Group: Nikon
Catalog excerpts
Super Resolution Microscopes
Open the catalog to page 1_N-SIM S_ Structured illumination microscopy 15 fps image acquisition Lateral resolution of -115 nm Axial resolution of ~269 nm
Open the catalog to page 2The N-SIM S Super Resolution Microscope utilizes a unique high-speed structured illumination system to achieve acquisition speeds of up to 15 fps*, enabling fast biological processes to be captured at twice the spatial resolution of conventional light microscopes (~115nm** in XY). The N-STORM Super Resolution Microscope achieves a 10-fold improvement in resolution compared to conventional light microscopes (~20 nm in XY), enabling observation at the true molecular level. The N-SIM S and N-STORM can be easily combined within the same imaging system for greater flexibility in nanoscale...
Open the catalog to page 3Lamellipodia of NG108 cell labeled with Alexa Fluor® 488 for actin (green) and TRITC-Phalloidin for microtubules (red). Photo courtesy of: Drs. Shizuha Ishiyama and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) LLC-PK1 cell labeled with DAPI for nucleus (blue), Alexa Fluor® 488 for microtubules (green) and TRITC-Phalloidin for actin (red). Photo courtesy of: Dr. Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) See life in super resolution
Open the catalog to page 4Synaptonemal complexes of C. elegans pachytene germ cells labeled with anti-SYP-1 antibodies. Photo courtesy of: Tyler Machovina and Dr. Judith Yanowitz, Magee-Womens Research Institute. Malaria parasite surface (MTIP) labeled with Alexa Fluor ® 488 (green), Erythrocyte membrane (Band 3) labeled with Alexa Fluor ® 568 (red), DNA labeled with DAPI (blue) Scientific Reports DOI:10.1038/s41598-018-22026-0 Photo courtesy of: Drs. Masayuki Morita, Eizo Takashima, Tadahiro Iimura, Takafumi Tsuboi, Proteo-Science Center, Ehime University The N-SIM S combines innovative structured illumination...
Open the catalog to page 5Endosomes of COS7 cell labeled with YFP. Rapid movement of endosomes is captured at high resolution. Image acquisition speed: 6 fps Imaging mode: 3D-SIM Image courtesy of: Yasushi Okada, M.D., Ph.D., Department of Physics, Graduate School of Science, The University of Tokyo Scan the QR code to view a video illustrating super-resolution and widefield images. Capture rapid changes in live cells High-speed super-resolution imaging at 15 fps Nikon’s new high-speed structured illumination system utilizes a novel pattern modulation technology to generate fast and precise switching of illumination...
Open the catalog to page 6Growth cone of NG108 cell labeled with GFP-Lifeact for F-actin. Formation of actin mesh is captured at high-speed. Image acquisition speed: 10 fps Imaging mode: TIRF-SIM Image courtesy of: Drs. Minami Tanaka and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) Scan the QR code to view a video illustrating super-resolution and widefield images. Histone H2B-GFP expressing in a HeLa cell. Visualization of fine movements of chromatin domains in different locations. Image acquisition speed: 3.9 fps Imaging mode: 3D-SIM Image courtesy of: Yuko Sato, Ph.D....
Open the catalog to page 7Two-color TIRF-SIM imaging of growth cone of NG108 cell labeled with Alexa Fluor ® 488 for F-actin (green) and Alexa Fluor ® 555 for microtubules (orange) Reconstructed image size: 2048 x 2048 pixels (66 μm x 66 μm with a 100X objective) Sample courtesy of: Drs. Shizuha Ishiyama and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) Easily switch between imaging modes for optimal results Automatic switching between illumination modes Newly-developed, high-speed structured illumination technology not only enables fast acquisition rates but also automatic...
Open the catalog to page 8Simultaneous two-channel imaging Simultaneous two-color imaging is possible by utilizing an optional Two Camera Imaging Adaptor* and two sCMOS cameras. * Andor Technology Ltd. Growth cone of NG108 cell expressing GFP-LifeAct (F-actin, green) and mCherry-tubulin (microtubules, red) Photo courtesy of: Dr. Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) 2D-SIM mode/TIRF-SIM mode This mode captures super-resolution 2D images at high speed with incredible contrast. The TIRF-SIM mode enables Total Internal Reflection Fluorescence observation at double the...
Open the catalog to page 9“N-SIM provides the resolution necessary to identify and evaluate the structural organization of the nuclear lamina*1, *2. Its ease of use and stable performance has made N-SIM an integral research tool in my laboratory.” *1 ol Biol Cell. 2015 Nov 5; 26(22):4075-86. M *2 Nature . 2017 Mar 9; 543(7644):261-264. Dr. Robert D. Goldman Ellison Foundation Senior Scholar, Stephen Walter Ranson Professor, Chair, Dept. of Cell & Mol. Biol., Feinberg School of Medicine, Northwestern University Lamin B1 (red) and Lamin C (green) form separate but interacting meshworks within the lamina of the...
Open the catalog to page 10Ti2-E with double layer configuration with Perfect Focus Unit Motorized HG fiber illuminator Intensilight TI2-LA-HTIRF H-TIRF module with TI2-LA-FL Epi-Fl module TI2-LA-NS2 N-STORM module 2 with TI2-LA-FL Epi-Fl module** TI2-FT N-SIM motorizedfilter turret LU-NV series laser unit ORCA-Flash4.0 sCMOS camera (Hamamatsu Photonics K.K.) * Required when used with confocal system * Required when configured with N-STORM *** Supplied with microscope main body N-SIM S Specifications Lateral resolution (FWHM of beads in xy) *1 These values are measured using 100 nm diameter beads excited by a 488 nm...
Open the catalog to page 11a: Hippocampal neurons and glia in culture b: Growth cone of a neuron in culture Experience the nanoscale universe
Open the catalog to page 12c: Glia in a neuronal culture d: COS cells 3D-STORM imaging of actin labeled with Alexa Fluor ® 647 Phalloidin using depth-code pseudo color. Image “a” shows four types of actin organization, from bottom left to top right: the cell body of a neuron, a glial cell with stress fibers, a neuronal dendrite with spines, and an axon. Photos courtesy of: Dr. Christophe Leterrier, NeuroCyto team, NICN CNRS-AMU UMR7259, Marseille, France STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution image by combining precise localization information for individual fluorophores...
Open the catalog to page 13All Nikon Instruments catalogs and technical brochures
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HCA
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DS-Fi3
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ShuttlePix
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A1 MP+ / A1R MP
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A1 HD25 / A1R HD25
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TI-E
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ECLIPSE TS2
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ECLIPSE TS2R
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N-SIME
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N-SIM N-STORM
24 Pages
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ECLIPSE E100 LED
8 Pages
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Bio Station IMQ
8 Pages
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ECLIPSE Ti2
24 Pages
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A1 MP+ / A1R MP+
20 Pages
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DIGITAL SIGHT SERIES
16 Pages
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Eclipse LV100N-POL Ci-POL
8 Pages
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ShuttlePix Brochure
5 Pages
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NeoScope Brochure
16 Pages
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Eclipse FN1
12 Pages
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Eclipse Ti
32 Pages
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C2 Plus
16 Pages
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A1+ / A1R+
13 Pages
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Biological Microscopes
24 Pages
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Stereomicroscopes
32 Pages
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SMZ18
16 Pages
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N-SIM E
4 Pages
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N-STORM 4.1
13 Pages
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A1_MP
11 Pages
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AZ_C1
2 Pages
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MiBrochure
2 Pages
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A1+_A1R+_2CE-SBTH-9
13 Pages
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C2+_2CE-SCHH-5
16 Pages
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High Content Microscopy
2 Pages
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NIS-Elements
8 Pages
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Digital Sight Series
9 Pages
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ShuttlePix P-400R
5 Pages
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AZ100M
8 Pages
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AZ100
4 Pages
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Eclipse E200POL
3 Pages
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Eclipse LV100ND POL/DS
2 Pages
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Eclipse E100 LED
4 Pages
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Eclipse Ci-E/ Ci-L
5 Pages
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Eclipse Ni
15 Pages
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Eclipse E200 POL
3 Pages
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Biological Microscopes
12 Pages
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ECLIPSE LV100N POL 50i/ POL
4 Pages
Archived catalogs
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Digital Sight Series Brochure
16 Pages
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Eclipse E100-LED
8 Pages
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A1 MP+ / A1R MP+
11 Pages