Catalog excerpts

N-SIM E - 1

Super Resolution Microscope N-SIM E Super Resolution Microscope

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N-SIM E - 2

A personal super-resolution microscope that provides the same high resolution as the N-SIM Utilizing structured illumination microscopy (SIM) technology, the N-SIM E realizes double the spatial resolution of conventional optical microscopes (to approximately 115 nm). N-SIM E is a streamlined, affordable super-resolution system supporting only essential, commonly used excitation wavelengths and imaging modes, making it an obvious choice for individual labs. Double the resolution of conventional optical microscopes The N-SIM E utilizes Nikon’s innovative new approach to “structured...

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N-SIM E - 3

3-color multi-laser super-resolution capability The compact LU-N3-SIM laser unit dedicated for N-SIM E is installed with the three most commonly used wavelength lasers (488/561/640), enabling super-high resolution imaging in multiple colors. Objectives for super-resolution microscopes The system can be configured with either a 100x oil immersion type, which is suitable for the imaging of fixed samples, or a 60x water immersion type, which is optimal for time-lapse live-cell imaging. The SR (super resolution) objectives have been designed to provide superb optical performance with Nikon’s...

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N-SIM E - 4

Specifications Lateral resolution (FWHM of beads in xy) Axial resolution (FWHM of beads in z) Image acquisition time Compatible objective Up to 1 sec/frame (Slice 3D-SIM) (requires additional 1-2 sec. for calculation) System can be configured with either 100x or 60x CFI SR Apochromat TIRF 100×oil (NA1.49) CFI Apochromat TIRF 100×oil (NA1.49) CFI SR Plan Apochromat IR 60×WI (NA1.27) CFI Plan Apochromat IR 60×WI (NA1.27) ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.) Imaging mode 3D-SIM Reconstruction method: Slice 3D-SIM, Stack 3D-SIM (option) Multi-color imaging NIS-Elements Ar...

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