CASE STUDIES STRUCTURED ILLUMINATION MICROSCOPY (SIM) IMAGING COMPARISON WITH CONFOCAL Tomomi Nemoto, Ph.D. Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Japan Nikon Imaging center in Hokkaido University, Japan Kazuaki Sawada Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Japan sonRyosuke Kawakami, Ph.D. with confocal Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Japan The super-resolution microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along the dendrite has not been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the excitation laser induced by light scattering and optical aberrations. To address this issue and solve these optical problems, we applied a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration to depth of 60um with high spatial resolution is improved in LUCID-treated slices. Furthermore, the structured illumination microscopy shows NIKON CORPORATION a better method thanUnit Healthcare Business confocal microscopy for revealing spine morphologies in single dendrites shown as below. Thus, super-resolution SIM imaging represents a promising high resolution imaging method for neuroscience. Reference European Journal of Neuroscience, Vol. 47, pp. 1033–1042, 2018 Ryosuke Kawakami, Ph.D.*2 , Tomomi Nemoto, Ph.D.*1,*3 Microscopy Structured Illumination ular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Japan for Pathogenesis, Graduate School of Medicine, Ehime University, Japan er in Hokkaido University, Japan n microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along t been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the uced by light scattering and optical aberrations. To address this issue and solve these optical ed a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration to depth of patial resolution is improved in LUCID-treated slices. Furthermore, the structured illumination a better method than confocal microscopy for revealing spine morphologies in single dendrites Thus, super-resolution SIM imaging represents a promising high resolution imaging method for Confocal microscopy Confocal Microscop

Structured Illumination Microscopy (SIM) Imaging Comparison With Confocal Dr. Jun Kawamoto Assistant Professor of Institute for Chemical Research, Kyoto University The SIM method is an accepted technology in the field of bioscience imaging. We think that it has good point to capture the biological sample image as below. 1) N-SIM is suitable for observing the distribution of bacterial membrane phospholipids and cell division proteins. 2) N-SIM image clearly show the fine structure of a cell division protein which is usually observed as blurred structure in confocal microscopy image. 3) N-SIM can...

Structured Illumination Microscopy (SIM) Imaging Comparison With Confocal Sample: Human epidermal keratinocytes Department of Morphological Biology, Fukuoka Dental College Laboratory URL: http://www.fdcnet.ac.jp/col/info/teacher/ kouza/kouzou.html The SIM method is an accepted technology in the field of bioscience imaging. We think that it has good point to capture the biological sample image as below. 1) N-SIM is suitable for observing the distribution change of proteins constituting the cytoskeleton. 2) N-SIM image clearly show the structure of cell margin and fine cytoskeleton which is usually...

Structured Illumination Microscopy (SIM) Imaging Comparison With Confocal Dr. Yoshihiro Inoue Tsuyoshi Shoda and Yoshihiro Inoue, Department of Insect Biomedical Research, Kyoto Institute of Technology Laboratory URL: https://www.kit.ac.jp/en/research/ department-of-insect-biomedical-research/ The SIM method is an accepted technology in the field of bioscience imaging. We think that it has good point to capture the biological sample image as below. Structured Illumination Microscopy 1) N-SIM allows us to observe intracellular fine structures such as centrioles which are less than 1 um in length...