ECLIPSE Ti2
24Pages

Inverted Reseaich Microscope ECLIPSE Ti2 Inverted Reseaich Microscope ECLIPSE Ti2

See More Than Be Leading platform for advanced imaging The ECLIPSE Ti2 delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2’s exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2’s unique, intelligent...

Motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal and super-resolution. Manual model with imaging capability for laser applications. Intelligent features provide interactive guidance through imaging workflows and automatically detect microscope status. 3

As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. Development of large-format camera sensors and improvements in the data processing capabilities of PCs have facilitated such research trends. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace. Bright...

Unsurpassed Nikon optics Apodized phase contrast image: BSC-1 cells captured with CFI S Plan Fluor ELWD ADM 40xC objective DIC and epi-fluorescence images: 25mm FOV image of neurons (DAPI, Alexa Fluor 488, Rhodamine-Phalloidin), captured with CFI Plan Apo lambda 60x objective and DS-Qi2 camera Photo courtesy of Josh Rappoport, Nikon Imaging Center, Northwestern Univ.; Sample courtesy of S. Kemal, B. Wang, and R. Vassar, Northwestern Univ. Super-resolution image (DNA PAINT): CV-1 cells expressing α-tubulin (green) and TOMM-20 (magenta) captured with CFI Apo TIRF 100x Oil objective....

Nikon’s high-precision CFI60 infinity optics, designed for use with a variety of sophisticated observation methods, are highly regarded by researchers for their superb optical performance and solid reliability. Apodized phase contrast External phase contrast (Ti2-E) Nikon’s unique apodized phase contrast objectives with selective amplitude filters dramatically increase contrast and reduce halo artifacts to provide detailed highdefinition images. The motorized external phase-contrast system enables users to combine phase contrast with epi-fluorescence imaging without compromising fluorescent...

Even the slightest change in temperature and vibrations in the imaging environment can greatly impact focus stability. The Ti2 eliminates focus drift using both static and dynamic measures to enable faithful visualization of the nanoscopic and microscopic world during long time-lapse experiments. Mechanically redesigned for ultra-high stability (Ti2-E) In order to improve the focusing stability, both Z-drive and PFS autofocusing mechanisms have been completely re-designed. The new Z-focusing mechanism is smaller and positioned adjacent to the nosepiece to minimize vibrations. It remains...

r»ndiensfrQm next button. path. Kah*r«kmnmtk Rtn° Adjustment

It is no longer necessary to memorize complex microscope alignment and operation procedures. The Ti2 integrates data from sensors to guide you through these steps, eliminating user error and enabling researchers to concentrate on their data. Continuous display of microscope status (Ti2-E/A) A collection of built-in sensors detects and relays status information for a variety of components in the microscope. All of the status information is recorded in the metadata when you acquire images with a computer, so you can easily recall acquisition conditions and/or check for configuration errors....

Intuitive operation

The Ti2 has been completely redesigned, from the overall body design to the selection and placement of every button and switch, for the ultimate in user experience. The controls are easy to use even in the dark, where the majority of imaging experiments are conducted. The Ti2 provides an intuitive and effortless user interface so researchers can focus on the data and not on microscope control. Thoughtfully designed layout for microscope control (Ti2-E/A) The placement of all of the buttons and switches are based on the type of illumination they control. Buttons that control diascopic...

Stage up kit Allows expansion of the infinity space for incorporation of additional devices such as a second fluorescence filter turret, barrier filter wheel, back port unit, and LAPP modules. System expandability Epi-fluorescence filter turret Compatible with large FOV. A manual intelligent model and high-speed motorized models with motorized shutter are available. Motorized barrier filter wheel Provides high-speed filter switching (50msec. switch time between adjacent positions) and is compatible with large FOV. It can also be mounted in the infinity space, under the fluorescence filter...

LAPP system Various illumination modules, such as TIRF and photostimulation modules, can be flexibly combined to customize your imaging system. Up to five modules can be mounted simultaneously. Field stop sliders Two different rectangular-shaped apertures and one round aperture model are available. The rectangular models prevent excitation outside of the imaging area and unintentional photobleaching of samples. They can be removed for ultra-wide FOV imaging. Condenser turret Three types are available: manual, intelligent, and a motorized type that automatically switches between seven...

^^Elements A unified acquisition and analysis software platform Multidimensional Imaging Optical Configuration (OC) settings memorize custom observation modes and are combined in the ND Acquisition GUI to easily create experiments combining multi-channe, multi-stage position, z-stacking, and timelapse imaging, as well as image stitching. Other functions such as photostimulation and photobleaching can also be flexibly combined. Nikon's unified software platform, NIS-Elements, provides acquisition control for basic to advanced imaging systems as well as powerful analysis tools and stunning...