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C2 Plus

Product catalog summary

Overview of the C2+ Confocal Microscope
The C2+ confocal microscope is a key model in Nikon's product line, designed for laboratory use with advanced imaging capabilities. It features high-efficiency scan heads and detectors for superior confocal images, supporting high-speed galvano scanning up to 100 fps. This allows for precise capture of dynamic events such as cardiac muscle motion. The system can simultaneously acquire three fluorescent channels plus DIC in a single scan. The C2si+ system includes a dedicated spectral detector for simultaneous acquisition of 32 spectral channels and efficient unmixing of overlapping data.

High-Speed Imaging and Pixel Shift Correction
The C2+ system's galvanometer-based high-speed scanning is ideal for imaging fast, dynamic events in live cells. It includes a pixel shift correction mechanism to maintain high-quality images during fast bi-directional scanning, exemplified by imaging rat cardiac muscle cells labeled with Fluo-8 AM and MitoTracker® Orange.

Image Quality and Optical Design
Nikon's optics deliver bright, sharp images at long working distances. The C2+ uses high-precision mirrors and circular pinholes to reduce noise and enhance image quality, supporting high-speed imaging at 8 fps (512 x 512 pixels) and 100 fps (512 x 32 pixels).

High-Performance Objectives
The CFI Apochromat λS Series corrects chromatic aberration from ultraviolet to infrared, suitable for multicolor imaging. Nano Crystal Coat technology enhances lens transmission properties. The CFI Apochromat TIRF Series offers high numerical aperture (NA) objectives for high-resolution imaging.

Imaging Software and Multimode Capability
NIS-Elements software provides image processing and analysis functions, supporting various imaging methods like confocal, widefield, and TIRF. It offers intuitive operation and data extraction capabilities with a simple GUI for setting parameters and spectral analysis.

True Spectral Imaging with C2si+
The C2si+ system includes a standard fluorescence detector and a spectral detector for accurate spectral data acquisition. It captures wavelength changes in true color and unmixed overlapping spectra, featuring a Diffraction Efficiency Enhancement System (DEES) for increased diffraction efficiency and supports effortless fluorescence unmixing with a built-in spectral database.

Specifications
The C2si+ is equipped with two detectors: a standard three-channel fluorescence detector and a dedicated spectral detector. It captures spectral data over a 320 nm-wide wavelength range in a single scan, minimizing cell damage. The system includes a laser shielding mechanism and is compatible with various laser selections.

High-Performance Imaging Software
NIS-Elements software offers image processing, analysis, and data extraction capabilities, supporting various imaging methods and providing an intuitive interface for controlling Nikon microscopes and third-party devices.

Multimode Capability
The software supports confocal, widefield, TIRF, and photoactivation imaging methods, allowing users to control different systems with a common interface.

True Spectral Imaging
The C2si+ allows accurate spectral data acquisition and unmixes overlapping spectra. It features a high diffraction efficiency system (DEES) that enhances overall diffraction efficiency by converting polarizing light beams.

Fluorescence Unmixing
The system can unmix closely overlapping fluorescence spectra and eliminate auto-fluorescence. It includes a database of spectral data for reference spectra and allows users to add new labels.

Correction Techniques
Three correction techniques ensure accurate spectral data: interchannel sensitivity correction, spectral sensitivity correction, and correction of spectral transmission in optical devices.

High-Efficiency Fluorescence Transmission
Proprietary anti-reflective coatings on fluorescence fibers and detector surfaces reduce signal loss, achieving high optical transmission.

FRET Analysis
True spectral FRET analysis allows three-dimensional analysis with high signal-to-noise ratio and spatial resolution. It enables real-time detection of spectral changes and ratio analysis of fluorescence signals.

Laser Units
The C2+/C2si+ confocal microscopes offer two types of laser units: a compact model with pre-installed lasers and a flexible model supporting up to eight lasers and seven output fibers.

Overview of Laser Systems
The document details the LU-NV laser system and the AZ-C2+ macro confocal microscope system, designed for advanced imaging applications, including confocal microscopy, TIRF, and photoactivation.

Laser Units
The LU-NV series supports multiple laser applications with up to eight different lasers and seven output fibers. The LU-N4/N4S and LU-N3 models offer compact solutions with four and three lasers, respectively. Key features include high-power lasers, individual laser control, pre-aligned optical axes, and a monolithic laser combining system to prevent alignment shifts.

Specifications
The LU-N4/N4S models include lasers at 405 nm, 488 nm, 561 nm, and 640 nm, while the LU-N3 includes 405 nm, 488 nm, and 561 nm. The LU-NV series can mount lasers at various wavelengths, including 405 nm to 647 nm, with varying output powers.

AZ-C2+ Macro Confocal System
This system enables high-resolution imaging of large specimens (>1 cm) with a combination of low and high magnification lenses. It supports continuous imaging from macro to micro levels and allows deep imaging of specimens, capturing fluorescence signals efficiently.

Imaging Capabilities
The AZ-C2+ system offers time-lapse observation and high magnification imaging, providing clear and sharp images of single cells. It supports a wide field of view and high-resolution optical sections in a single scan.

Applications
The systems are suitable for various biological imaging applications, including observing cell populations, embryo development, and cancer cell imaging. The document includes examples of specimen imaging, such as chick embryos and human cancer cells.

Technical Specifications
The document details the dimensions, weight, and laser types for each unit, along with scanning capabilities and detector specifications for the confocal systems.

Specifications
The document outlines the specifications of a spectral imaging confocal microscope system. It includes a spectral detector with 32 channels covering wavelengths from 400-750 nm, with resolutions of 2.5 nm, 5 nm, and 10 nm. The field of view is a square inscribed in an 18 mm circle, and the image bit depth is 12 bits. Compatible microscopes include inverted, upright, fixed stage, and multi-purpose zoom microscopes.

Procedures and Software
The system uses NIS-Elements C software for display, image processing, and analysis, supporting 2D/3D/4D analysis, time-lapse, 3D volume rendering, spectral unmixing, and more. Applications include FRAP, FLIP, FRET, and photoactivation.

Control Computer Requirements
The control computer should run on Microsoft Windows 7 Professional 64 bit SP1, with an Intel Xeon E5-1630v3 CPU or higher, 4 GB memory, and a 146 GB or higher hard disk. A dual monitor setup with a resolution of 1600 x 1200 or higher is recommended.

Dimensions and Weight
The C2+/C2si+ scan head measures 145 x 236.5 x 70 mm and weighs approximately 2 kg. The controller is 225 x 404 x 382 mm, weighing about 12 kg. The standard detector is 225 x 154 x 224 mm, weighing approximately 5 kg, while the spectral detector is 289 x 323 x 593.5 mm, weighing about 23 kg.

Laser Units
The system supports various laser units, including LU-N3 and LU-N4/N4S, with wavelengths ranging from 405 nm to 647 nm. The LU-NV series allows for additional wavelengths.

Safety and Compliance
The document includes safety warnings regarding laser radiation, specifying compliance with IEC/EN60825-1:2007 standards and FDA performance standards for laser products.

Catalog excerpts

Confocal Microscope

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Confocal Microscope Simple and Robust An essential microscopy laboratory instrument… The C2+ confocal microscope is a basic model within the family of Nikon confocal products. The C2+ is designed as an essential microscopy tool for the laboratory, providing powerful and High-speed acquisition of high-denition images Galvanometer-based high-speed scanning enables confocal imaging of fast, dynamic events in live cells, such as the beating motion of cardiac muscle cells. In traditional confocal systems, fast bi-directional scanning can lead to pixel shifts. However, the C2+ confocal system’s pixel...

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Image Quality High-performance objectives for confocal imaging CFI Apochromat Nikon's unprecedented optics and highly efcient optical design provide the brightest and sharpest images, at the longest working distances. These high-numerical-aperture (NA) objectives provide chromatic aberration correction over a wavelength ranging from ultraviolet to infrared and are ideal for multicolor confocal imaging. In particular, the LWD 40xWI S lens has an extremely wide chromatic aberration correction range from 405 nm to near-IR. The transmission property of these lenses is enhanced through the use of...

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High functionality True Spectral Imaging High-performance imaging software NIS-Elements offers a variety of image processing and analysis functions. It also enables data extraction from acquired images. In addition, NIS-Elements allows for intuitive operation of Nikon microscopes and other third-party peripheral devices, such as EMCCD cameras and lter wheels, to broaden the range of experiments possible. The C2si+ true spectral imaging confocal laser scanning microscope is equipped with two detectors—a standard three-channel uorescence detector and a dedicated spectral detector that allows accurate...

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Unmixing of multiple uorescence In conventional confocal observation, uorescence is visualized as uorescence intensity in a certain wavelength range. The spectral detector allows the conrmation of detailed wavelength characteristics of the uorescence. The C2si+’ spectral detector enables the slight color differences to be conrmed as wavelengths through sensitivity correction. Because wavelength resolution and range are freely selectable, scanning of a uorescence protein with a wide wavelength range from blue to red such as CFP/GFP/YFP/Ds Red is possible at one time. Reference data allows unmixing...

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Laser Units Two types of laser units are available for C2+/C2si+ confocal microscopes. LU-NV Series The compact model consisting of LU-N4, LU-N4S and LU-N3 comes pre-installed with either four (for LU-N4/4S) or three (LU-N3) lasers. The LU-N4/N4S/N3 model achieves maximal light throughput based on its alignment-free, high-efciency laser combiner while maintaining a compact design. The exible model, LU-NV, which also utilizes the high-efciency laser combining system, supports up to eight different lasers and seven output bers, an ideal laser system for multi-modal imaging. The LU-NV laser combiner...

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C2 Plus

C2 Plus

Catalog excerpts

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