive interface simplifies workflow and contributes to archiving and searching of large numbers of Z-stack sequence image files. It also offers diverse optional plug-ins to support sophisticated, cutting-edge research applications. Cell counting Haze and blur of an image that can occur when capturing a thick specimen or a...

Automated changeover allows each of the four culture wells to be imaged. Observation of four different conditions is possible in a single time-lapse experiment, facilitating comparative analyses. Operation screen I Specialized Hi-Q4 culture dish that enables multi-sample observation This new 35 mm culture dish is divided into four parts and has an incorporated plane parallel top plate. The plate prevents light path distortion by the meniscus, which is the curve at the air-water interface, and enables high-quality phase contrast observation. The observation points in the quadrant of the...

Fraunhofer Institute for Biomedical Engineering Department Biophysics & Cryotechnology (Prof. Dr. H. Zimmermann and M. Gepp, M.Sc.), Fraunhofer IBMT, which conducts cell management and technological development of long-term storage, freezing and thawing, introduced 10 BioStation IM into their laboratory to efficiently conduct the experiments and is researching to improve culture conditions by simultaneously running multiple experiments with BioStation IM. Research papers stating data obtained with BioStation IM- • Karine Gousset, Edwin Schiff, Christelle Langevin, Zrinka Marijanovic, Anna...